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Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates

Bacterial genomes accommodate a variety of mobile genetic elements, including bacteriophage-related clusters that encode phage tail-like protein complexes playing a role in interactions with eukaryotic or prokaryotic cells. Such tailocins are unable to replicate inside target cells due to the lack o...

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Autores principales: Ghequire, Maarten G.K., Dillen, Yörg, Lambrichts, Ivo, Proost, Paul, Wattiez, Ruddy, De Mot, René
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684702/
https://www.ncbi.nlm.nih.gov/pubmed/26412856
http://dx.doi.org/10.1093/gbe/evv184
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author Ghequire, Maarten G.K.
Dillen, Yörg
Lambrichts, Ivo
Proost, Paul
Wattiez, Ruddy
De Mot, René
author_facet Ghequire, Maarten G.K.
Dillen, Yörg
Lambrichts, Ivo
Proost, Paul
Wattiez, Ruddy
De Mot, René
author_sort Ghequire, Maarten G.K.
collection PubMed
description Bacterial genomes accommodate a variety of mobile genetic elements, including bacteriophage-related clusters that encode phage tail-like protein complexes playing a role in interactions with eukaryotic or prokaryotic cells. Such tailocins are unable to replicate inside target cells due to the lack of a phage head with associated DNA. A subset of tailocins mediate antagonistic activities with bacteriocin-like specificity. Functional characterization of bactericidal tailocins of two Pseudomonas putida rhizosphere isolates revealed not only extensive similarity with the tail assembly module of the Pseudomonas aeruginosa R-type pyocins but also differences in genomic integration site, regulatory genes, and lytic release modules. Conversely, these three features are quite similar between strains of the P. putida and Pseudomonas fluorescens clades, although phylogenetic analysis of tail genes suggests them to have evolved separately. Unlike P. aeruginosa R pyocin elements, the tailocin gene clusters of other pseudomonads frequently carry cargo genes, including bacteriocins. Compared with P. aeruginosa, the tailocin tail fiber sequences that act as specificity determinants have diverged much more extensively among the other pseudomonad species, mostly isolates from soil and plant environments. Activity of the P. putida antibacterial particles requires a functional lipopolysaccharide layer on target cells, but contrary to R pyocins from P. aeruginosa, strain susceptibilities surpass species boundaries.
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spelling pubmed-46847022015-12-22 Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates Ghequire, Maarten G.K. Dillen, Yörg Lambrichts, Ivo Proost, Paul Wattiez, Ruddy De Mot, René Genome Biol Evol Research Articles Bacterial genomes accommodate a variety of mobile genetic elements, including bacteriophage-related clusters that encode phage tail-like protein complexes playing a role in interactions with eukaryotic or prokaryotic cells. Such tailocins are unable to replicate inside target cells due to the lack of a phage head with associated DNA. A subset of tailocins mediate antagonistic activities with bacteriocin-like specificity. Functional characterization of bactericidal tailocins of two Pseudomonas putida rhizosphere isolates revealed not only extensive similarity with the tail assembly module of the Pseudomonas aeruginosa R-type pyocins but also differences in genomic integration site, regulatory genes, and lytic release modules. Conversely, these three features are quite similar between strains of the P. putida and Pseudomonas fluorescens clades, although phylogenetic analysis of tail genes suggests them to have evolved separately. Unlike P. aeruginosa R pyocin elements, the tailocin gene clusters of other pseudomonads frequently carry cargo genes, including bacteriocins. Compared with P. aeruginosa, the tailocin tail fiber sequences that act as specificity determinants have diverged much more extensively among the other pseudomonad species, mostly isolates from soil and plant environments. Activity of the P. putida antibacterial particles requires a functional lipopolysaccharide layer on target cells, but contrary to R pyocins from P. aeruginosa, strain susceptibilities surpass species boundaries. Oxford University Press 2015-09-26 /pmc/articles/PMC4684702/ /pubmed/26412856 http://dx.doi.org/10.1093/gbe/evv184 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Articles
Ghequire, Maarten G.K.
Dillen, Yörg
Lambrichts, Ivo
Proost, Paul
Wattiez, Ruddy
De Mot, René
Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates
title Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates
title_full Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates
title_fullStr Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates
title_full_unstemmed Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates
title_short Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates
title_sort different ancestries of r tailocins in rhizospheric pseudomonas isolates
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684702/
https://www.ncbi.nlm.nih.gov/pubmed/26412856
http://dx.doi.org/10.1093/gbe/evv184
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