3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo

We report a method to facilitate single cell, image-guided experiments including in vivo electrophysiology and electroporation. Our method combines 3D image data acquisition, visualization and on-line image analysis with precise control of physical probes such as electrophysiology microelectrodes in...

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Detalles Bibliográficos
Autores principales: Long, Brian, Li, Lu, Knoblich, Ulf, Zeng, Hongkui, Peng, Hanchuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686883/
https://www.ncbi.nlm.nih.gov/pubmed/26689553
http://dx.doi.org/10.1038/srep18426
Descripción
Sumario:We report a method to facilitate single cell, image-guided experiments including in vivo electrophysiology and electroporation. Our method combines 3D image data acquisition, visualization and on-line image analysis with precise control of physical probes such as electrophysiology microelectrodes in brain tissue in vivo. Adaptive pipette positioning provides a platform for future advances in automated, single cell in vivo experiments.

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