Cargando…
Protection against H5N1 Influenza Virus Induced by Matrix-M Adjuvanted Seasonal Virosomal Vaccine in Mice Requires Both Antibodies and T Cells
BACKGROUND: It remains important to develop the next generation of influenza vaccines that can provide protection against vaccine mismatched strains and to be prepared for potential pandemic outbreaks. To achieve this, the understanding of the immunological parameters that mediate such broad protect...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687931/ https://www.ncbi.nlm.nih.gov/pubmed/26696245 http://dx.doi.org/10.1371/journal.pone.0145243 |
_version_ | 1782406691052060672 |
---|---|
author | Cox, Freek Baart, Matthijs Huizingh, Jeroen Tolboom, Jeroen Dekking, Liesbeth Goudsmit, Jaap Saeland, Eirikur Radošević, Katarina |
author_facet | Cox, Freek Baart, Matthijs Huizingh, Jeroen Tolboom, Jeroen Dekking, Liesbeth Goudsmit, Jaap Saeland, Eirikur Radošević, Katarina |
author_sort | Cox, Freek |
collection | PubMed |
description | BACKGROUND: It remains important to develop the next generation of influenza vaccines that can provide protection against vaccine mismatched strains and to be prepared for potential pandemic outbreaks. To achieve this, the understanding of the immunological parameters that mediate such broad protection is crucial. METHOD: In the current study we assessed the contribution of humoral and cellular immune responses to heterosubtypic protection against H5N1 induced by a Matrix-M (MM) adjuvanted seasonal influenza vaccine by serum transfer and T-cell depletion studies. RESULTS: We demonstrate that the heterosubtypic protection against H5N1 induced by MM adjuvanted vaccine is partially mediated by antibodies. The serum contained both H5N1 cross-reactive hemagglutinin (HA)- and neuraminidase (NA)-specific antibodies but with limited virus neutralizing and no hemagglutination inhibiting activity. The cross-reactive antibodies induced antibody-dependent cellular cytotoxicity (ADCC) in vitro, suggesting a role for the Fc part of the antibodies in protection against H5N1. Besides H5N1 specific antibody responses, cross-reactive HA- and NA-specific T-cell responses were induced by the adjuvanted vaccine. T-cell depletion experiments demonstrated that both CD4(+) and CD8(+) T cells contribute to protection. CONCLUSION: Our study demonstrates that cross-protection against H5N1 induced by MM adjuvanted seasonal virosomal influenza vaccine requires both the humoral and cellular arm of the immune system. |
format | Online Article Text |
id | pubmed-4687931 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46879312015-12-31 Protection against H5N1 Influenza Virus Induced by Matrix-M Adjuvanted Seasonal Virosomal Vaccine in Mice Requires Both Antibodies and T Cells Cox, Freek Baart, Matthijs Huizingh, Jeroen Tolboom, Jeroen Dekking, Liesbeth Goudsmit, Jaap Saeland, Eirikur Radošević, Katarina PLoS One Research Article BACKGROUND: It remains important to develop the next generation of influenza vaccines that can provide protection against vaccine mismatched strains and to be prepared for potential pandemic outbreaks. To achieve this, the understanding of the immunological parameters that mediate such broad protection is crucial. METHOD: In the current study we assessed the contribution of humoral and cellular immune responses to heterosubtypic protection against H5N1 induced by a Matrix-M (MM) adjuvanted seasonal influenza vaccine by serum transfer and T-cell depletion studies. RESULTS: We demonstrate that the heterosubtypic protection against H5N1 induced by MM adjuvanted vaccine is partially mediated by antibodies. The serum contained both H5N1 cross-reactive hemagglutinin (HA)- and neuraminidase (NA)-specific antibodies but with limited virus neutralizing and no hemagglutination inhibiting activity. The cross-reactive antibodies induced antibody-dependent cellular cytotoxicity (ADCC) in vitro, suggesting a role for the Fc part of the antibodies in protection against H5N1. Besides H5N1 specific antibody responses, cross-reactive HA- and NA-specific T-cell responses were induced by the adjuvanted vaccine. T-cell depletion experiments demonstrated that both CD4(+) and CD8(+) T cells contribute to protection. CONCLUSION: Our study demonstrates that cross-protection against H5N1 induced by MM adjuvanted seasonal virosomal influenza vaccine requires both the humoral and cellular arm of the immune system. Public Library of Science 2015-12-22 /pmc/articles/PMC4687931/ /pubmed/26696245 http://dx.doi.org/10.1371/journal.pone.0145243 Text en © 2015 Cox et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Cox, Freek Baart, Matthijs Huizingh, Jeroen Tolboom, Jeroen Dekking, Liesbeth Goudsmit, Jaap Saeland, Eirikur Radošević, Katarina Protection against H5N1 Influenza Virus Induced by Matrix-M Adjuvanted Seasonal Virosomal Vaccine in Mice Requires Both Antibodies and T Cells |
title | Protection against H5N1 Influenza Virus Induced by Matrix-M Adjuvanted Seasonal Virosomal Vaccine in Mice Requires Both Antibodies and T Cells |
title_full | Protection against H5N1 Influenza Virus Induced by Matrix-M Adjuvanted Seasonal Virosomal Vaccine in Mice Requires Both Antibodies and T Cells |
title_fullStr | Protection against H5N1 Influenza Virus Induced by Matrix-M Adjuvanted Seasonal Virosomal Vaccine in Mice Requires Both Antibodies and T Cells |
title_full_unstemmed | Protection against H5N1 Influenza Virus Induced by Matrix-M Adjuvanted Seasonal Virosomal Vaccine in Mice Requires Both Antibodies and T Cells |
title_short | Protection against H5N1 Influenza Virus Induced by Matrix-M Adjuvanted Seasonal Virosomal Vaccine in Mice Requires Both Antibodies and T Cells |
title_sort | protection against h5n1 influenza virus induced by matrix-m adjuvanted seasonal virosomal vaccine in mice requires both antibodies and t cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4687931/ https://www.ncbi.nlm.nih.gov/pubmed/26696245 http://dx.doi.org/10.1371/journal.pone.0145243 |
work_keys_str_mv | AT coxfreek protectionagainsth5n1influenzavirusinducedbymatrixmadjuvantedseasonalvirosomalvaccineinmicerequiresbothantibodiesandtcells AT baartmatthijs protectionagainsth5n1influenzavirusinducedbymatrixmadjuvantedseasonalvirosomalvaccineinmicerequiresbothantibodiesandtcells AT huizinghjeroen protectionagainsth5n1influenzavirusinducedbymatrixmadjuvantedseasonalvirosomalvaccineinmicerequiresbothantibodiesandtcells AT tolboomjeroen protectionagainsth5n1influenzavirusinducedbymatrixmadjuvantedseasonalvirosomalvaccineinmicerequiresbothantibodiesandtcells AT dekkingliesbeth protectionagainsth5n1influenzavirusinducedbymatrixmadjuvantedseasonalvirosomalvaccineinmicerequiresbothantibodiesandtcells AT goudsmitjaap protectionagainsth5n1influenzavirusinducedbymatrixmadjuvantedseasonalvirosomalvaccineinmicerequiresbothantibodiesandtcells AT saelandeirikur protectionagainsth5n1influenzavirusinducedbymatrixmadjuvantedseasonalvirosomalvaccineinmicerequiresbothantibodiesandtcells AT radosevickatarina protectionagainsth5n1influenzavirusinducedbymatrixmadjuvantedseasonalvirosomalvaccineinmicerequiresbothantibodiesandtcells |