Cargando…

Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin

Mast cell (MC) activation contributes considerably to immune responses, such as host protection and allergy. Cell surface immunoreceptors expressed on MCs play an important role in MC activation. Although various immunoreceptors on MCs have been identified, the regulatory mechanism of MC activation...

Descripción completa

Detalles Bibliográficos
Autores principales: Kanemaru, Kazumasa, Noguchi, Emiko, Tokunaga, Takahiro, Nagai, Kei, Hiroyama, Takashi, Nakamura, Yukio, Tahara-Hanaoka, Satoko, Shibuya, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687632/
https://www.ncbi.nlm.nih.gov/pubmed/26659448
http://dx.doi.org/10.1371/journal.pone.0144436
_version_ 1782406650309640192
author Kanemaru, Kazumasa
Noguchi, Emiko
Tokunaga, Takahiro
Nagai, Kei
Hiroyama, Takashi
Nakamura, Yukio
Tahara-Hanaoka, Satoko
Shibuya, Akira
author_facet Kanemaru, Kazumasa
Noguchi, Emiko
Tokunaga, Takahiro
Nagai, Kei
Hiroyama, Takashi
Nakamura, Yukio
Tahara-Hanaoka, Satoko
Shibuya, Akira
author_sort Kanemaru, Kazumasa
collection PubMed
description Mast cell (MC) activation contributes considerably to immune responses, such as host protection and allergy. Cell surface immunoreceptors expressed on MCs play an important role in MC activation. Although various immunoreceptors on MCs have been identified, the regulatory mechanism of MC activation is not fully understood. To understand the regulatory mechanisms of MC activation, we used gene expression analyses of human and mouse MCs to identify a novel immunoreceptor expressed on MCs. We found that Tek, which encodes Tie2, was preferentially expressed in the MCs of both humans and mice. However, Tie2 was not detected on the cell surface of the mouse MCs of the peritoneal cavity, ear skin, or colon lamina propria. In contrast, it was expressed on mouse bone marrow–derived MCs and bone marrow MC progenitors (BM-MCps). Stimulation of Tie2 by its ligand angiopoietin-1 induced tyrosine phosphorylation of Tie2 in MEDMC-BRC6, a mouse embryonic stem cell-derived mast cell line, and enhanced MEDMC-BRC6 and mouse BM-MCp adhesion to vascular cell adhesion molecule-1 (VCAM-1) through α4β1 integrin. These results suggest that Tie2 signaling induces α4β1 integrin activation on BM-MCps for adhesion to VCAM-1.
format Online
Article
Text
id pubmed-4687632
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-46876322015-12-31 Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin Kanemaru, Kazumasa Noguchi, Emiko Tokunaga, Takahiro Nagai, Kei Hiroyama, Takashi Nakamura, Yukio Tahara-Hanaoka, Satoko Shibuya, Akira PLoS One Research Article Mast cell (MC) activation contributes considerably to immune responses, such as host protection and allergy. Cell surface immunoreceptors expressed on MCs play an important role in MC activation. Although various immunoreceptors on MCs have been identified, the regulatory mechanism of MC activation is not fully understood. To understand the regulatory mechanisms of MC activation, we used gene expression analyses of human and mouse MCs to identify a novel immunoreceptor expressed on MCs. We found that Tek, which encodes Tie2, was preferentially expressed in the MCs of both humans and mice. However, Tie2 was not detected on the cell surface of the mouse MCs of the peritoneal cavity, ear skin, or colon lamina propria. In contrast, it was expressed on mouse bone marrow–derived MCs and bone marrow MC progenitors (BM-MCps). Stimulation of Tie2 by its ligand angiopoietin-1 induced tyrosine phosphorylation of Tie2 in MEDMC-BRC6, a mouse embryonic stem cell-derived mast cell line, and enhanced MEDMC-BRC6 and mouse BM-MCp adhesion to vascular cell adhesion molecule-1 (VCAM-1) through α4β1 integrin. These results suggest that Tie2 signaling induces α4β1 integrin activation on BM-MCps for adhesion to VCAM-1. Public Library of Science 2015-12-11 /pmc/articles/PMC4687632/ /pubmed/26659448 http://dx.doi.org/10.1371/journal.pone.0144436 Text en © 2015 Kanemaru et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kanemaru, Kazumasa
Noguchi, Emiko
Tokunaga, Takahiro
Nagai, Kei
Hiroyama, Takashi
Nakamura, Yukio
Tahara-Hanaoka, Satoko
Shibuya, Akira
Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin
title Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin
title_full Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin
title_fullStr Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin
title_full_unstemmed Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin
title_short Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin
title_sort tie2 signaling enhances mast cell progenitor adhesion to vascular cell adhesion molecule-1 (vcam-1) through α4β1 integrin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687632/
https://www.ncbi.nlm.nih.gov/pubmed/26659448
http://dx.doi.org/10.1371/journal.pone.0144436
work_keys_str_mv AT kanemarukazumasa tie2signalingenhancesmastcellprogenitoradhesiontovascularcelladhesionmolecule1vcam1througha4b1integrin
AT noguchiemiko tie2signalingenhancesmastcellprogenitoradhesiontovascularcelladhesionmolecule1vcam1througha4b1integrin
AT tokunagatakahiro tie2signalingenhancesmastcellprogenitoradhesiontovascularcelladhesionmolecule1vcam1througha4b1integrin
AT nagaikei tie2signalingenhancesmastcellprogenitoradhesiontovascularcelladhesionmolecule1vcam1througha4b1integrin
AT hiroyamatakashi tie2signalingenhancesmastcellprogenitoradhesiontovascularcelladhesionmolecule1vcam1througha4b1integrin
AT nakamurayukio tie2signalingenhancesmastcellprogenitoradhesiontovascularcelladhesionmolecule1vcam1througha4b1integrin
AT taharahanaokasatoko tie2signalingenhancesmastcellprogenitoradhesiontovascularcelladhesionmolecule1vcam1througha4b1integrin
AT shibuyaakira tie2signalingenhancesmastcellprogenitoradhesiontovascularcelladhesionmolecule1vcam1througha4b1integrin