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Optimization of cryoprotectant treatment for the vitrification of immature cumulus-enclosed porcine oocytes: comparison of sugars, combinations of permeating cryoprotectants and equilibration regimens

Our aim was to optimize the cryoprotectant treatment for the preservation of immature porcine cumulus-oocyte complexes (COCs) by solid surface vitrification. In each experiment, the vitrification solution consisted of 50 mg/ml polyvinyl pyrrolidone, 0.3 M of the actual sugar and in total 35% (v/v) o...

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Detalles Bibliográficos
Autores principales:	SOMFAI, Tamás, MEN, Nguyen Thi, NOGUCHI, Junko, KANEKO, Hiroyuki, KASHIWAZAKI, Naomi, KIKUCHI, Kazuhiro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Society for Reproduction and Development 2015
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685224/ https://www.ncbi.nlm.nih.gov/pubmed/26411536 http://dx.doi.org/10.1262/jrd.2015-089

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