Cargando…
A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
The aim of this study was to determine the optimum conditions for vitrifying in vitro produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting vitr...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685218/ https://www.ncbi.nlm.nih.gov/pubmed/26211782 http://dx.doi.org/10.1262/jrd.2015-065 |
_version_ | 1782406273522728960 |
---|---|
author | BARTOLAC, Louise Katherine LOWE, Jenna Louise KOUSTAS, George SJÖBLOM, Cecilia GRUPEN, Christopher Gerald |
author_facet | BARTOLAC, Louise Katherine LOWE, Jenna Louise KOUSTAS, George SJÖBLOM, Cecilia GRUPEN, Christopher Gerald |
author_sort | BARTOLAC, Louise Katherine |
collection | PubMed |
description | The aim of this study was to determine the optimum conditions for vitrifying in vitro produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting vitrification. In the next experiment, non-collapsed embryos were vitrified in an open device using either superfine open-pulled straws (SOPS) or the CryoLoop(TM) system, or vitrified in a closed device using either the CryoTip(TM) or Cryo Bio(TM)’s high security vitrification system (HSV). The post-thaw survival of embryos vitrified in the open devices did not differ significantly (SOPS: 37.3%; CryoLoop(TM): 37.3%) nor did the post-thaw survival of embryos vitrified in the closed devices (CryoTip™: 38.5%; HSV: 42.5%). The re-expansion rate of embryos that were collapsed via micro-pipetting (76.0%) did not differ from those that were punctured (75.0%) or collapsed via sucrose (79.6%) when vitrification was not performed. However, embryos collapsed via sucrose solutions (24.5%) and needle puncture (16.0%) prior to vitrification were significantly less likely to survive vitrification than the control (non-collapsed) embryos (53.6%, P < 0.05). The findings show that both open and closed vitrification devices were equally effective for the vitrification of porcine blastocysts. Collapsing blastocysts prior to vitrification did not improve survival, which is inconsistent with the findings of studies in other species. This may be due to the extremely sensitive nature of porcine embryos, and/or the invasiveness of the collapsing procedures. |
format | Online Article Text |
id | pubmed-4685218 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-46852182015-12-22 A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos BARTOLAC, Louise Katherine LOWE, Jenna Louise KOUSTAS, George SJÖBLOM, Cecilia GRUPEN, Christopher Gerald J Reprod Dev Original Article The aim of this study was to determine the optimum conditions for vitrifying in vitro produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting vitrification. In the next experiment, non-collapsed embryos were vitrified in an open device using either superfine open-pulled straws (SOPS) or the CryoLoop(TM) system, or vitrified in a closed device using either the CryoTip(TM) or Cryo Bio(TM)’s high security vitrification system (HSV). The post-thaw survival of embryos vitrified in the open devices did not differ significantly (SOPS: 37.3%; CryoLoop(TM): 37.3%) nor did the post-thaw survival of embryos vitrified in the closed devices (CryoTip™: 38.5%; HSV: 42.5%). The re-expansion rate of embryos that were collapsed via micro-pipetting (76.0%) did not differ from those that were punctured (75.0%) or collapsed via sucrose (79.6%) when vitrification was not performed. However, embryos collapsed via sucrose solutions (24.5%) and needle puncture (16.0%) prior to vitrification were significantly less likely to survive vitrification than the control (non-collapsed) embryos (53.6%, P < 0.05). The findings show that both open and closed vitrification devices were equally effective for the vitrification of porcine blastocysts. Collapsing blastocysts prior to vitrification did not improve survival, which is inconsistent with the findings of studies in other species. This may be due to the extremely sensitive nature of porcine embryos, and/or the invasiveness of the collapsing procedures. The Society for Reproduction and Development 2015-07-23 2015-12 /pmc/articles/PMC4685218/ /pubmed/26211782 http://dx.doi.org/10.1262/jrd.2015-065 Text en ©2015 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Article BARTOLAC, Louise Katherine LOWE, Jenna Louise KOUSTAS, George SJÖBLOM, Cecilia GRUPEN, Christopher Gerald A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos |
title | A comparison of different vitrification devices and the effect of blastocoele collapse on the
cryosurvival of in vitro produced porcine embryos |
title_full | A comparison of different vitrification devices and the effect of blastocoele collapse on the
cryosurvival of in vitro produced porcine embryos |
title_fullStr | A comparison of different vitrification devices and the effect of blastocoele collapse on the
cryosurvival of in vitro produced porcine embryos |
title_full_unstemmed | A comparison of different vitrification devices and the effect of blastocoele collapse on the
cryosurvival of in vitro produced porcine embryos |
title_short | A comparison of different vitrification devices and the effect of blastocoele collapse on the
cryosurvival of in vitro produced porcine embryos |
title_sort | comparison of different vitrification devices and the effect of blastocoele collapse on the
cryosurvival of in vitro produced porcine embryos |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685218/ https://www.ncbi.nlm.nih.gov/pubmed/26211782 http://dx.doi.org/10.1262/jrd.2015-065 |
work_keys_str_mv | AT bartolaclouisekatherine acomparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos AT lowejennalouise acomparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos AT koustasgeorge acomparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos AT sjoblomcecilia acomparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos AT grupenchristophergerald acomparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos AT bartolaclouisekatherine comparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos AT lowejennalouise comparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos AT koustasgeorge comparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos AT sjoblomcecilia comparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos AT grupenchristophergerald comparisonofdifferentvitrificationdevicesandtheeffectofblastocoelecollapseonthecryosurvivalofinvitroproducedporcineembryos |