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Distribution of Shiga toxin genes subtypes in B(1) phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays

BACKGROUND AND OBJECTIVES: Shiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and contamination via animal origin has been a major public health concern. We compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains of...

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Autores principales: Staji, Hamid, Tonelli, Alfreda, Javaheri-Vayeghan, Abbas, Changizi, Emad, Salimi-Bejestani, Mohammad Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685162/
https://www.ncbi.nlm.nih.gov/pubmed/26697157
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author Staji, Hamid
Tonelli, Alfreda
Javaheri-Vayeghan, Abbas
Changizi, Emad
Salimi-Bejestani, Mohammad Reza
author_facet Staji, Hamid
Tonelli, Alfreda
Javaheri-Vayeghan, Abbas
Changizi, Emad
Salimi-Bejestani, Mohammad Reza
author_sort Staji, Hamid
collection PubMed
description BACKGROUND AND OBJECTIVES: Shiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and contamination via animal origin has been a major public health concern. We compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains of Escherichia coli isolated from feces of diarrheatic calves in Tehran suburb farms. MATERIALS AND METHODS: In this study we screened 140 diarrheatic calves (1–15 days old) for E. coli strains during a 3 months period of time. The isolated strains were grouped into different phylotypes according to the presence of chuA, yjaA and TSPE4.C2 genes. Then, the prevalence of stx gene subtypes was evaluated in the B(1) phylotypes. RESULTS: From diarrheatic calves, 51 bacterial isolates were biochemically identified as E. coli and 31 isolates out of 51 were considered B(1) phylotype using DNA Microarray technology. Of these isolates, 20 contained stx(1)a and stx(1)b and one harbored all mentioned variants of stx genes except stx(2)b(2). CONCLUSION: This study showed that in Tehran suburb, the B(1) phylotype of E. coli is prevalent as a causative agent of diarrhea in calves and the prevalence of stx(1) gene subtypes is dominant in comparison with other subtypes. Considering the possibility that these stx genes can be spread to other strains, bovine E. coli strains are an important source of stx genes for other strains and further study and surveillance seems to be required for the exact identification of virulence profile of E. coli phylotypes in different hosts.
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spelling pubmed-46851622015-12-22 Distribution of Shiga toxin genes subtypes in B(1) phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays Staji, Hamid Tonelli, Alfreda Javaheri-Vayeghan, Abbas Changizi, Emad Salimi-Bejestani, Mohammad Reza Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Shiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and contamination via animal origin has been a major public health concern. We compared the distribution of phylogenetic groups and prevalence of stx gene variants among the pathogenic strains of Escherichia coli isolated from feces of diarrheatic calves in Tehran suburb farms. MATERIALS AND METHODS: In this study we screened 140 diarrheatic calves (1–15 days old) for E. coli strains during a 3 months period of time. The isolated strains were grouped into different phylotypes according to the presence of chuA, yjaA and TSPE4.C2 genes. Then, the prevalence of stx gene subtypes was evaluated in the B(1) phylotypes. RESULTS: From diarrheatic calves, 51 bacterial isolates were biochemically identified as E. coli and 31 isolates out of 51 were considered B(1) phylotype using DNA Microarray technology. Of these isolates, 20 contained stx(1)a and stx(1)b and one harbored all mentioned variants of stx genes except stx(2)b(2). CONCLUSION: This study showed that in Tehran suburb, the B(1) phylotype of E. coli is prevalent as a causative agent of diarrhea in calves and the prevalence of stx(1) gene subtypes is dominant in comparison with other subtypes. Considering the possibility that these stx genes can be spread to other strains, bovine E. coli strains are an important source of stx genes for other strains and further study and surveillance seems to be required for the exact identification of virulence profile of E. coli phylotypes in different hosts. Tehran University of Medical Sciences 2015-08 /pmc/articles/PMC4685162/ /pubmed/26697157 Text en Copyright© 2015 Iranian Neuroscience Society This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Staji, Hamid
Tonelli, Alfreda
Javaheri-Vayeghan, Abbas
Changizi, Emad
Salimi-Bejestani, Mohammad Reza
Distribution of Shiga toxin genes subtypes in B(1) phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays
title Distribution of Shiga toxin genes subtypes in B(1) phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays
title_full Distribution of Shiga toxin genes subtypes in B(1) phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays
title_fullStr Distribution of Shiga toxin genes subtypes in B(1) phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays
title_full_unstemmed Distribution of Shiga toxin genes subtypes in B(1) phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays
title_short Distribution of Shiga toxin genes subtypes in B(1) phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays
title_sort distribution of shiga toxin genes subtypes in b(1) phylotypes of escherichia coli isolated from calves suffering from diarrhea in tehran suburb using dna oligonucleotide arrays
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685162/
https://www.ncbi.nlm.nih.gov/pubmed/26697157
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