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The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes

The critical step in meiosis is to attach homologous chromosomes to the opposite poles. In mouse oocytes, stable microtubule end-on attachments to kinetochores are not established until hours after spindle assembly, and phosphorylation of kinetochore proteins by Aurora B/C is responsible for the del...

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Autores principales: Głuszek, A. Agata, Cullen, C. Fiona, Li, Wenjing, Battaglia, Rachel A., Radford, Sarah J., Costa, Mariana F., McKim, Kim S., Goshima, Gohta, Ohkura, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687879/
https://www.ncbi.nlm.nih.gov/pubmed/26668329
http://dx.doi.org/10.1083/jcb.201507006
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author Głuszek, A. Agata
Cullen, C. Fiona
Li, Wenjing
Battaglia, Rachel A.
Radford, Sarah J.
Costa, Mariana F.
McKim, Kim S.
Goshima, Gohta
Ohkura, Hiroyuki
author_facet Głuszek, A. Agata
Cullen, C. Fiona
Li, Wenjing
Battaglia, Rachel A.
Radford, Sarah J.
Costa, Mariana F.
McKim, Kim S.
Goshima, Gohta
Ohkura, Hiroyuki
author_sort Głuszek, A. Agata
collection PubMed
description The critical step in meiosis is to attach homologous chromosomes to the opposite poles. In mouse oocytes, stable microtubule end-on attachments to kinetochores are not established until hours after spindle assembly, and phosphorylation of kinetochore proteins by Aurora B/C is responsible for the delay. Here we demonstrated that microtubule ends are actively prevented from stable attachment to kinetochores until well after spindle formation in Drosophila melanogaster oocytes. We identified the microtubule catastrophe-promoting complex Sentin-EB1 as a major factor responsible for this delay. Without this activity, microtubule ends precociously form robust attachments to kinetochores in oocytes, leading to a high proportion of homologous kinetochores stably attached to the same pole. Therefore, regulation of microtubule ends provides an alternative novel mechanism to delay stable kinetochore–microtubule attachment in oocytes.
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spelling pubmed-46878792016-06-21 The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes Głuszek, A. Agata Cullen, C. Fiona Li, Wenjing Battaglia, Rachel A. Radford, Sarah J. Costa, Mariana F. McKim, Kim S. Goshima, Gohta Ohkura, Hiroyuki J Cell Biol Research Articles The critical step in meiosis is to attach homologous chromosomes to the opposite poles. In mouse oocytes, stable microtubule end-on attachments to kinetochores are not established until hours after spindle assembly, and phosphorylation of kinetochore proteins by Aurora B/C is responsible for the delay. Here we demonstrated that microtubule ends are actively prevented from stable attachment to kinetochores until well after spindle formation in Drosophila melanogaster oocytes. We identified the microtubule catastrophe-promoting complex Sentin-EB1 as a major factor responsible for this delay. Without this activity, microtubule ends precociously form robust attachments to kinetochores in oocytes, leading to a high proportion of homologous kinetochores stably attached to the same pole. Therefore, regulation of microtubule ends provides an alternative novel mechanism to delay stable kinetochore–microtubule attachment in oocytes. The Rockefeller University Press 2015-12-21 /pmc/articles/PMC4687879/ /pubmed/26668329 http://dx.doi.org/10.1083/jcb.201507006 Text en © 2015 Gluszek et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Głuszek, A. Agata
Cullen, C. Fiona
Li, Wenjing
Battaglia, Rachel A.
Radford, Sarah J.
Costa, Mariana F.
McKim, Kim S.
Goshima, Gohta
Ohkura, Hiroyuki
The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes
title The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes
title_full The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes
title_fullStr The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes
title_full_unstemmed The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes
title_short The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes
title_sort microtubule catastrophe promoter sentin delays stable kinetochore–microtubule attachment in oocytes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687879/
https://www.ncbi.nlm.nih.gov/pubmed/26668329
http://dx.doi.org/10.1083/jcb.201507006
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