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Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012

BACKGROUND: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. OBJECTIVE: To character...

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Autores principales: Perglione, Cecilia Olguin, Gildea, Sarah, Rimondi, Agustina, Miño, Samuel, Vissani, Aldana, Carossino, Mariano, Cullinane, Ann, Barrandeguy, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687505/
https://www.ncbi.nlm.nih.gov/pubmed/26406274
http://dx.doi.org/10.1111/irv.12349
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author Perglione, Cecilia Olguin
Gildea, Sarah
Rimondi, Agustina
Miño, Samuel
Vissani, Aldana
Carossino, Mariano
Cullinane, Ann
Barrandeguy, Maria
author_facet Perglione, Cecilia Olguin
Gildea, Sarah
Rimondi, Agustina
Miño, Samuel
Vissani, Aldana
Carossino, Mariano
Cullinane, Ann
Barrandeguy, Maria
author_sort Perglione, Cecilia Olguin
collection PubMed
description BACKGROUND: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. OBJECTIVE: To characterise the EI viruses detected during the outbreak in Uruguay and Argentina. METHODS: Virus was detected in nasopharyngeal swabs by a pan‐reactive influenza type A real‐time RT‐PCR. The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. Paired serum samples were tested by haemagglutination inhibition and single radial haemolysis. RESULTS: The diagnosis of EIV was confirmed by real‐time RT‐PCR, virus isolation and serological testing. The phylogenetic analysis of HA1 gene sequences of 18 EI viruses indicated that all of them belong to clade 1 of the Florida sublineage of the American lineage and are closely related to viruses isolated in the United States in 2012. The HA1 of viruses identified in horses in racing facilities in Maroñas, Uruguay, and in Palermo, Argentina, displayed 100% amino acid sequence identity and were identical to that of a virus isolated in Dubai in 2012, from vaccinated endurance horses recently imported from Uruguay. CONCLUSIONS: The surveillance data reported illustrate the international spread of EI viruses and support the recommendations of the OIE expert surveillance panel to include viruses of the Florida sublineage in vaccines.
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spelling pubmed-46875052016-01-01 Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012 Perglione, Cecilia Olguin Gildea, Sarah Rimondi, Agustina Miño, Samuel Vissani, Aldana Carossino, Mariano Cullinane, Ann Barrandeguy, Maria Influenza Other Respir Viruses Original Articles BACKGROUND: In 2012, equine influenza (EI) virus was confirmed as the cause of outbreaks of respiratory disease in horses throughout South America. In Uruguay and Argentina, hundreds of vaccinated thoroughbred horses in training and racing facilities were clinically affected. OBJECTIVE: To characterise the EI viruses detected during the outbreak in Uruguay and Argentina. METHODS: Virus was detected in nasopharyngeal swabs by a pan‐reactive influenza type A real‐time RT‐PCR. The nucleotide sequence of the HA1 gene was determined and analysed phylogenetically using mega 5 software. Amino acid sequences alignments were constructed and virus was antigenically characterised with specific ferret antisera. Paired serum samples were tested by haemagglutination inhibition and single radial haemolysis. RESULTS: The diagnosis of EIV was confirmed by real‐time RT‐PCR, virus isolation and serological testing. The phylogenetic analysis of HA1 gene sequences of 18 EI viruses indicated that all of them belong to clade 1 of the Florida sublineage of the American lineage and are closely related to viruses isolated in the United States in 2012. The HA1 of viruses identified in horses in racing facilities in Maroñas, Uruguay, and in Palermo, Argentina, displayed 100% amino acid sequence identity and were identical to that of a virus isolated in Dubai in 2012, from vaccinated endurance horses recently imported from Uruguay. CONCLUSIONS: The surveillance data reported illustrate the international spread of EI viruses and support the recommendations of the OIE expert surveillance panel to include viruses of the Florida sublineage in vaccines. John Wiley and Sons Inc. 2015-12-11 2016-01 /pmc/articles/PMC4687505/ /pubmed/26406274 http://dx.doi.org/10.1111/irv.12349 Text en © 2015 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Perglione, Cecilia Olguin
Gildea, Sarah
Rimondi, Agustina
Miño, Samuel
Vissani, Aldana
Carossino, Mariano
Cullinane, Ann
Barrandeguy, Maria
Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title_full Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title_fullStr Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title_full_unstemmed Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title_short Epidemiological and virological findings during multiple outbreaks of equine influenza in South America in 2012
title_sort epidemiological and virological findings during multiple outbreaks of equine influenza in south america in 2012
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687505/
https://www.ncbi.nlm.nih.gov/pubmed/26406274
http://dx.doi.org/10.1111/irv.12349
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