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Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines
BACKGROUND: Natural products display numerous therapeutic properties (e.g., antibacterial activity), providing the population with countless benefits. Therefore, the search for novel biologically active, naturally occurring compounds is extremely important. The present paper describes the antibacter...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687089/ https://www.ncbi.nlm.nih.gov/pubmed/26691920 http://dx.doi.org/10.1186/s12906-015-0961-4 |
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author | Abrão, Fariza de Araújo Costa, Luciana Delfino Alves, Jacqueline Morais Senedese, Juliana Marques de Castro, Pâmela Tinti Ambrósio, Sérgio Ricardo Veneziani, Rodrigo Cássio Sola Bastos, Jairo Kenupp Tavares, Denise Crispim Martins, Carlos Henrique G. |
author_facet | Abrão, Fariza de Araújo Costa, Luciana Delfino Alves, Jacqueline Morais Senedese, Juliana Marques de Castro, Pâmela Tinti Ambrósio, Sérgio Ricardo Veneziani, Rodrigo Cássio Sola Bastos, Jairo Kenupp Tavares, Denise Crispim Martins, Carlos Henrique G. |
author_sort | Abrão, Fariza |
collection | PubMed |
description | BACKGROUND: Natural products display numerous therapeutic properties (e.g., antibacterial activity), providing the population with countless benefits. Therefore, the search for novel biologically active, naturally occurring compounds is extremely important. The present paper describes the antibacterial action of the Copaifera langsdorffii oleoresin and ten compounds isolated from this oleoresin against multiresistant bacteria; it also reports the antiproliferative activity of the Copaifera langsdorffii oleoresin and (-)-copalic acid. METHODS: MICs and MBCs were used to determine the antibacterial activity. Time-kill curve assays provided the time that was necessary for the bacteria to die. The Minimum Inhbitory Concentration of Biofilm (CIMB(50)) of the compounds that displayed the best results was calculated. Cytotoxicity was measured by using the XTT assay. RESULTS: The diterpene (-)-copalic acid was the most active antibacterial and afforded promising Minimum Inhibitory Concentration (MIC) values for most of the tested strains. Determination of the bactericidal kinetics against some bacteria revealed that the bactericidal effect emerged within six hours of incubation for Streptococcus pneumoniae. Concerning the antibiofilm action of this diterpene, its MICB(50) was twofold larger than its CBM against S. capitis and S. pneumoniae. The XTT assay helped to evaluate the cytotoxic effect; results are expressed as IC(50). The most pronounced antiproliferative effect arose in tumor cell lines treated with (-)-copalic acid; the lowest IC(50) value was found for the human glioblastoma cell line. CONCLUSIONS: The diterpene (-)-copalic acid is a potential lead for the development of new selective antimicrobial agents to treat infections caused by Gram-positive multiresistant microorganisms, in both the sessile and planktonic mode. This diterpene is also a good candidate to develop anticancer drugs. |
format | Online Article Text |
id | pubmed-4687089 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46870892015-12-23 Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines Abrão, Fariza de Araújo Costa, Luciana Delfino Alves, Jacqueline Morais Senedese, Juliana Marques de Castro, Pâmela Tinti Ambrósio, Sérgio Ricardo Veneziani, Rodrigo Cássio Sola Bastos, Jairo Kenupp Tavares, Denise Crispim Martins, Carlos Henrique G. BMC Complement Altern Med Research Article BACKGROUND: Natural products display numerous therapeutic properties (e.g., antibacterial activity), providing the population with countless benefits. Therefore, the search for novel biologically active, naturally occurring compounds is extremely important. The present paper describes the antibacterial action of the Copaifera langsdorffii oleoresin and ten compounds isolated from this oleoresin against multiresistant bacteria; it also reports the antiproliferative activity of the Copaifera langsdorffii oleoresin and (-)-copalic acid. METHODS: MICs and MBCs were used to determine the antibacterial activity. Time-kill curve assays provided the time that was necessary for the bacteria to die. The Minimum Inhbitory Concentration of Biofilm (CIMB(50)) of the compounds that displayed the best results was calculated. Cytotoxicity was measured by using the XTT assay. RESULTS: The diterpene (-)-copalic acid was the most active antibacterial and afforded promising Minimum Inhibitory Concentration (MIC) values for most of the tested strains. Determination of the bactericidal kinetics against some bacteria revealed that the bactericidal effect emerged within six hours of incubation for Streptococcus pneumoniae. Concerning the antibiofilm action of this diterpene, its MICB(50) was twofold larger than its CBM against S. capitis and S. pneumoniae. The XTT assay helped to evaluate the cytotoxic effect; results are expressed as IC(50). The most pronounced antiproliferative effect arose in tumor cell lines treated with (-)-copalic acid; the lowest IC(50) value was found for the human glioblastoma cell line. CONCLUSIONS: The diterpene (-)-copalic acid is a potential lead for the development of new selective antimicrobial agents to treat infections caused by Gram-positive multiresistant microorganisms, in both the sessile and planktonic mode. This diterpene is also a good candidate to develop anticancer drugs. BioMed Central 2015-12-21 /pmc/articles/PMC4687089/ /pubmed/26691920 http://dx.doi.org/10.1186/s12906-015-0961-4 Text en © Abrão et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Abrão, Fariza de Araújo Costa, Luciana Delfino Alves, Jacqueline Morais Senedese, Juliana Marques de Castro, Pâmela Tinti Ambrósio, Sérgio Ricardo Veneziani, Rodrigo Cássio Sola Bastos, Jairo Kenupp Tavares, Denise Crispim Martins, Carlos Henrique G. Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines |
title | Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines |
title_full | Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines |
title_fullStr | Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines |
title_full_unstemmed | Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines |
title_short | Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines |
title_sort | copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687089/ https://www.ncbi.nlm.nih.gov/pubmed/26691920 http://dx.doi.org/10.1186/s12906-015-0961-4 |
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