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Markerless Escherichia coli rrn Deletion Strains for Genetic Determination of Ribosomal Binding Sites

Single-copy rrn strains facilitate genetic ribosomal studies in Escherichia coli. Consecutive markerless deletion of rrn operons resulted in slower growth upon inactivation of the fourth copy, which was reversed by supplying transfer RNA genes encoded in rrn operons in trans. Removal of the sixth, p...

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Detalles Bibliográficos
Autores principales:	Quan, Selwyn, Skovgaard, Ole, McLaughlin, Robert E., Buurman, Ed T., Squires, Catherine L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Genetics Society of America 2015
Materias:	Investigations
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683628/ https://www.ncbi.nlm.nih.gov/pubmed/26438293 http://dx.doi.org/10.1534/g3.115.022301

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