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The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes
The critical step in meiosis is to attach homologous chromosomes to the opposite poles. In mouse oocytes, stable microtubule end-on attachments to kinetochores are not established until hours after spindle assembly, and phosphorylation of kinetochore proteins by Aurora B/C is responsible for the del...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687879/ https://www.ncbi.nlm.nih.gov/pubmed/26668329 http://dx.doi.org/10.1083/jcb.201507006 |
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author | Głuszek, A. Agata Cullen, C. Fiona Li, Wenjing Battaglia, Rachel A. Radford, Sarah J. Costa, Mariana F. McKim, Kim S. Goshima, Gohta Ohkura, Hiroyuki |
author_facet | Głuszek, A. Agata Cullen, C. Fiona Li, Wenjing Battaglia, Rachel A. Radford, Sarah J. Costa, Mariana F. McKim, Kim S. Goshima, Gohta Ohkura, Hiroyuki |
author_sort | Głuszek, A. Agata |
collection | PubMed |
description | The critical step in meiosis is to attach homologous chromosomes to the opposite poles. In mouse oocytes, stable microtubule end-on attachments to kinetochores are not established until hours after spindle assembly, and phosphorylation of kinetochore proteins by Aurora B/C is responsible for the delay. Here we demonstrated that microtubule ends are actively prevented from stable attachment to kinetochores until well after spindle formation in Drosophila melanogaster oocytes. We identified the microtubule catastrophe-promoting complex Sentin-EB1 as a major factor responsible for this delay. Without this activity, microtubule ends precociously form robust attachments to kinetochores in oocytes, leading to a high proportion of homologous kinetochores stably attached to the same pole. Therefore, regulation of microtubule ends provides an alternative novel mechanism to delay stable kinetochore–microtubule attachment in oocytes. |
format | Online Article Text |
id | pubmed-4687879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46878792016-06-21 The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes Głuszek, A. Agata Cullen, C. Fiona Li, Wenjing Battaglia, Rachel A. Radford, Sarah J. Costa, Mariana F. McKim, Kim S. Goshima, Gohta Ohkura, Hiroyuki J Cell Biol Research Articles The critical step in meiosis is to attach homologous chromosomes to the opposite poles. In mouse oocytes, stable microtubule end-on attachments to kinetochores are not established until hours after spindle assembly, and phosphorylation of kinetochore proteins by Aurora B/C is responsible for the delay. Here we demonstrated that microtubule ends are actively prevented from stable attachment to kinetochores until well after spindle formation in Drosophila melanogaster oocytes. We identified the microtubule catastrophe-promoting complex Sentin-EB1 as a major factor responsible for this delay. Without this activity, microtubule ends precociously form robust attachments to kinetochores in oocytes, leading to a high proportion of homologous kinetochores stably attached to the same pole. Therefore, regulation of microtubule ends provides an alternative novel mechanism to delay stable kinetochore–microtubule attachment in oocytes. The Rockefeller University Press 2015-12-21 /pmc/articles/PMC4687879/ /pubmed/26668329 http://dx.doi.org/10.1083/jcb.201507006 Text en © 2015 Gluszek et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Głuszek, A. Agata Cullen, C. Fiona Li, Wenjing Battaglia, Rachel A. Radford, Sarah J. Costa, Mariana F. McKim, Kim S. Goshima, Gohta Ohkura, Hiroyuki The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes |
title | The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes |
title_full | The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes |
title_fullStr | The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes |
title_full_unstemmed | The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes |
title_short | The microtubule catastrophe promoter Sentin delays stable kinetochore–microtubule attachment in oocytes |
title_sort | microtubule catastrophe promoter sentin delays stable kinetochore–microtubule attachment in oocytes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687879/ https://www.ncbi.nlm.nih.gov/pubmed/26668329 http://dx.doi.org/10.1083/jcb.201507006 |
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