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Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin
The present study aimed at biochemical and molecular characterization of Escherichia coli strains isolated from horticultural products and irrigation water of Cotonou. The samples were collected from 12 market gardeners of 4 different sites. Rapid' E. coli medium was used for identification of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685095/ https://www.ncbi.nlm.nih.gov/pubmed/26770972 http://dx.doi.org/10.1155/2015/286473 |
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author | Moussé, Wassiyath Sina, Haziz Baba-Moussa, Farid Noumavo, Pacôme A. Agbodjato, Nadège A. Adjanohoun, Adolphe Baba-Moussa, Lamine |
author_facet | Moussé, Wassiyath Sina, Haziz Baba-Moussa, Farid Noumavo, Pacôme A. Agbodjato, Nadège A. Adjanohoun, Adolphe Baba-Moussa, Lamine |
author_sort | Moussé, Wassiyath |
collection | PubMed |
description | The present study aimed at biochemical and molecular characterization of Escherichia coli strains isolated from horticultural products and irrigation water of Cotonou. The samples were collected from 12 market gardeners of 4 different sites. Rapid' E. coli medium was used for identification of E. coli strains and the antimicrobial susceptibility was performed by the agar disk diffusion method. The β-lactamases production was sought by the liquid acidimetric method. The genes coding for β-lactamases and toxins were identified by PCR method. The results revealed that about 34.95% of the analyzed samples were contaminated by E. coli. Cabbages were the most contaminated by E. coli (28.26%) in dry season. All isolated strains were resistant to amoxicillin. The penicillinase producing E. coli carried bla(TEM) (67.50%), bla(SHV) (10%), and bla(CTX-M) (22.50%) genes. The study revealed that the resistance genes such as SLTI (35.71%), SLTII (35.71%), ETEC (7.15%), and VTEC (21.43%) were carried. Openly to the found results and considering the importance of horticultural products in Beninese food habits, it is important to put several strategies aiming at a sanitary security by surveillance and sensitization of all the actors on the risks of some practices. |
format | Online Article Text |
id | pubmed-4685095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-46850952016-01-14 Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin Moussé, Wassiyath Sina, Haziz Baba-Moussa, Farid Noumavo, Pacôme A. Agbodjato, Nadège A. Adjanohoun, Adolphe Baba-Moussa, Lamine Biomed Res Int Research Article The present study aimed at biochemical and molecular characterization of Escherichia coli strains isolated from horticultural products and irrigation water of Cotonou. The samples were collected from 12 market gardeners of 4 different sites. Rapid' E. coli medium was used for identification of E. coli strains and the antimicrobial susceptibility was performed by the agar disk diffusion method. The β-lactamases production was sought by the liquid acidimetric method. The genes coding for β-lactamases and toxins were identified by PCR method. The results revealed that about 34.95% of the analyzed samples were contaminated by E. coli. Cabbages were the most contaminated by E. coli (28.26%) in dry season. All isolated strains were resistant to amoxicillin. The penicillinase producing E. coli carried bla(TEM) (67.50%), bla(SHV) (10%), and bla(CTX-M) (22.50%) genes. The study revealed that the resistance genes such as SLTI (35.71%), SLTII (35.71%), ETEC (7.15%), and VTEC (21.43%) were carried. Openly to the found results and considering the importance of horticultural products in Beninese food habits, it is important to put several strategies aiming at a sanitary security by surveillance and sensitization of all the actors on the risks of some practices. Hindawi Publishing Corporation 2015 2015-12-07 /pmc/articles/PMC4685095/ /pubmed/26770972 http://dx.doi.org/10.1155/2015/286473 Text en Copyright © 2015 Wassiyath Moussé et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Moussé, Wassiyath Sina, Haziz Baba-Moussa, Farid Noumavo, Pacôme A. Agbodjato, Nadège A. Adjanohoun, Adolphe Baba-Moussa, Lamine Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin |
title | Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin |
title_full | Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin |
title_fullStr | Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin |
title_full_unstemmed | Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin |
title_short | Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin |
title_sort | identification of extended-spectrum β-lactamases escherichia coli strains isolated from market garden products and irrigation water in benin |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685095/ https://www.ncbi.nlm.nih.gov/pubmed/26770972 http://dx.doi.org/10.1155/2015/286473 |
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