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DNA damage and cellular abnormalities in tuberculosis, lung cancer and chronic obstructive pulmonary disease

BACKGROUND: Tuberculosis (TB), Lung Cancer (LC) and Chronic Obstructive Pulmonary Diseases (COPD) affect millions of individuals worldwide. Monitoring of DNA damage in pathological situations has been investigated because it can add a new dimension to clinical expression and may represent a potentia...

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Detalles Bibliográficos
Autores principales: da Silva, Andréa Lúcia Gonçalves, Bresciani, Maribel Josimara, Karnopp, Thaís Evelyn, Weber, Augusto Ferreira, Ellwanger, Joel Henrique, Henriques, João Antonio Pêgas, Valim, Andréia Rosane de Moura, Possuelo, Lia Gonçalves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4684909/
https://www.ncbi.nlm.nih.gov/pubmed/26688728
http://dx.doi.org/10.1186/s40248-015-0034-z
Descripción
Sumario:BACKGROUND: Tuberculosis (TB), Lung Cancer (LC) and Chronic Obstructive Pulmonary Diseases (COPD) affect millions of individuals worldwide. Monitoring of DNA damage in pathological situations has been investigated because it can add a new dimension to clinical expression and may represent a potential target for therapeutic intervention. The aim of this study was to evaluate DNA damage and the frequency of cellular abnormalities in TB, LC and COPD patients by comparing them to healthy subjects. METHODS: The detection of DNA damage by a buccal micronucleus cytome assay was investigated in patients with COPD (n = 28), LC (n = 18) and TB (n = 22) and compared to control individuals (n = 17). RESULTS: The COPD group had a higher frequency of apoptotic cells compared to TB and LC group. The TB group showed a higher frequency of DNA damage, defect in cytokinesis, apoptotic and necrotic cells. Patients with LC had low frequency of chromosomal aberrations than TB and COPD patients. CONCLUSION: COPD patients showed cellular abnormalities that corresponded to cell death by apoptosis and necrosis, while patients with TB presented defects in cytokinesis and dysfunctions in DNA repair that resulted in the formation of micronucleus (MN) besides apoptotic and necrotic cells. Patients with COPD, TB and LC had a low frequency of permanent DNA damage.