Cargando…
Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum
Viruses are considered key players in phytoplankton population control in oceans. However, mechanisms that control viral gene expression in prominent microalgae such as diatoms remain largely unknown. In this study, potential promoter regions isolated from several marine diatom-infecting viruses (DI...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686930/ https://www.ncbi.nlm.nih.gov/pubmed/26692124 http://dx.doi.org/10.1038/srep18708 |
_version_ | 1782406528021561344 |
---|---|
author | Kadono, Takashi Miyagawa-Yamaguchi, Arisa Kira, Nozomu Tomaru, Yuji Okami, Takuma Yoshimatsu, Takamichi Hou, Liyuan Ohama, Takeshi Fukunaga, Kazunari Okauchi, Masanori Yamaguchi, Haruo Ohnishi, Kohei Falciatore, Angela Adachi, Masao |
author_facet | Kadono, Takashi Miyagawa-Yamaguchi, Arisa Kira, Nozomu Tomaru, Yuji Okami, Takuma Yoshimatsu, Takamichi Hou, Liyuan Ohama, Takeshi Fukunaga, Kazunari Okauchi, Masanori Yamaguchi, Haruo Ohnishi, Kohei Falciatore, Angela Adachi, Masao |
author_sort | Kadono, Takashi |
collection | PubMed |
description | Viruses are considered key players in phytoplankton population control in oceans. However, mechanisms that control viral gene expression in prominent microalgae such as diatoms remain largely unknown. In this study, potential promoter regions isolated from several marine diatom-infecting viruses (DIVs) were linked to the egfp reporter gene and transformed into the Pennales diatom Phaeodactylum tricornutum. We analysed their activity in cells grown under different conditions. Compared to diatom endogenous promoters, novel DIV promoter (ClP1) mediated a significantly higher degree of reporter transcription and translation. Stable expression levels were observed in transformants grown under both light and dark conditions, and high levels of expression were reported in cells in the stationary phase compared to the exponential phase of growth. Conserved motifs in the sequence of DIV promoters were also found. These results allow the identification of novel regulatory regions that drive DIV gene expression and further examinations of the mechanisms that control virus-mediated bloom control in diatoms. Moreover, the identified ClP1 promoter can serve as a novel tool for metabolic engineering of diatoms. This is the first report describing a promoter of DIVs that may be of use in basic and applied diatom research. |
format | Online Article Text |
id | pubmed-4686930 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46869302015-12-31 Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum Kadono, Takashi Miyagawa-Yamaguchi, Arisa Kira, Nozomu Tomaru, Yuji Okami, Takuma Yoshimatsu, Takamichi Hou, Liyuan Ohama, Takeshi Fukunaga, Kazunari Okauchi, Masanori Yamaguchi, Haruo Ohnishi, Kohei Falciatore, Angela Adachi, Masao Sci Rep Article Viruses are considered key players in phytoplankton population control in oceans. However, mechanisms that control viral gene expression in prominent microalgae such as diatoms remain largely unknown. In this study, potential promoter regions isolated from several marine diatom-infecting viruses (DIVs) were linked to the egfp reporter gene and transformed into the Pennales diatom Phaeodactylum tricornutum. We analysed their activity in cells grown under different conditions. Compared to diatom endogenous promoters, novel DIV promoter (ClP1) mediated a significantly higher degree of reporter transcription and translation. Stable expression levels were observed in transformants grown under both light and dark conditions, and high levels of expression were reported in cells in the stationary phase compared to the exponential phase of growth. Conserved motifs in the sequence of DIV promoters were also found. These results allow the identification of novel regulatory regions that drive DIV gene expression and further examinations of the mechanisms that control virus-mediated bloom control in diatoms. Moreover, the identified ClP1 promoter can serve as a novel tool for metabolic engineering of diatoms. This is the first report describing a promoter of DIVs that may be of use in basic and applied diatom research. Nature Publishing Group 2015-12-22 /pmc/articles/PMC4686930/ /pubmed/26692124 http://dx.doi.org/10.1038/srep18708 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kadono, Takashi Miyagawa-Yamaguchi, Arisa Kira, Nozomu Tomaru, Yuji Okami, Takuma Yoshimatsu, Takamichi Hou, Liyuan Ohama, Takeshi Fukunaga, Kazunari Okauchi, Masanori Yamaguchi, Haruo Ohnishi, Kohei Falciatore, Angela Adachi, Masao Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum |
title | Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum |
title_full | Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum |
title_fullStr | Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum |
title_full_unstemmed | Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum |
title_short | Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum |
title_sort | characterization of marine diatom-infecting virus promoters in the model diatom phaeodactylum tricornutum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686930/ https://www.ncbi.nlm.nih.gov/pubmed/26692124 http://dx.doi.org/10.1038/srep18708 |
work_keys_str_mv | AT kadonotakashi characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT miyagawayamaguchiarisa characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT kiranozomu characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT tomaruyuji characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT okamitakuma characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT yoshimatsutakamichi characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT houliyuan characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT ohamatakeshi characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT fukunagakazunari characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT okauchimasanori characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT yamaguchiharuo characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT ohnishikohei characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT falciatoreangela characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum AT adachimasao characterizationofmarinediatominfectingviruspromotersinthemodeldiatomphaeodactylumtricornutum |