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Impact of a confirmatory RhD test on the correct serologic typing of blood donors
BACKGROUND: The RHD gene is highly polymorphic, which results in a large number of RhD variant phenotypes. Discrepancies in RhD typing are still a problem in blood banks and increase the risk of alloimmunization. In this study, the RhD typing strategy at a blood bank in Brazil was evaluated. METHODS...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Hematologia e Hemoterapia
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685092/ https://www.ncbi.nlm.nih.gov/pubmed/26408363 http://dx.doi.org/10.1016/j.bjhh.2015.06.001 |
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author | Schmidt, Luciana Cayres Castilho, Lilian Vieira, Otavio Vinicius Neves Sippert, Emília Gaspardi, Ane Caroline Martins, Marina Lobato da Silva Malta, Maria Clara Fernandes |
author_facet | Schmidt, Luciana Cayres Castilho, Lilian Vieira, Otavio Vinicius Neves Sippert, Emília Gaspardi, Ane Caroline Martins, Marina Lobato da Silva Malta, Maria Clara Fernandes |
author_sort | Schmidt, Luciana Cayres |
collection | PubMed |
description | BACKGROUND: The RHD gene is highly polymorphic, which results in a large number of RhD variant phenotypes. Discrepancies in RhD typing are still a problem in blood banks and increase the risk of alloimmunization. In this study, the RhD typing strategy at a blood bank in Brazil was evaluated. METHODS: One-hundred and fifty-two samples typed as RhD negative and C or E positive by routine tests (automated system and indirect antiglobulin test using the tube technique) were reevaluated for RhD status by three methods. The method with the best performance was implemented and evaluated for a period of one year (n = 4897 samples). Samples that were D positive exclusively in the confirmatory test were submitted to molecular analysis. RESULTS: The gel test for indirect antiglobulin testing with anti-D immunoglobulin G (clone ESD1) presented the best results. Seventy samples (1.43%) previously typed as RhD negative showed reactivity in the gel test for indirect antiglobulin testing and were reclassified as D positive. D variants that may cause alloimmunization, such as weak D type 2 and partial D(VI), were detected. CONCLUSION: The confirmatory RhD test using the gel test for indirect antiglobulin testing represents a breakthrough in transfusion safety in this blood center. Our results emphasize the importance of assessing the blood group typing strategy in blood banks. |
format | Online Article Text |
id | pubmed-4685092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Hematologia e Hemoterapia |
record_format | MEDLINE/PubMed |
spelling | pubmed-46850922016-01-15 Impact of a confirmatory RhD test on the correct serologic typing of blood donors Schmidt, Luciana Cayres Castilho, Lilian Vieira, Otavio Vinicius Neves Sippert, Emília Gaspardi, Ane Caroline Martins, Marina Lobato da Silva Malta, Maria Clara Fernandes Rev Bras Hematol Hemoter Original Article BACKGROUND: The RHD gene is highly polymorphic, which results in a large number of RhD variant phenotypes. Discrepancies in RhD typing are still a problem in blood banks and increase the risk of alloimmunization. In this study, the RhD typing strategy at a blood bank in Brazil was evaluated. METHODS: One-hundred and fifty-two samples typed as RhD negative and C or E positive by routine tests (automated system and indirect antiglobulin test using the tube technique) were reevaluated for RhD status by three methods. The method with the best performance was implemented and evaluated for a period of one year (n = 4897 samples). Samples that were D positive exclusively in the confirmatory test were submitted to molecular analysis. RESULTS: The gel test for indirect antiglobulin testing with anti-D immunoglobulin G (clone ESD1) presented the best results. Seventy samples (1.43%) previously typed as RhD negative showed reactivity in the gel test for indirect antiglobulin testing and were reclassified as D positive. D variants that may cause alloimmunization, such as weak D type 2 and partial D(VI), were detected. CONCLUSION: The confirmatory RhD test using the gel test for indirect antiglobulin testing represents a breakthrough in transfusion safety in this blood center. Our results emphasize the importance of assessing the blood group typing strategy in blood banks. Sociedade Brasileira de Hematologia e Hemoterapia 2015 2015-07-09 /pmc/articles/PMC4685092/ /pubmed/26408363 http://dx.doi.org/10.1016/j.bjhh.2015.06.001 Text en © 2015 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. All rights reserved. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Schmidt, Luciana Cayres Castilho, Lilian Vieira, Otavio Vinicius Neves Sippert, Emília Gaspardi, Ane Caroline Martins, Marina Lobato da Silva Malta, Maria Clara Fernandes Impact of a confirmatory RhD test on the correct serologic typing of blood donors |
title | Impact of a confirmatory RhD test on the correct serologic typing of blood donors |
title_full | Impact of a confirmatory RhD test on the correct serologic typing of blood donors |
title_fullStr | Impact of a confirmatory RhD test on the correct serologic typing of blood donors |
title_full_unstemmed | Impact of a confirmatory RhD test on the correct serologic typing of blood donors |
title_short | Impact of a confirmatory RhD test on the correct serologic typing of blood donors |
title_sort | impact of a confirmatory rhd test on the correct serologic typing of blood donors |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685092/ https://www.ncbi.nlm.nih.gov/pubmed/26408363 http://dx.doi.org/10.1016/j.bjhh.2015.06.001 |
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