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Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms

Drosophila willistoni is a geographically widespread Neotropical species. The genome of strain Gd-H4-1 from Guadeloupe Island (Caribbean) was sequenced in 2007 as part of the 12 Drosophila Genomes Project. The assembled scaffolds were joined based on conserved linkage and assigned to polytene chromo...

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Autores principales: Garcia, Carolina, Delprat, Alejandra, Ruiz, Alfredo, Valente, Vera L. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683629/
https://www.ncbi.nlm.nih.gov/pubmed/26438290
http://dx.doi.org/10.1534/g3.115.021311
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author Garcia, Carolina
Delprat, Alejandra
Ruiz, Alfredo
Valente, Vera L. S.
author_facet Garcia, Carolina
Delprat, Alejandra
Ruiz, Alfredo
Valente, Vera L. S.
author_sort Garcia, Carolina
collection PubMed
description Drosophila willistoni is a geographically widespread Neotropical species. The genome of strain Gd-H4-1 from Guadeloupe Island (Caribbean) was sequenced in 2007 as part of the 12 Drosophila Genomes Project. The assembled scaffolds were joined based on conserved linkage and assigned to polytene chromosomes based on a handful of genetic and physical markers. This paucity of markers was particularly striking in the metacentric chromosome II, comprised two similarly sized arms, IIL and IIR, traditionally considered homologous to Muller elements C and B, respectively. In this paper we present the cytological mapping of 22 new gene markers to increase the number of markers mapped by in situ hybridization and to test the assignment of scaffolds to the polytene chromosome II arms. For this purpose, we generated, by polymerase chain reaction amplification, one or two gene probes from each scaffold assigned to the chromosome II arms and mapped these probes to the Gd-H4-1 strain’s polytene chromosomes by nonfluorescent in situ hybridization. Our findings show that chromosome arms IIL and IIR correspond to Muller elements B and C, respectively, directly contrasting the current homology assignments in D. willistoni and constituting a major reassignment of the scaffolds to chromosome II arms.
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spelling pubmed-46836292015-12-18 Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms Garcia, Carolina Delprat, Alejandra Ruiz, Alfredo Valente, Vera L. S. G3 (Bethesda) Investigations Drosophila willistoni is a geographically widespread Neotropical species. The genome of strain Gd-H4-1 from Guadeloupe Island (Caribbean) was sequenced in 2007 as part of the 12 Drosophila Genomes Project. The assembled scaffolds were joined based on conserved linkage and assigned to polytene chromosomes based on a handful of genetic and physical markers. This paucity of markers was particularly striking in the metacentric chromosome II, comprised two similarly sized arms, IIL and IIR, traditionally considered homologous to Muller elements C and B, respectively. In this paper we present the cytological mapping of 22 new gene markers to increase the number of markers mapped by in situ hybridization and to test the assignment of scaffolds to the polytene chromosome II arms. For this purpose, we generated, by polymerase chain reaction amplification, one or two gene probes from each scaffold assigned to the chromosome II arms and mapped these probes to the Gd-H4-1 strain’s polytene chromosomes by nonfluorescent in situ hybridization. Our findings show that chromosome arms IIL and IIR correspond to Muller elements B and C, respectively, directly contrasting the current homology assignments in D. willistoni and constituting a major reassignment of the scaffolds to chromosome II arms. Genetics Society of America 2015-10-04 /pmc/articles/PMC4683629/ /pubmed/26438290 http://dx.doi.org/10.1534/g3.115.021311 Text en Copyright © 2015 Garcia et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Garcia, Carolina
Delprat, Alejandra
Ruiz, Alfredo
Valente, Vera L. S.
Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms
title Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms
title_full Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms
title_fullStr Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms
title_full_unstemmed Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms
title_short Reassignment of Drosophila willistoni Genome Scaffolds to Chromosome II Arms
title_sort reassignment of drosophila willistoni genome scaffolds to chromosome ii arms
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4683629/
https://www.ncbi.nlm.nih.gov/pubmed/26438290
http://dx.doi.org/10.1534/g3.115.021311
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