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MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4

OBJECTIVE(S): MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression. They have important roles in kidney development, homeostasis and disease, and participate in the onset and progression of tubulointerstitial sclerosis and end-stage glomerular lesions that occur in var...

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Autores principales: Zhang, Hongxia, Zhang, Xiaoning, Yuan, Xiaoying, Wang, Linna, Xiao, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686573/
https://www.ncbi.nlm.nih.gov/pubmed/26730338
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author Zhang, Hongxia
Zhang, Xiaoning
Yuan, Xiaoying
Wang, Linna
Xiao, Ying
author_facet Zhang, Hongxia
Zhang, Xiaoning
Yuan, Xiaoying
Wang, Linna
Xiao, Ying
author_sort Zhang, Hongxia
collection PubMed
description OBJECTIVE(S): MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression. They have important roles in kidney development, homeostasis and disease, and participate in the onset and progression of tubulointerstitial sclerosis and end-stage glomerular lesions that occur in various forms of chronic kidney disease (CKD). In the present study, we elucidated the role of microRNA 205 (miR-205) in cisplatin-induced renal cell apoptosis and explored the molecular mechanisms. MATERIALS AND METHODS: The chronic interstitial nephropathy rat model was induced, and the miRNA expression profile in the kidney cells from rats with CKD was screened. Cisplatin-induced apoptosis in normal renal HK-2 cells was evaluated using flow cytometry, and regulation of miR-205 on target gene was validated using luciferase assay, western blot and real time PCR assays. RESULTS: We found that miR-205 expression was significantly decreased in the cells from kidney of CKD rat (P<0.01). Our data showed that when miR-205 was overexpressed or silenced using the mimic or inhibitor, the percentages of apoptotic cells were suppressed or increased significantly (P<0.05), respectively. Moreover, we have identified CMTM4 gene, which is involved in cell proliferation and apoptosis, as a novel target for miR-205. In addition, miR-205 could inhibit apoptosis by binding to the 3’UTR of CMTM4 mRNA and inhibiting its transcriptional activity. CONCLUSION: This study elucidated that miR-205 plays an important role in the regulation of apoptosis in renal cells, suggesting a potential therapeutic target to hinder CKD development.
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spelling pubmed-46865732016-01-04 MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4 Zhang, Hongxia Zhang, Xiaoning Yuan, Xiaoying Wang, Linna Xiao, Ying Iran J Basic Med Sci Original Article OBJECTIVE(S): MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression. They have important roles in kidney development, homeostasis and disease, and participate in the onset and progression of tubulointerstitial sclerosis and end-stage glomerular lesions that occur in various forms of chronic kidney disease (CKD). In the present study, we elucidated the role of microRNA 205 (miR-205) in cisplatin-induced renal cell apoptosis and explored the molecular mechanisms. MATERIALS AND METHODS: The chronic interstitial nephropathy rat model was induced, and the miRNA expression profile in the kidney cells from rats with CKD was screened. Cisplatin-induced apoptosis in normal renal HK-2 cells was evaluated using flow cytometry, and regulation of miR-205 on target gene was validated using luciferase assay, western blot and real time PCR assays. RESULTS: We found that miR-205 expression was significantly decreased in the cells from kidney of CKD rat (P<0.01). Our data showed that when miR-205 was overexpressed or silenced using the mimic or inhibitor, the percentages of apoptotic cells were suppressed or increased significantly (P<0.05), respectively. Moreover, we have identified CMTM4 gene, which is involved in cell proliferation and apoptosis, as a novel target for miR-205. In addition, miR-205 could inhibit apoptosis by binding to the 3’UTR of CMTM4 mRNA and inhibiting its transcriptional activity. CONCLUSION: This study elucidated that miR-205 plays an important role in the regulation of apoptosis in renal cells, suggesting a potential therapeutic target to hinder CKD development. Mashhad University of Medical Sciences 2015-10 /pmc/articles/PMC4686573/ /pubmed/26730338 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zhang, Hongxia
Zhang, Xiaoning
Yuan, Xiaoying
Wang, Linna
Xiao, Ying
MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4
title MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4
title_full MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4
title_fullStr MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4
title_full_unstemmed MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4
title_short MicroRNA-205 inhibits renal cells apoptosis via targeting CMTM4
title_sort microrna-205 inhibits renal cells apoptosis via targeting cmtm4
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686573/
https://www.ncbi.nlm.nih.gov/pubmed/26730338
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