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Investigation of single nucleotide polymorphisms based on the intronic sequences of the propylene alcohol dehydrogenase gene in Chinese tobacco genotypes
A pair of primers was designed to amplify the propylene alcohol dehydrogenase gene sequence based on the cDNA sequence of the tobacco allyl-alcohol dehydrogenase gene. All introns were sequenced using traditional polymerase chain reaction (PCR) methods and T-A cloning. The sequences from common toba...
Autores principales: | Wei, Ji-Cheng, Qiu, En-Jian, Guo, Hui-Yan, Hao, Ai-Ping, Chen, Rong-Ping |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686925/ https://www.ncbi.nlm.nih.gov/pubmed/26740754 http://dx.doi.org/10.1080/13102818.2014.907651 |
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