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Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development
Streptomyces coelicolor is a model actinomycete that is well known for the diversity of its secondary metabolism and its complex life cycle. As a soil inhabitant, it is exposed to heterogeneous and frequently changing environmental circumstances. In the present work, we studied the effect of diverse...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686642/ https://www.ncbi.nlm.nih.gov/pubmed/26733994 http://dx.doi.org/10.3389/fmicb.2015.01465 |
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author | Sandoval-Calderón, Mario Nguyen, Don D. Kapono, Clifford A. Herron, Paul Dorrestein, Pieter C. Sohlenkamp, Christian |
author_facet | Sandoval-Calderón, Mario Nguyen, Don D. Kapono, Clifford A. Herron, Paul Dorrestein, Pieter C. Sohlenkamp, Christian |
author_sort | Sandoval-Calderón, Mario |
collection | PubMed |
description | Streptomyces coelicolor is a model actinomycete that is well known for the diversity of its secondary metabolism and its complex life cycle. As a soil inhabitant, it is exposed to heterogeneous and frequently changing environmental circumstances. In the present work, we studied the effect of diverse growth conditions and phosphate depletion on its lipid profile and the relationship between membrane lipid composition and development in S. coelicolor. The lipid profile from cultures grown on solid media, which is closer to the natural habitat of this microorganism, does not resemble the previously reported lipid composition from liquid grown cultures of S. coelicolor. Wide variations were also observed across different media, growth phases, and developmental stages indicating active membrane remodeling. Ornithine lipids (OL) are phosphorus-free polar lipids that were accumulated mainly during sporulation stages, but were also major components of the membrane under phosphorus limitation. In contrast, phosphatidylethanolamine, which had been reported as one of the major polar lipids in the genus Streptomyces, is almost absent under these conditions. We identified one of the genes responsible for the synthesis of OL (SCO0921) and found that its inactivation causes the absence of OL, precocious morphological development and actinorhodin production. Our observations indicate a remarkable plasticity of the membrane composition in this bacterial species, reveal a higher metabolic complexity than expected, and suggest a relationship between cytoplasmic membrane components and the differentiation programs in S. coelicolor. |
format | Online Article Text |
id | pubmed-4686642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46866422016-01-05 Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development Sandoval-Calderón, Mario Nguyen, Don D. Kapono, Clifford A. Herron, Paul Dorrestein, Pieter C. Sohlenkamp, Christian Front Microbiol Microbiology Streptomyces coelicolor is a model actinomycete that is well known for the diversity of its secondary metabolism and its complex life cycle. As a soil inhabitant, it is exposed to heterogeneous and frequently changing environmental circumstances. In the present work, we studied the effect of diverse growth conditions and phosphate depletion on its lipid profile and the relationship between membrane lipid composition and development in S. coelicolor. The lipid profile from cultures grown on solid media, which is closer to the natural habitat of this microorganism, does not resemble the previously reported lipid composition from liquid grown cultures of S. coelicolor. Wide variations were also observed across different media, growth phases, and developmental stages indicating active membrane remodeling. Ornithine lipids (OL) are phosphorus-free polar lipids that were accumulated mainly during sporulation stages, but were also major components of the membrane under phosphorus limitation. In contrast, phosphatidylethanolamine, which had been reported as one of the major polar lipids in the genus Streptomyces, is almost absent under these conditions. We identified one of the genes responsible for the synthesis of OL (SCO0921) and found that its inactivation causes the absence of OL, precocious morphological development and actinorhodin production. Our observations indicate a remarkable plasticity of the membrane composition in this bacterial species, reveal a higher metabolic complexity than expected, and suggest a relationship between cytoplasmic membrane components and the differentiation programs in S. coelicolor. Frontiers Media S.A. 2015-12-22 /pmc/articles/PMC4686642/ /pubmed/26733994 http://dx.doi.org/10.3389/fmicb.2015.01465 Text en Copyright © 2015 Sandoval-Calderón, Nguyen, Kapono, Herron, Dorrestein and Sohlenkamp. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Sandoval-Calderón, Mario Nguyen, Don D. Kapono, Clifford A. Herron, Paul Dorrestein, Pieter C. Sohlenkamp, Christian Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development |
title | Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development |
title_full | Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development |
title_fullStr | Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development |
title_full_unstemmed | Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development |
title_short | Plasticity of Streptomyces coelicolor Membrane Composition Under Different Growth Conditions and During Development |
title_sort | plasticity of streptomyces coelicolor membrane composition under different growth conditions and during development |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686642/ https://www.ncbi.nlm.nih.gov/pubmed/26733994 http://dx.doi.org/10.3389/fmicb.2015.01465 |
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