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Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis

In recent years, gold nanoparticles (AuNPs) have become the focus of much attention in biomedical research, especially in the context of nanomedicine, due to their distinctive physicochemical properties. The current study was planned to assess the effect of three dose levels of AuNPs on the gene exp...

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Autores principales: Dkhil, Mohamed A, Bauomy, Amira A, Diab, Marwa SM, Al-Quraishy, Saleh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687720/
https://www.ncbi.nlm.nih.gov/pubmed/26719689
http://dx.doi.org/10.2147/IJN.S97622
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author Dkhil, Mohamed A
Bauomy, Amira A
Diab, Marwa SM
Al-Quraishy, Saleh
author_facet Dkhil, Mohamed A
Bauomy, Amira A
Diab, Marwa SM
Al-Quraishy, Saleh
author_sort Dkhil, Mohamed A
collection PubMed
description In recent years, gold nanoparticles (AuNPs) have become the focus of much attention in biomedical research, especially in the context of nanomedicine, due to their distinctive physicochemical properties. The current study was planned to assess the effect of three dose levels of AuNPs on the gene expression, histology, and oxidative stress status of Schistosoma mansoni-infected mice liver. Inoculation of mice with 100 μL AuNPs at different doses (0.25, 0.5, and 1 mg/kg mice body weight) twice on day 46 and day 49 postinfection reduced the total worm burden, the egg load in the liver, and the granuloma size. AuNPs also appeared to decrease the activities of malondialdehyde and nitric oxide significantly, and increase the level of glutathione compared to the infected untreated group. Concomitantly, AuNPs ameliorated the inflammatory response by decreasing the mRNA expression of interleukin-1β, interleukin-6, tumor necrosis factor-α, interferon-γ, and inducible nitric oxide synthase. These consistent molecular, histopathological, and biochemical data suggest that AuNPs could ameliorate infection-induced damage in the livers of mice. Our results indicated that AuNPs are effective anti-schistosomal and antioxidant agents. Further confirmation of the role of nanogold as an anti-schistosomal agent, as well as its mechanism of action, requires further studies to be undertaken in the future.
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spelling pubmed-46877202015-12-30 Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis Dkhil, Mohamed A Bauomy, Amira A Diab, Marwa SM Al-Quraishy, Saleh Int J Nanomedicine Original Research In recent years, gold nanoparticles (AuNPs) have become the focus of much attention in biomedical research, especially in the context of nanomedicine, due to their distinctive physicochemical properties. The current study was planned to assess the effect of three dose levels of AuNPs on the gene expression, histology, and oxidative stress status of Schistosoma mansoni-infected mice liver. Inoculation of mice with 100 μL AuNPs at different doses (0.25, 0.5, and 1 mg/kg mice body weight) twice on day 46 and day 49 postinfection reduced the total worm burden, the egg load in the liver, and the granuloma size. AuNPs also appeared to decrease the activities of malondialdehyde and nitric oxide significantly, and increase the level of glutathione compared to the infected untreated group. Concomitantly, AuNPs ameliorated the inflammatory response by decreasing the mRNA expression of interleukin-1β, interleukin-6, tumor necrosis factor-α, interferon-γ, and inducible nitric oxide synthase. These consistent molecular, histopathological, and biochemical data suggest that AuNPs could ameliorate infection-induced damage in the livers of mice. Our results indicated that AuNPs are effective anti-schistosomal and antioxidant agents. Further confirmation of the role of nanogold as an anti-schistosomal agent, as well as its mechanism of action, requires further studies to be undertaken in the future. Dove Medical Press 2015-12-16 /pmc/articles/PMC4687720/ /pubmed/26719689 http://dx.doi.org/10.2147/IJN.S97622 Text en © 2015 Dkhil et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Dkhil, Mohamed A
Bauomy, Amira A
Diab, Marwa SM
Al-Quraishy, Saleh
Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_full Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_fullStr Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_full_unstemmed Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_short Antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
title_sort antioxidant and hepatoprotective role of gold nanoparticles against murine hepatic schistosomiasis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687720/
https://www.ncbi.nlm.nih.gov/pubmed/26719689
http://dx.doi.org/10.2147/IJN.S97622
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