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Modeling Kidney Disease with iPS Cells

Induced pluripotent stem cells (iPSCs) are somatic cells that have been transcriptionally reprogrammed to an embryonic stem cell (ESC)-like state. iPSCs are a renewable source of diverse somatic cell types and tissues matching the original patient, including nephron-like kidney organoids. iPSCs have...

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Detalles Bibliográficos
Autor principal: Freedman, Benjamin S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689367/
https://www.ncbi.nlm.nih.gov/pubmed/26740740
http://dx.doi.org/10.4137/BMI.S20054
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author Freedman, Benjamin S.
author_facet Freedman, Benjamin S.
author_sort Freedman, Benjamin S.
collection PubMed
description Induced pluripotent stem cells (iPSCs) are somatic cells that have been transcriptionally reprogrammed to an embryonic stem cell (ESC)-like state. iPSCs are a renewable source of diverse somatic cell types and tissues matching the original patient, including nephron-like kidney organoids. iPSCs have been derived representing several kidney disorders, such as ADPKD, ARPKD, Alport syndrome, and lupus nephritis, with the goals of generating replacement tissue and ‘disease in a dish’ laboratory models. Cellular defects in iPSCs and derived kidney organoids provide functional, personalized biomarkers, which can be correlated with genetic and clinical information. In proof of principle, disease-specific phenotypes have been described in iPSCs and ESCs with mutations linked to polycystic kidney disease or focal segmental glomerulosclerosis. In addition, these cells can be used to model nephrotoxic chemical injury. Recent advances in directed differentiation and CRISPR genome editing enable more specific iPSC models and present new possibilities for diagnostics, disease modeling, therapeutic screens, and tissue regeneration using human cells. This review outlines growth opportunities and design strategies for this rapidly expanding and evolving field.
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spelling pubmed-46893672016-01-06 Modeling Kidney Disease with iPS Cells Freedman, Benjamin S. Biomark Insights Review Induced pluripotent stem cells (iPSCs) are somatic cells that have been transcriptionally reprogrammed to an embryonic stem cell (ESC)-like state. iPSCs are a renewable source of diverse somatic cell types and tissues matching the original patient, including nephron-like kidney organoids. iPSCs have been derived representing several kidney disorders, such as ADPKD, ARPKD, Alport syndrome, and lupus nephritis, with the goals of generating replacement tissue and ‘disease in a dish’ laboratory models. Cellular defects in iPSCs and derived kidney organoids provide functional, personalized biomarkers, which can be correlated with genetic and clinical information. In proof of principle, disease-specific phenotypes have been described in iPSCs and ESCs with mutations linked to polycystic kidney disease or focal segmental glomerulosclerosis. In addition, these cells can be used to model nephrotoxic chemical injury. Recent advances in directed differentiation and CRISPR genome editing enable more specific iPSC models and present new possibilities for diagnostics, disease modeling, therapeutic screens, and tissue regeneration using human cells. This review outlines growth opportunities and design strategies for this rapidly expanding and evolving field. Libertas Academica 2015-12-22 /pmc/articles/PMC4689367/ /pubmed/26740740 http://dx.doi.org/10.4137/BMI.S20054 Text en © 2015 the author(s), publisher and licensee Libertas Academica Ltd. This is an open-access article distributed under the terms of the Creative Commons CC-BY-NC 3.0 License.
spellingShingle Review
Freedman, Benjamin S.
Modeling Kidney Disease with iPS Cells
title Modeling Kidney Disease with iPS Cells
title_full Modeling Kidney Disease with iPS Cells
title_fullStr Modeling Kidney Disease with iPS Cells
title_full_unstemmed Modeling Kidney Disease with iPS Cells
title_short Modeling Kidney Disease with iPS Cells
title_sort modeling kidney disease with ips cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689367/
https://www.ncbi.nlm.nih.gov/pubmed/26740740
http://dx.doi.org/10.4137/BMI.S20054
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