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Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana
To elucidate the molecular mechanisms of stomatal opening and closure, we performed a genetic screen using infrared thermography to isolate stomatal aperture mutants. We identified a mutant designated low temperature with open-stomata 1 (lost1), which exhibited reduced leaf temperature, wider stomat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Japan
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683165/ https://www.ncbi.nlm.nih.gov/pubmed/24840863 http://dx.doi.org/10.1007/s10265-014-0636-0 |
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author | Tomiyama, Masakazu Inoue, Shin-ichiro Tsuzuki, Tomo Soda, Midori Morimoto, Sayuri Okigaki, Yukiko Ohishi, Takaya Mochizuki, Nobuyoshi Takahashi, Koji Kinoshita, Toshinori |
author_facet | Tomiyama, Masakazu Inoue, Shin-ichiro Tsuzuki, Tomo Soda, Midori Morimoto, Sayuri Okigaki, Yukiko Ohishi, Takaya Mochizuki, Nobuyoshi Takahashi, Koji Kinoshita, Toshinori |
author_sort | Tomiyama, Masakazu |
collection | PubMed |
description | To elucidate the molecular mechanisms of stomatal opening and closure, we performed a genetic screen using infrared thermography to isolate stomatal aperture mutants. We identified a mutant designated low temperature with open-stomata 1 (lost1), which exhibited reduced leaf temperature, wider stomatal aperture, and a pale green phenotype. Map-based analysis of the LOST1 locus revealed that the lost1 mutant resulted from a missense mutation in the Mg-chelatase I subunit 1 (CHLI1) gene, which encodes a subunit of the Mg-chelatase complex involved in chlorophyll synthesis. Transformation of the wild-type CHLI1 gene into lost1 complemented all lost1 phenotypes. Stomata in lost1 exhibited a partial ABA-insensitive phenotype similar to that of rtl1, a Mg-chelatase H subunit missense mutant. The Mg-protoporphyrin IX methyltransferase (CHLM) gene encodes a subsequent enzyme in the chlorophyll synthesis pathway. We examined stomatal movement in a CHLM knockdown mutant, chlm, and found that it also exhibited an ABA-insensitive phenotype. However, lost1 and chlm seedlings all showed normal expression of ABA-induced genes, such as RAB18 and RD29B, in response to ABA. These results suggest that the chlorophyll synthesis enzymes, Mg-chelatase complex and CHLM, specifically affect ABA signaling in the control of stomatal aperture and have no effect on ABA-induced gene expression. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10265-014-0636-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4683165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-46831652015-12-23 Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana Tomiyama, Masakazu Inoue, Shin-ichiro Tsuzuki, Tomo Soda, Midori Morimoto, Sayuri Okigaki, Yukiko Ohishi, Takaya Mochizuki, Nobuyoshi Takahashi, Koji Kinoshita, Toshinori J Plant Res Regular Paper To elucidate the molecular mechanisms of stomatal opening and closure, we performed a genetic screen using infrared thermography to isolate stomatal aperture mutants. We identified a mutant designated low temperature with open-stomata 1 (lost1), which exhibited reduced leaf temperature, wider stomatal aperture, and a pale green phenotype. Map-based analysis of the LOST1 locus revealed that the lost1 mutant resulted from a missense mutation in the Mg-chelatase I subunit 1 (CHLI1) gene, which encodes a subunit of the Mg-chelatase complex involved in chlorophyll synthesis. Transformation of the wild-type CHLI1 gene into lost1 complemented all lost1 phenotypes. Stomata in lost1 exhibited a partial ABA-insensitive phenotype similar to that of rtl1, a Mg-chelatase H subunit missense mutant. The Mg-protoporphyrin IX methyltransferase (CHLM) gene encodes a subsequent enzyme in the chlorophyll synthesis pathway. We examined stomatal movement in a CHLM knockdown mutant, chlm, and found that it also exhibited an ABA-insensitive phenotype. However, lost1 and chlm seedlings all showed normal expression of ABA-induced genes, such as RAB18 and RD29B, in response to ABA. These results suggest that the chlorophyll synthesis enzymes, Mg-chelatase complex and CHLM, specifically affect ABA signaling in the control of stomatal aperture and have no effect on ABA-induced gene expression. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10265-014-0636-0) contains supplementary material, which is available to authorized users. Springer Japan 2014-05-20 2014 /pmc/articles/PMC4683165/ /pubmed/24840863 http://dx.doi.org/10.1007/s10265-014-0636-0 Text en © The Botanical Society of Japan and Springer Japan 2014 |
spellingShingle | Regular Paper Tomiyama, Masakazu Inoue, Shin-ichiro Tsuzuki, Tomo Soda, Midori Morimoto, Sayuri Okigaki, Yukiko Ohishi, Takaya Mochizuki, Nobuyoshi Takahashi, Koji Kinoshita, Toshinori Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana |
title | Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana |
title_full | Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana |
title_fullStr | Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana |
title_full_unstemmed | Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana |
title_short | Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana |
title_sort | mg-chelatase i subunit 1 and mg-protoporphyrin ix methyltransferase affect the stomatal aperture in arabidopsis thaliana |
topic | Regular Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683165/ https://www.ncbi.nlm.nih.gov/pubmed/24840863 http://dx.doi.org/10.1007/s10265-014-0636-0 |
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