Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois

BACKGROUND: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are available on the presence of Giardia duodenalis infecting these species. METHODS: A total of 157 fecal samples from Alpine Rupicapra rupicaprarupicapra and Apennine Rupicapra pyrenaicaornata c...

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Autores principales: De Liberato, Claudio, Berrilli, Federica, Marangi, Marianna, Santoro, Maristella, Trogu, Tiziana, Putignani, Lorenza, Lanfranchi, Paolo, Ferretti, Francesco, D’Amelio, Stefano, Giangaspero, Annunziata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687356/
https://www.ncbi.nlm.nih.gov/pubmed/26691178
http://dx.doi.org/10.1186/s13071-015-1243-1
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author De Liberato, Claudio
Berrilli, Federica
Marangi, Marianna
Santoro, Maristella
Trogu, Tiziana
Putignani, Lorenza
Lanfranchi, Paolo
Ferretti, Francesco
D’Amelio, Stefano
Giangaspero, Annunziata
author_facet De Liberato, Claudio
Berrilli, Federica
Marangi, Marianna
Santoro, Maristella
Trogu, Tiziana
Putignani, Lorenza
Lanfranchi, Paolo
Ferretti, Francesco
D’Amelio, Stefano
Giangaspero, Annunziata
author_sort De Liberato, Claudio
collection PubMed
description BACKGROUND: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are available on the presence of Giardia duodenalis infecting these species. METHODS: A total of 157 fecal samples from Alpine Rupicapra rupicaprarupicapra and Apennine Rupicapra pyrenaicaornata chamois were tested for the presence of G. duodenalis by immunofluorescence test, quantitative Real Time PCR and end-point PCR for genotype characterization. RESULTS: G. duodenalis was detected in R. r. rupicapra and R. p. ornata, with a percentage value of 4.45 (5.82 and 1.85 %, respectively), and a cyst burden of up to 31,800 cysts/g of feces. Assemblages A/AI and E were identified in R. r. rupicapra and assemblage A/AIII in R. p. ornata. CONCLUSIONS: The present study represents the first record of Giardia duodenalis in Rupicapra spp., suggesting that these wild bovids can play an epidemiological role in environmental contamination and transmission of both zoonotic and non-zoonotic genotypes.
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spelling pubmed-46873562015-12-23 Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois De Liberato, Claudio Berrilli, Federica Marangi, Marianna Santoro, Maristella Trogu, Tiziana Putignani, Lorenza Lanfranchi, Paolo Ferretti, Francesco D’Amelio, Stefano Giangaspero, Annunziata Parasit Vectors Research BACKGROUND: Although chamois Rupicapra spp. are the most abundant mountain ungulates in Europe, no data are available on the presence of Giardia duodenalis infecting these species. METHODS: A total of 157 fecal samples from Alpine Rupicapra rupicaprarupicapra and Apennine Rupicapra pyrenaicaornata chamois were tested for the presence of G. duodenalis by immunofluorescence test, quantitative Real Time PCR and end-point PCR for genotype characterization. RESULTS: G. duodenalis was detected in R. r. rupicapra and R. p. ornata, with a percentage value of 4.45 (5.82 and 1.85 %, respectively), and a cyst burden of up to 31,800 cysts/g of feces. Assemblages A/AI and E were identified in R. r. rupicapra and assemblage A/AIII in R. p. ornata. CONCLUSIONS: The present study represents the first record of Giardia duodenalis in Rupicapra spp., suggesting that these wild bovids can play an epidemiological role in environmental contamination and transmission of both zoonotic and non-zoonotic genotypes. BioMed Central 2015-12-21 /pmc/articles/PMC4687356/ /pubmed/26691178 http://dx.doi.org/10.1186/s13071-015-1243-1 Text en © De Liberato et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
De Liberato, Claudio
Berrilli, Federica
Marangi, Marianna
Santoro, Maristella
Trogu, Tiziana
Putignani, Lorenza
Lanfranchi, Paolo
Ferretti, Francesco
D’Amelio, Stefano
Giangaspero, Annunziata
Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois
title Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois
title_full Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois
title_fullStr Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois
title_full_unstemmed Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois
title_short Giardia duodenalis in Alpine (Rupicapra rupicapra rupicapra) and Apennine (Rupicapra pyrenaica ornata) chamois
title_sort giardia duodenalis in alpine (rupicapra rupicapra rupicapra) and apennine (rupicapra pyrenaica ornata) chamois
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687356/
https://www.ncbi.nlm.nih.gov/pubmed/26691178
http://dx.doi.org/10.1186/s13071-015-1243-1
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