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Complementary annealing mediated by exonuclease: a method for seamless cloning and conditioning site-directed mutagenesis
Traditional cut-paste DNA cloning is often limited by the availability of restriction enzyme sites. Here, we described the complementary annealing mediated by exonuclease (CAME), in which the insert or vector fragment is amplified to carry sequences complementary to the other, and both fragments are...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Taylor & Francis
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686905/ https://www.ncbi.nlm.nih.gov/pubmed/26740790 http://dx.doi.org/10.1080/13102818.2014.988094 |