Preparation of inocula for experimental infection of blood with Streptococcus pneumoniae

Experimental infections of either cells or animals require the preparation of good quality inocula. Unfortunately, the important pulmonary pathogen Streptococcus pneumoniae • Media culture buffering extends S. pneumoniae viability for 6 h. • Washing before freezing of single use aliquots minimizes m...

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Detalles Bibliográficos
Autores principales: Vivas-Alegre, Santiago, Fernández-Natal, Isabel, López-Fidalgo, Eduardo, Rivero-Lezcano, Octavio Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Immunology and Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4688400/
https://www.ncbi.nlm.nih.gov/pubmed/26844211
http://dx.doi.org/10.1016/j.mex.2015.11.003
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author Vivas-Alegre, Santiago
Fernández-Natal, Isabel
López-Fidalgo, Eduardo
Rivero-Lezcano, Octavio Miguel
author_facet Vivas-Alegre, Santiago
Fernández-Natal, Isabel
López-Fidalgo, Eduardo
Rivero-Lezcano, Octavio Miguel
author_sort Vivas-Alegre, Santiago
collection PubMed
description Experimental infections of either cells or animals require the preparation of good quality inocula. Unfortunately, the important pulmonary pathogen Streptococcus pneumoniae • Media culture buffering extends S. pneumoniae viability for 6 h. • Washing before freezing of single use aliquots minimizes manipulation after thawing. • Experimental infection with the frozen inocula has shown that plasma has higher bactericidal activity than blood.
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spelling pubmed-46884002016-02-03 Preparation of inocula for experimental infection of blood with Streptococcus pneumoniae Vivas-Alegre, Santiago Fernández-Natal, Isabel López-Fidalgo, Eduardo Rivero-Lezcano, Octavio Miguel MethodsX Immunology and Microbiology Experimental infections of either cells or animals require the preparation of good quality inocula. Unfortunately, the important pulmonary pathogen Streptococcus pneumoniae • Media culture buffering extends S. pneumoniae viability for 6 h. • Washing before freezing of single use aliquots minimizes manipulation after thawing. • Experimental infection with the frozen inocula has shown that plasma has higher bactericidal activity than blood. Elsevier 2015-11-21 /pmc/articles/PMC4688400/ /pubmed/26844211 http://dx.doi.org/10.1016/j.mex.2015.11.003 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Immunology and Microbiology
Vivas-Alegre, Santiago
Fernández-Natal, Isabel
López-Fidalgo, Eduardo
Rivero-Lezcano, Octavio Miguel
Preparation of inocula for experimental infection of blood with Streptococcus pneumoniae
title Preparation of inocula for experimental infection of blood with Streptococcus pneumoniae
title_full Preparation of inocula for experimental infection of blood with Streptococcus pneumoniae
title_fullStr Preparation of inocula for experimental infection of blood with Streptococcus pneumoniae
title_full_unstemmed Preparation of inocula for experimental infection of blood with Streptococcus pneumoniae
title_short Preparation of inocula for experimental infection of blood with Streptococcus pneumoniae
title_sort preparation of inocula for experimental infection of blood with streptococcus pneumoniae
topic Immunology and Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4688400/
https://www.ncbi.nlm.nih.gov/pubmed/26844211
http://dx.doi.org/10.1016/j.mex.2015.11.003
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