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The cytotoxic T cell proteome and its shaping by mammalian Target of Rapamycin
High-resolution mass spectrometry maps the cytotoxic T lymphocyte (CTL) proteome and the impact of mammalian target of rapamycin complex 1 (mTORC1) on CTLs. The CTL proteome was dominated by metabolic regulators and granzymes and mTORC1 selectively repressed and promoted expression of subset of CTL...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685757/ https://www.ncbi.nlm.nih.gov/pubmed/26551880 http://dx.doi.org/10.1038/ni.3314 |
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author | Hukelmann, Jens L. Anderson, Karen E. Sinclair, Linda V. Grzes, Katarzyna M. Murillo, Alejandro Brenes Hawkins, Phillip T. Stephens, Len R. Lamond, Angus I. Cantrell, Doreen A. |
author_facet | Hukelmann, Jens L. Anderson, Karen E. Sinclair, Linda V. Grzes, Katarzyna M. Murillo, Alejandro Brenes Hawkins, Phillip T. Stephens, Len R. Lamond, Angus I. Cantrell, Doreen A. |
author_sort | Hukelmann, Jens L. |
collection | PubMed |
description | High-resolution mass spectrometry maps the cytotoxic T lymphocyte (CTL) proteome and the impact of mammalian target of rapamycin complex 1 (mTORC1) on CTLs. The CTL proteome was dominated by metabolic regulators and granzymes and mTORC1 selectively repressed and promoted expression of subset of CTL proteins (~10%). These included key CTL effector molecules, signaling proteins and a subset of metabolic enzymes. Proteomic data highlighted the potential for mTORC1 negative control of phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P(3)) production in CTL. mTORC1 was shown to repress PtdIns(3,4,5)P(3) production and to determine the mTORC2 requirement for activation of the kinase Akt. Unbiased proteomic analysis thus provides a comprehensive understanding of CTL identity and mTORC1 control of CTL function. |
format | Online Article Text |
id | pubmed-4685757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-46857572016-05-18 The cytotoxic T cell proteome and its shaping by mammalian Target of Rapamycin Hukelmann, Jens L. Anderson, Karen E. Sinclair, Linda V. Grzes, Katarzyna M. Murillo, Alejandro Brenes Hawkins, Phillip T. Stephens, Len R. Lamond, Angus I. Cantrell, Doreen A. Nat Immunol Article High-resolution mass spectrometry maps the cytotoxic T lymphocyte (CTL) proteome and the impact of mammalian target of rapamycin complex 1 (mTORC1) on CTLs. The CTL proteome was dominated by metabolic regulators and granzymes and mTORC1 selectively repressed and promoted expression of subset of CTL proteins (~10%). These included key CTL effector molecules, signaling proteins and a subset of metabolic enzymes. Proteomic data highlighted the potential for mTORC1 negative control of phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P(3)) production in CTL. mTORC1 was shown to repress PtdIns(3,4,5)P(3) production and to determine the mTORC2 requirement for activation of the kinase Akt. Unbiased proteomic analysis thus provides a comprehensive understanding of CTL identity and mTORC1 control of CTL function. 2015-11-09 2016-01 /pmc/articles/PMC4685757/ /pubmed/26551880 http://dx.doi.org/10.1038/ni.3314 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Hukelmann, Jens L. Anderson, Karen E. Sinclair, Linda V. Grzes, Katarzyna M. Murillo, Alejandro Brenes Hawkins, Phillip T. Stephens, Len R. Lamond, Angus I. Cantrell, Doreen A. The cytotoxic T cell proteome and its shaping by mammalian Target of Rapamycin |
title | The cytotoxic T cell proteome and its shaping by mammalian Target of Rapamycin |
title_full | The cytotoxic T cell proteome and its shaping by mammalian Target of Rapamycin |
title_fullStr | The cytotoxic T cell proteome and its shaping by mammalian Target of Rapamycin |
title_full_unstemmed | The cytotoxic T cell proteome and its shaping by mammalian Target of Rapamycin |
title_short | The cytotoxic T cell proteome and its shaping by mammalian Target of Rapamycin |
title_sort | cytotoxic t cell proteome and its shaping by mammalian target of rapamycin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685757/ https://www.ncbi.nlm.nih.gov/pubmed/26551880 http://dx.doi.org/10.1038/ni.3314 |
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