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MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis

This study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with sero...

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Autores principales: Ayo, Christiane Maria, Camargo, Ana Vitória da Silveira, Frederico, Fábio Batista, Siqueira, Rubens Camargo, Previato, Mariana, Murata, Fernando Henrique Antunes, Silveira-Carvalho, Aparecida Perpétuo, Barbosa, Amanda Pires, Brandão de Mattos, Cinara de Cássia, de Mattos, Luiz Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4682939/
https://www.ncbi.nlm.nih.gov/pubmed/26672749
http://dx.doi.org/10.1371/journal.pone.0144534
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author Ayo, Christiane Maria
Camargo, Ana Vitória da Silveira
Frederico, Fábio Batista
Siqueira, Rubens Camargo
Previato, Mariana
Murata, Fernando Henrique Antunes
Silveira-Carvalho, Aparecida Perpétuo
Barbosa, Amanda Pires
Brandão de Mattos, Cinara de Cássia
de Mattos, Luiz Carlos
author_facet Ayo, Christiane Maria
Camargo, Ana Vitória da Silveira
Frederico, Fábio Batista
Siqueira, Rubens Camargo
Previato, Mariana
Murata, Fernando Henrique Antunes
Silveira-Carvalho, Aparecida Perpétuo
Barbosa, Amanda Pires
Brandão de Mattos, Cinara de Cássia
de Mattos, Luiz Carlos
author_sort Ayo, Christiane Maria
collection PubMed
description This study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with serological diagnosis of toxoplasmosis. Participants were classified into two distinct groups after conducting fundoscopic exams according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis. The group of patients with scars/lesions was further subdivided into two groups according to the type of the ocular manifestation observed: primary (n = 120) or recurrent (n = 28). Genotyping of the MICA and HLA alleles was performed by the polymerase chain reaction-sequence specific oligonucleotide technique (PCR-SSO; One Lambda®) and the MICA-129 polymorphism (rs1051792) was identified by nested polymerase chain reaction (PCR-RFLP). Significant associations involving MICA polymorphisms were not found. Although the MICA*002~HLA-B*35 haplotype was associated with increased risk of developing ocular toxoplasmosis (P-value = 0.04; OR = 2.20; 95% CI = 1.05–4.60), and the MICA*008~HLA-C*07 haplotype was associated with protection against the development of manifestations of ocular toxoplasmosis (P-value = 0.009; OR: 0.44; 95% CI: 0.22–0.76), these associations were not statistically significant after adjusting for multiple comparisons. MICA polymorphisms do not appear to influence the development of ocular lesions in patients diagnosed with toxoplasmosis in this study population.
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spelling pubmed-46829392015-12-31 MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis Ayo, Christiane Maria Camargo, Ana Vitória da Silveira Frederico, Fábio Batista Siqueira, Rubens Camargo Previato, Mariana Murata, Fernando Henrique Antunes Silveira-Carvalho, Aparecida Perpétuo Barbosa, Amanda Pires Brandão de Mattos, Cinara de Cássia de Mattos, Luiz Carlos PLoS One Research Article This study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with serological diagnosis of toxoplasmosis. Participants were classified into two distinct groups after conducting fundoscopic exams according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis. The group of patients with scars/lesions was further subdivided into two groups according to the type of the ocular manifestation observed: primary (n = 120) or recurrent (n = 28). Genotyping of the MICA and HLA alleles was performed by the polymerase chain reaction-sequence specific oligonucleotide technique (PCR-SSO; One Lambda®) and the MICA-129 polymorphism (rs1051792) was identified by nested polymerase chain reaction (PCR-RFLP). Significant associations involving MICA polymorphisms were not found. Although the MICA*002~HLA-B*35 haplotype was associated with increased risk of developing ocular toxoplasmosis (P-value = 0.04; OR = 2.20; 95% CI = 1.05–4.60), and the MICA*008~HLA-C*07 haplotype was associated with protection against the development of manifestations of ocular toxoplasmosis (P-value = 0.009; OR: 0.44; 95% CI: 0.22–0.76), these associations were not statistically significant after adjusting for multiple comparisons. MICA polymorphisms do not appear to influence the development of ocular lesions in patients diagnosed with toxoplasmosis in this study population. Public Library of Science 2015-12-16 /pmc/articles/PMC4682939/ /pubmed/26672749 http://dx.doi.org/10.1371/journal.pone.0144534 Text en © 2015 Ayo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ayo, Christiane Maria
Camargo, Ana Vitória da Silveira
Frederico, Fábio Batista
Siqueira, Rubens Camargo
Previato, Mariana
Murata, Fernando Henrique Antunes
Silveira-Carvalho, Aparecida Perpétuo
Barbosa, Amanda Pires
Brandão de Mattos, Cinara de Cássia
de Mattos, Luiz Carlos
MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis
title MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis
title_full MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis
title_fullStr MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis
title_full_unstemmed MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis
title_short MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis
title_sort mhc class i chain-related gene a polymorphisms and linkage disequilibrium with hla-b and hla-c alleles in ocular toxoplasmosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4682939/
https://www.ncbi.nlm.nih.gov/pubmed/26672749
http://dx.doi.org/10.1371/journal.pone.0144534
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