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A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos

The aim of this study was to determine the optimum conditions for vitrifying in vitro produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting vitr...

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Autores principales: BARTOLAC, Louise Katherine, LOWE, Jenna Louise, KOUSTAS, George, SJÖBLOM, Cecilia, GRUPEN, Christopher Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685218/
https://www.ncbi.nlm.nih.gov/pubmed/26211782
http://dx.doi.org/10.1262/jrd.2015-065
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author BARTOLAC, Louise Katherine
LOWE, Jenna Louise
KOUSTAS, George
SJÖBLOM, Cecilia
GRUPEN, Christopher Gerald
author_facet BARTOLAC, Louise Katherine
LOWE, Jenna Louise
KOUSTAS, George
SJÖBLOM, Cecilia
GRUPEN, Christopher Gerald
author_sort BARTOLAC, Louise Katherine
collection PubMed
description The aim of this study was to determine the optimum conditions for vitrifying in vitro produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting vitrification. In the next experiment, non-collapsed embryos were vitrified in an open device using either superfine open-pulled straws (SOPS) or the CryoLoop(TM) system, or vitrified in a closed device using either the CryoTip(TM) or Cryo Bio(TM)’s high security vitrification system (HSV). The post-thaw survival of embryos vitrified in the open devices did not differ significantly (SOPS: 37.3%; CryoLoop(TM): 37.3%) nor did the post-thaw survival of embryos vitrified in the closed devices (CryoTip™: 38.5%; HSV: 42.5%). The re-expansion rate of embryos that were collapsed via micro-pipetting (76.0%) did not differ from those that were punctured (75.0%) or collapsed via sucrose (79.6%) when vitrification was not performed. However, embryos collapsed via sucrose solutions (24.5%) and needle puncture (16.0%) prior to vitrification were significantly less likely to survive vitrification than the control (non-collapsed) embryos (53.6%, P < 0.05). The findings show that both open and closed vitrification devices were equally effective for the vitrification of porcine blastocysts. Collapsing blastocysts prior to vitrification did not improve survival, which is inconsistent with the findings of studies in other species. This may be due to the extremely sensitive nature of porcine embryos, and/or the invasiveness of the collapsing procedures.
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spelling pubmed-46852182015-12-22 A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos BARTOLAC, Louise Katherine LOWE, Jenna Louise KOUSTAS, George SJÖBLOM, Cecilia GRUPEN, Christopher Gerald J Reprod Dev Original Article The aim of this study was to determine the optimum conditions for vitrifying in vitro produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting vitrification. In the next experiment, non-collapsed embryos were vitrified in an open device using either superfine open-pulled straws (SOPS) or the CryoLoop(TM) system, or vitrified in a closed device using either the CryoTip(TM) or Cryo Bio(TM)’s high security vitrification system (HSV). The post-thaw survival of embryos vitrified in the open devices did not differ significantly (SOPS: 37.3%; CryoLoop(TM): 37.3%) nor did the post-thaw survival of embryos vitrified in the closed devices (CryoTip™: 38.5%; HSV: 42.5%). The re-expansion rate of embryos that were collapsed via micro-pipetting (76.0%) did not differ from those that were punctured (75.0%) or collapsed via sucrose (79.6%) when vitrification was not performed. However, embryos collapsed via sucrose solutions (24.5%) and needle puncture (16.0%) prior to vitrification were significantly less likely to survive vitrification than the control (non-collapsed) embryos (53.6%, P < 0.05). The findings show that both open and closed vitrification devices were equally effective for the vitrification of porcine blastocysts. Collapsing blastocysts prior to vitrification did not improve survival, which is inconsistent with the findings of studies in other species. This may be due to the extremely sensitive nature of porcine embryos, and/or the invasiveness of the collapsing procedures. The Society for Reproduction and Development 2015-07-23 2015-12 /pmc/articles/PMC4685218/ /pubmed/26211782 http://dx.doi.org/10.1262/jrd.2015-065 Text en ©2015 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Original Article
BARTOLAC, Louise Katherine
LOWE, Jenna Louise
KOUSTAS, George
SJÖBLOM, Cecilia
GRUPEN, Christopher Gerald
A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
title A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
title_full A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
title_fullStr A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
title_full_unstemmed A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
title_short A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
title_sort comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685218/
https://www.ncbi.nlm.nih.gov/pubmed/26211782
http://dx.doi.org/10.1262/jrd.2015-065
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