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Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines

BACKGROUND: Natural products display numerous therapeutic properties (e.g., antibacterial activity), providing the population with countless benefits. Therefore, the search for novel biologically active, naturally occurring compounds is extremely important. The present paper describes the antibacter...

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Autores principales: Abrão, Fariza, de Araújo Costa, Luciana Delfino, Alves, Jacqueline Morais, Senedese, Juliana Marques, de Castro, Pâmela Tinti, Ambrósio, Sérgio Ricardo, Veneziani, Rodrigo Cássio Sola, Bastos, Jairo Kenupp, Tavares, Denise Crispim, Martins, Carlos Henrique G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687089/
https://www.ncbi.nlm.nih.gov/pubmed/26691920
http://dx.doi.org/10.1186/s12906-015-0961-4
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author Abrão, Fariza
de Araújo Costa, Luciana Delfino
Alves, Jacqueline Morais
Senedese, Juliana Marques
de Castro, Pâmela Tinti
Ambrósio, Sérgio Ricardo
Veneziani, Rodrigo Cássio Sola
Bastos, Jairo Kenupp
Tavares, Denise Crispim
Martins, Carlos Henrique G.
author_facet Abrão, Fariza
de Araújo Costa, Luciana Delfino
Alves, Jacqueline Morais
Senedese, Juliana Marques
de Castro, Pâmela Tinti
Ambrósio, Sérgio Ricardo
Veneziani, Rodrigo Cássio Sola
Bastos, Jairo Kenupp
Tavares, Denise Crispim
Martins, Carlos Henrique G.
author_sort Abrão, Fariza
collection PubMed
description BACKGROUND: Natural products display numerous therapeutic properties (e.g., antibacterial activity), providing the population with countless benefits. Therefore, the search for novel biologically active, naturally occurring compounds is extremely important. The present paper describes the antibacterial action of the Copaifera langsdorffii oleoresin and ten compounds isolated from this oleoresin against multiresistant bacteria; it also reports the antiproliferative activity of the Copaifera langsdorffii oleoresin and (-)-copalic acid. METHODS: MICs and MBCs were used to determine the antibacterial activity. Time-kill curve assays provided the time that was necessary for the bacteria to die. The Minimum Inhbitory Concentration of Biofilm (CIMB(50)) of the compounds that displayed the best results was calculated. Cytotoxicity was measured by using the XTT assay. RESULTS: The diterpene (-)-copalic acid was the most active antibacterial and afforded promising Minimum Inhibitory Concentration (MIC) values for most of the tested strains. Determination of the bactericidal kinetics against some bacteria revealed that the bactericidal effect emerged within six hours of incubation for Streptococcus pneumoniae. Concerning the antibiofilm action of this diterpene, its MICB(50) was twofold larger than its CBM against S. capitis and S. pneumoniae. The XTT assay helped to evaluate the cytotoxic effect; results are expressed as IC(50). The most pronounced antiproliferative effect arose in tumor cell lines treated with (-)-copalic acid; the lowest IC(50) value was found for the human glioblastoma cell line. CONCLUSIONS: The diterpene (-)-copalic acid is a potential lead for the development of new selective antimicrobial agents to treat infections caused by Gram-positive multiresistant microorganisms, in both the sessile and planktonic mode. This diterpene is also a good candidate to develop anticancer drugs.
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spelling pubmed-46870892015-12-23 Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines Abrão, Fariza de Araújo Costa, Luciana Delfino Alves, Jacqueline Morais Senedese, Juliana Marques de Castro, Pâmela Tinti Ambrósio, Sérgio Ricardo Veneziani, Rodrigo Cássio Sola Bastos, Jairo Kenupp Tavares, Denise Crispim Martins, Carlos Henrique G. BMC Complement Altern Med Research Article BACKGROUND: Natural products display numerous therapeutic properties (e.g., antibacterial activity), providing the population with countless benefits. Therefore, the search for novel biologically active, naturally occurring compounds is extremely important. The present paper describes the antibacterial action of the Copaifera langsdorffii oleoresin and ten compounds isolated from this oleoresin against multiresistant bacteria; it also reports the antiproliferative activity of the Copaifera langsdorffii oleoresin and (-)-copalic acid. METHODS: MICs and MBCs were used to determine the antibacterial activity. Time-kill curve assays provided the time that was necessary for the bacteria to die. The Minimum Inhbitory Concentration of Biofilm (CIMB(50)) of the compounds that displayed the best results was calculated. Cytotoxicity was measured by using the XTT assay. RESULTS: The diterpene (-)-copalic acid was the most active antibacterial and afforded promising Minimum Inhibitory Concentration (MIC) values for most of the tested strains. Determination of the bactericidal kinetics against some bacteria revealed that the bactericidal effect emerged within six hours of incubation for Streptococcus pneumoniae. Concerning the antibiofilm action of this diterpene, its MICB(50) was twofold larger than its CBM against S. capitis and S. pneumoniae. The XTT assay helped to evaluate the cytotoxic effect; results are expressed as IC(50). The most pronounced antiproliferative effect arose in tumor cell lines treated with (-)-copalic acid; the lowest IC(50) value was found for the human glioblastoma cell line. CONCLUSIONS: The diterpene (-)-copalic acid is a potential lead for the development of new selective antimicrobial agents to treat infections caused by Gram-positive multiresistant microorganisms, in both the sessile and planktonic mode. This diterpene is also a good candidate to develop anticancer drugs. BioMed Central 2015-12-21 /pmc/articles/PMC4687089/ /pubmed/26691920 http://dx.doi.org/10.1186/s12906-015-0961-4 Text en © Abrão et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Abrão, Fariza
de Araújo Costa, Luciana Delfino
Alves, Jacqueline Morais
Senedese, Juliana Marques
de Castro, Pâmela Tinti
Ambrósio, Sérgio Ricardo
Veneziani, Rodrigo Cássio Sola
Bastos, Jairo Kenupp
Tavares, Denise Crispim
Martins, Carlos Henrique G.
Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines
title Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines
title_full Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines
title_fullStr Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines
title_full_unstemmed Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines
title_short Copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines
title_sort copaifera langsdorffii oleoresin and its isolated compounds: antibacterial effect and antiproliferative activity in cancer cell lines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687089/
https://www.ncbi.nlm.nih.gov/pubmed/26691920
http://dx.doi.org/10.1186/s12906-015-0961-4
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