Cargando…
Sensing Size through Clustering in Non-Equilibrium Membranes and the Control of Membrane-Bound Enzymatic Reactions
The formation of dynamical clusters of proteins is ubiquitous in cellular membranes and is in part regulated by the recycling of membrane components. We show, using stochastic simulations and analytic modeling, that the out-of-equilibrium cluster size distribution of membrane components undergoing c...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687633/ https://www.ncbi.nlm.nih.gov/pubmed/26656912 http://dx.doi.org/10.1371/journal.pone.0143470 |
_version_ | 1782406650535084032 |
---|---|
author | Vagne, Quentin Turner, Matthew S. Sens, Pierre |
author_facet | Vagne, Quentin Turner, Matthew S. Sens, Pierre |
author_sort | Vagne, Quentin |
collection | PubMed |
description | The formation of dynamical clusters of proteins is ubiquitous in cellular membranes and is in part regulated by the recycling of membrane components. We show, using stochastic simulations and analytic modeling, that the out-of-equilibrium cluster size distribution of membrane components undergoing continuous recycling is strongly influenced by lateral confinement. This result has significant implications for the clustering of plasma membrane proteins whose mobility is hindered by cytoskeletal “corrals” and for protein clustering in cellular organelles of limited size that generically support material fluxes. We show how the confinement size can be sensed through its effect on the size distribution of clusters of membrane heterogeneities and propose that this could be regulated to control the efficiency of membrane-bound reactions. To illustrate this, we study a chain of enzymatic reactions sensitive to membrane protein clustering. The reaction efficiency is found to be a non-monotonic function of the system size, and can be optimal for sizes comparable to those of cellular organelles. |
format | Online Article Text |
id | pubmed-4687633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46876332015-12-31 Sensing Size through Clustering in Non-Equilibrium Membranes and the Control of Membrane-Bound Enzymatic Reactions Vagne, Quentin Turner, Matthew S. Sens, Pierre PLoS One Research Article The formation of dynamical clusters of proteins is ubiquitous in cellular membranes and is in part regulated by the recycling of membrane components. We show, using stochastic simulations and analytic modeling, that the out-of-equilibrium cluster size distribution of membrane components undergoing continuous recycling is strongly influenced by lateral confinement. This result has significant implications for the clustering of plasma membrane proteins whose mobility is hindered by cytoskeletal “corrals” and for protein clustering in cellular organelles of limited size that generically support material fluxes. We show how the confinement size can be sensed through its effect on the size distribution of clusters of membrane heterogeneities and propose that this could be regulated to control the efficiency of membrane-bound reactions. To illustrate this, we study a chain of enzymatic reactions sensitive to membrane protein clustering. The reaction efficiency is found to be a non-monotonic function of the system size, and can be optimal for sizes comparable to those of cellular organelles. Public Library of Science 2015-12-14 /pmc/articles/PMC4687633/ /pubmed/26656912 http://dx.doi.org/10.1371/journal.pone.0143470 Text en © 2015 Vagne et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Vagne, Quentin Turner, Matthew S. Sens, Pierre Sensing Size through Clustering in Non-Equilibrium Membranes and the Control of Membrane-Bound Enzymatic Reactions |
title | Sensing Size through Clustering in Non-Equilibrium Membranes and the Control of Membrane-Bound Enzymatic Reactions |
title_full | Sensing Size through Clustering in Non-Equilibrium Membranes and the Control of Membrane-Bound Enzymatic Reactions |
title_fullStr | Sensing Size through Clustering in Non-Equilibrium Membranes and the Control of Membrane-Bound Enzymatic Reactions |
title_full_unstemmed | Sensing Size through Clustering in Non-Equilibrium Membranes and the Control of Membrane-Bound Enzymatic Reactions |
title_short | Sensing Size through Clustering in Non-Equilibrium Membranes and the Control of Membrane-Bound Enzymatic Reactions |
title_sort | sensing size through clustering in non-equilibrium membranes and the control of membrane-bound enzymatic reactions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687633/ https://www.ncbi.nlm.nih.gov/pubmed/26656912 http://dx.doi.org/10.1371/journal.pone.0143470 |
work_keys_str_mv | AT vagnequentin sensingsizethroughclusteringinnonequilibriummembranesandthecontrolofmembraneboundenzymaticreactions AT turnermatthews sensingsizethroughclusteringinnonequilibriummembranesandthecontrolofmembraneboundenzymaticreactions AT senspierre sensingsizethroughclusteringinnonequilibriummembranesandthecontrolofmembraneboundenzymaticreactions |