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Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles
Silver nanoparticles offer a possible means of fighting antibacterial resistance. Most of their antibacterial properties are attributed to their silver ions. In the present work, we study the actions of positively charged silver nanoparticles against both methicillin-sensitive Staphylococcus aureus...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Beilstein-Institut
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685924/ https://www.ncbi.nlm.nih.gov/pubmed/26734530 http://dx.doi.org/10.3762/bjnano.6.246 |
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author | Romero-Urbina, Dulce G Lara, Humberto H Velázquez-Salazar, J Jesús Arellano-Jiménez, M Josefina Larios, Eduardo Srinivasan, Anand Lopez-Ribot, Jose L Yacamán, Miguel José |
author_facet | Romero-Urbina, Dulce G Lara, Humberto H Velázquez-Salazar, J Jesús Arellano-Jiménez, M Josefina Larios, Eduardo Srinivasan, Anand Lopez-Ribot, Jose L Yacamán, Miguel José |
author_sort | Romero-Urbina, Dulce G |
collection | PubMed |
description | Silver nanoparticles offer a possible means of fighting antibacterial resistance. Most of their antibacterial properties are attributed to their silver ions. In the present work, we study the actions of positively charged silver nanoparticles against both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. We use aberration-corrected transmission electron microscopy to examine the bactericidal effects of silver nanoparticles and the ultrastructural changes in bacteria that are induced by silver nanoparticles. The study revealed that our 1 nm average size silver nanoparticles induced thinning and permeabilization of the cell wall, destabilization of the peptidoglycan layer, and subsequent leakage of intracellular content, causing bacterial cell lysis. We hypothesize that positively charged silver nanoparticles bind to the negatively charged polyanionic backbones of teichoic acids and the related cell wall glycopolymers of bacteria as a first target, consequently stressing the structure and permeability of the cell wall. This hypothesis provides a major mechanism to explain the antibacterial effects of silver nanoparticles on Staphylococcus aureus. Future research should focus on defining the related molecular mechanisms and their importance to the antimicrobial activity of silver nanoparticles. |
format | Online Article Text |
id | pubmed-4685924 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Beilstein-Institut |
record_format | MEDLINE/PubMed |
spelling | pubmed-46859242016-01-05 Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles Romero-Urbina, Dulce G Lara, Humberto H Velázquez-Salazar, J Jesús Arellano-Jiménez, M Josefina Larios, Eduardo Srinivasan, Anand Lopez-Ribot, Jose L Yacamán, Miguel José Beilstein J Nanotechnol Full Research Paper Silver nanoparticles offer a possible means of fighting antibacterial resistance. Most of their antibacterial properties are attributed to their silver ions. In the present work, we study the actions of positively charged silver nanoparticles against both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. We use aberration-corrected transmission electron microscopy to examine the bactericidal effects of silver nanoparticles and the ultrastructural changes in bacteria that are induced by silver nanoparticles. The study revealed that our 1 nm average size silver nanoparticles induced thinning and permeabilization of the cell wall, destabilization of the peptidoglycan layer, and subsequent leakage of intracellular content, causing bacterial cell lysis. We hypothesize that positively charged silver nanoparticles bind to the negatively charged polyanionic backbones of teichoic acids and the related cell wall glycopolymers of bacteria as a first target, consequently stressing the structure and permeability of the cell wall. This hypothesis provides a major mechanism to explain the antibacterial effects of silver nanoparticles on Staphylococcus aureus. Future research should focus on defining the related molecular mechanisms and their importance to the antimicrobial activity of silver nanoparticles. Beilstein-Institut 2015-12-15 /pmc/articles/PMC4685924/ /pubmed/26734530 http://dx.doi.org/10.3762/bjnano.6.246 Text en Copyright © 2015, Romero-Urbina et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjnano/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms) |
spellingShingle | Full Research Paper Romero-Urbina, Dulce G Lara, Humberto H Velázquez-Salazar, J Jesús Arellano-Jiménez, M Josefina Larios, Eduardo Srinivasan, Anand Lopez-Ribot, Jose L Yacamán, Miguel José Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles |
title | Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles |
title_full | Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles |
title_fullStr | Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles |
title_full_unstemmed | Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles |
title_short | Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles |
title_sort | ultrastructural changes in methicillin-resistant staphylococcus aureus induced by positively charged silver nanoparticles |
topic | Full Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685924/ https://www.ncbi.nlm.nih.gov/pubmed/26734530 http://dx.doi.org/10.3762/bjnano.6.246 |
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