3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo

We report a method to facilitate single cell, image-guided experiments including in vivo electrophysiology and electroporation. Our method combines 3D image data acquisition, visualization and on-line image analysis with precise control of physical probes such as electrophysiology microelectrodes in...

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Detalles Bibliográficos
Autores principales: Long, Brian, Li, Lu, Knoblich, Ulf, Zeng, Hongkui, Peng, Hanchuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686883/
https://www.ncbi.nlm.nih.gov/pubmed/26689553
http://dx.doi.org/10.1038/srep18426
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author Long, Brian
Li, Lu
Knoblich, Ulf
Zeng, Hongkui
Peng, Hanchuan
author_facet Long, Brian
Li, Lu
Knoblich, Ulf
Zeng, Hongkui
Peng, Hanchuan
author_sort Long, Brian
collection PubMed
description We report a method to facilitate single cell, image-guided experiments including in vivo electrophysiology and electroporation. Our method combines 3D image data acquisition, visualization and on-line image analysis with precise control of physical probes such as electrophysiology microelectrodes in brain tissue in vivo. Adaptive pipette positioning provides a platform for future advances in automated, single cell in vivo experiments.
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spelling pubmed-46868832015-12-31 3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo Long, Brian Li, Lu Knoblich, Ulf Zeng, Hongkui Peng, Hanchuan Sci Rep Article We report a method to facilitate single cell, image-guided experiments including in vivo electrophysiology and electroporation. Our method combines 3D image data acquisition, visualization and on-line image analysis with precise control of physical probes such as electrophysiology microelectrodes in brain tissue in vivo. Adaptive pipette positioning provides a platform for future advances in automated, single cell in vivo experiments. Nature Publishing Group 2015-12-22 /pmc/articles/PMC4686883/ /pubmed/26689553 http://dx.doi.org/10.1038/srep18426 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Long, Brian
Li, Lu
Knoblich, Ulf
Zeng, Hongkui
Peng, Hanchuan
3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo
title 3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo
title_full 3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo
title_fullStr 3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo
title_full_unstemmed 3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo
title_short 3D Image-Guided Automatic Pipette Positioning for Single Cell Experiments in vivo
title_sort 3d image-guided automatic pipette positioning for single cell experiments in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686883/
https://www.ncbi.nlm.nih.gov/pubmed/26689553
http://dx.doi.org/10.1038/srep18426
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