Cargando…
Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species
Termites are eusocial insects related to cockroaches that feed on lignocellulose. These insects are key species in ecosystems since they recycle a large amount of nutrients but also are pests, exerting major economic impacts. Knowledge on the molecular pathways underlying reproduction, caste differe...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689415/ https://www.ncbi.nlm.nih.gov/pubmed/26698123 http://dx.doi.org/10.1371/journal.pone.0145596 |
_version_ | 1782406842583875584 |
---|---|
author | Dedeine, Franck Weinert, Lucy A. Bigot, Diane Josse, Thibaut Ballenghien, Marion Cahais, Vincent Galtier, Nicolas Gayral, Philippe |
author_facet | Dedeine, Franck Weinert, Lucy A. Bigot, Diane Josse, Thibaut Ballenghien, Marion Cahais, Vincent Galtier, Nicolas Gayral, Philippe |
author_sort | Dedeine, Franck |
collection | PubMed |
description | Termites are eusocial insects related to cockroaches that feed on lignocellulose. These insects are key species in ecosystems since they recycle a large amount of nutrients but also are pests, exerting major economic impacts. Knowledge on the molecular pathways underlying reproduction, caste differentiation or lignocellulose digestion would largely benefit from additional transcriptomic data. This study focused on transcriptomes of secondary reproductive females (nymphoid neotenics). Thirteen transcriptomes were used: 10 of Reticulitermes flavipes and R. grassei sequenced from a previous study, and two transcriptomes of R. lucifugus sequenced for the present study. After transcriptome assembly and read mapping, we examined interspecific variations of genes expressed by termites or gut microorganisms. A total of 18,323 orthologous gene clusters were detected. Functional annotation and taxonomic assignment were performed on a total of 41,287 predicted contigs in the three termite species. Between the termite species studied, functional categories of genes were comparable. Gene ontology (GO) terms analysis allowed the discovery of 9 cellulases and a total of 79 contigs potentially involved in 11 enzymatic activities used in wood metabolism. Altogether, results of this study illustrate the strong potential for the use of comparative interspecific transcriptomes, representing a complete resource for future studies including differentially expressed genes between castes or SNP analysis for population genetics. |
format | Online Article Text |
id | pubmed-4689415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46894152015-12-31 Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species Dedeine, Franck Weinert, Lucy A. Bigot, Diane Josse, Thibaut Ballenghien, Marion Cahais, Vincent Galtier, Nicolas Gayral, Philippe PLoS One Research Article Termites are eusocial insects related to cockroaches that feed on lignocellulose. These insects are key species in ecosystems since they recycle a large amount of nutrients but also are pests, exerting major economic impacts. Knowledge on the molecular pathways underlying reproduction, caste differentiation or lignocellulose digestion would largely benefit from additional transcriptomic data. This study focused on transcriptomes of secondary reproductive females (nymphoid neotenics). Thirteen transcriptomes were used: 10 of Reticulitermes flavipes and R. grassei sequenced from a previous study, and two transcriptomes of R. lucifugus sequenced for the present study. After transcriptome assembly and read mapping, we examined interspecific variations of genes expressed by termites or gut microorganisms. A total of 18,323 orthologous gene clusters were detected. Functional annotation and taxonomic assignment were performed on a total of 41,287 predicted contigs in the three termite species. Between the termite species studied, functional categories of genes were comparable. Gene ontology (GO) terms analysis allowed the discovery of 9 cellulases and a total of 79 contigs potentially involved in 11 enzymatic activities used in wood metabolism. Altogether, results of this study illustrate the strong potential for the use of comparative interspecific transcriptomes, representing a complete resource for future studies including differentially expressed genes between castes or SNP analysis for population genetics. Public Library of Science 2015-12-23 /pmc/articles/PMC4689415/ /pubmed/26698123 http://dx.doi.org/10.1371/journal.pone.0145596 Text en © 2015 Dedeine et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Dedeine, Franck Weinert, Lucy A. Bigot, Diane Josse, Thibaut Ballenghien, Marion Cahais, Vincent Galtier, Nicolas Gayral, Philippe Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species |
title | Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species |
title_full | Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species |
title_fullStr | Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species |
title_full_unstemmed | Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species |
title_short | Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species |
title_sort | comparative analysis of transcriptomes from secondary reproductives of three reticulitermes termite species |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689415/ https://www.ncbi.nlm.nih.gov/pubmed/26698123 http://dx.doi.org/10.1371/journal.pone.0145596 |
work_keys_str_mv | AT dedeinefranck comparativeanalysisoftranscriptomesfromsecondaryreproductivesofthreereticulitermestermitespecies AT weinertlucya comparativeanalysisoftranscriptomesfromsecondaryreproductivesofthreereticulitermestermitespecies AT bigotdiane comparativeanalysisoftranscriptomesfromsecondaryreproductivesofthreereticulitermestermitespecies AT jossethibaut comparativeanalysisoftranscriptomesfromsecondaryreproductivesofthreereticulitermestermitespecies AT ballenghienmarion comparativeanalysisoftranscriptomesfromsecondaryreproductivesofthreereticulitermestermitespecies AT cahaisvincent comparativeanalysisoftranscriptomesfromsecondaryreproductivesofthreereticulitermestermitespecies AT galtiernicolas comparativeanalysisoftranscriptomesfromsecondaryreproductivesofthreereticulitermestermitespecies AT gayralphilippe comparativeanalysisoftranscriptomesfromsecondaryreproductivesofthreereticulitermestermitespecies |