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Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development

Streptomyces coelicolor is a model actinomycete that is well known for the diversity of its secondary metabolism and its complex life cycle. As a soil inhabitant, it is exposed to heterogeneous and frequently changing environmental circumstances. In the present work, we studied the effect of diverse...

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Autores principales: Sandoval-Calderón, Mario, Nguyen, Don D., Kapono, Clifford A., Herron, Paul, Dorrestein, Pieter C., Sohlenkamp, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686642/
https://www.ncbi.nlm.nih.gov/pubmed/26733994
http://dx.doi.org/10.3389/fmicb.2015.01465
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author Sandoval-Calderón, Mario
Nguyen, Don D.
Kapono, Clifford A.
Herron, Paul
Dorrestein, Pieter C.
Sohlenkamp, Christian
author_facet Sandoval-Calderón, Mario
Nguyen, Don D.
Kapono, Clifford A.
Herron, Paul
Dorrestein, Pieter C.
Sohlenkamp, Christian
author_sort Sandoval-Calderón, Mario
collection PubMed
description Streptomyces coelicolor is a model actinomycete that is well known for the diversity of its secondary metabolism and its complex life cycle. As a soil inhabitant, it is exposed to heterogeneous and frequently changing environmental circumstances. In the present work, we studied the effect of diverse growth conditions and phosphate depletion on its lipid profile and the relationship between membrane lipid composition and development in S. coelicolor. The lipid profile from cultures grown on solid media, which is closer to the natural habitat of this microorganism, does not resemble the previously reported lipid composition from liquid grown cultures of S. coelicolor. Wide variations were also observed across different media, growth phases, and developmental stages indicating active membrane remodeling. Ornithine lipids (OL) are phosphorus-free polar lipids that were accumulated mainly during sporulation stages, but were also major components of the membrane under phosphorus limitation. In contrast, phosphatidylethanolamine, which had been reported as one of the major polar lipids in the genus Streptomyces, is almost absent under these conditions. We identified one of the genes responsible for the synthesis of OL (SCO0921) and found that its inactivation causes the absence of OL, precocious morphological development and actinorhodin production. Our observations indicate a remarkable plasticity of the membrane composition in this bacterial species, reveal a higher metabolic complexity than expected, and suggest a relationship between cytoplasmic membrane components and the differentiation programs in S. coelicolor.
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spelling pubmed-46866422016-01-05 Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development Sandoval-Calderón, Mario Nguyen, Don D. Kapono, Clifford A. Herron, Paul Dorrestein, Pieter C. Sohlenkamp, Christian Front Microbiol Microbiology Streptomyces coelicolor is a model actinomycete that is well known for the diversity of its secondary metabolism and its complex life cycle. As a soil inhabitant, it is exposed to heterogeneous and frequently changing environmental circumstances. In the present work, we studied the effect of diverse growth conditions and phosphate depletion on its lipid profile and the relationship between membrane lipid composition and development in S. coelicolor. The lipid profile from cultures grown on solid media, which is closer to the natural habitat of this microorganism, does not resemble the previously reported lipid composition from liquid grown cultures of S. coelicolor. Wide variations were also observed across different media, growth phases, and developmental stages indicating active membrane remodeling. Ornithine lipids (OL) are phosphorus-free polar lipids that were accumulated mainly during sporulation stages, but were also major components of the membrane under phosphorus limitation. In contrast, phosphatidylethanolamine, which had been reported as one of the major polar lipids in the genus Streptomyces, is almost absent under these conditions. We identified one of the genes responsible for the synthesis of OL (SCO0921) and found that its inactivation causes the absence of OL, precocious morphological development and actinorhodin production. Our observations indicate a remarkable plasticity of the membrane composition in this bacterial species, reveal a higher metabolic complexity than expected, and suggest a relationship between cytoplasmic membrane components and the differentiation programs in S. coelicolor. Frontiers Media S.A. 2015-12-22 /pmc/articles/PMC4686642/ /pubmed/26733994 http://dx.doi.org/10.3389/fmicb.2015.01465 Text en Copyright © 2015 Sandoval-Calderón, Nguyen, Kapono, Herron, Dorrestein and Sohlenkamp. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sandoval-Calderón, Mario
Nguyen, Don D.
Kapono, Clifford A.
Herron, Paul
Dorrestein, Pieter C.
Sohlenkamp, Christian
Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development
title Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development
title_full Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development
title_fullStr Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development
title_full_unstemmed Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development
title_short Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development
title_sort plasticity of streptomyces coelicolor membrane composition under different growth conditions and during development
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686642/
https://www.ncbi.nlm.nih.gov/pubmed/26733994
http://dx.doi.org/10.3389/fmicb.2015.01465
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