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Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species

Termites are eusocial insects related to cockroaches that feed on lignocellulose. These insects are key species in ecosystems since they recycle a large amount of nutrients but also are pests, exerting major economic impacts. Knowledge on the molecular pathways underlying reproduction, caste differe...

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Autores principales: Dedeine, Franck, Weinert, Lucy A., Bigot, Diane, Josse, Thibaut, Ballenghien, Marion, Cahais, Vincent, Galtier, Nicolas, Gayral, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689415/
https://www.ncbi.nlm.nih.gov/pubmed/26698123
http://dx.doi.org/10.1371/journal.pone.0145596
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author Dedeine, Franck
Weinert, Lucy A.
Bigot, Diane
Josse, Thibaut
Ballenghien, Marion
Cahais, Vincent
Galtier, Nicolas
Gayral, Philippe
author_facet Dedeine, Franck
Weinert, Lucy A.
Bigot, Diane
Josse, Thibaut
Ballenghien, Marion
Cahais, Vincent
Galtier, Nicolas
Gayral, Philippe
author_sort Dedeine, Franck
collection PubMed
description Termites are eusocial insects related to cockroaches that feed on lignocellulose. These insects are key species in ecosystems since they recycle a large amount of nutrients but also are pests, exerting major economic impacts. Knowledge on the molecular pathways underlying reproduction, caste differentiation or lignocellulose digestion would largely benefit from additional transcriptomic data. This study focused on transcriptomes of secondary reproductive females (nymphoid neotenics). Thirteen transcriptomes were used: 10 of Reticulitermes flavipes and R. grassei sequenced from a previous study, and two transcriptomes of R. lucifugus sequenced for the present study. After transcriptome assembly and read mapping, we examined interspecific variations of genes expressed by termites or gut microorganisms. A total of 18,323 orthologous gene clusters were detected. Functional annotation and taxonomic assignment were performed on a total of 41,287 predicted contigs in the three termite species. Between the termite species studied, functional categories of genes were comparable. Gene ontology (GO) terms analysis allowed the discovery of 9 cellulases and a total of 79 contigs potentially involved in 11 enzymatic activities used in wood metabolism. Altogether, results of this study illustrate the strong potential for the use of comparative interspecific transcriptomes, representing a complete resource for future studies including differentially expressed genes between castes or SNP analysis for population genetics.
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spelling pubmed-46894152015-12-31 Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species Dedeine, Franck Weinert, Lucy A. Bigot, Diane Josse, Thibaut Ballenghien, Marion Cahais, Vincent Galtier, Nicolas Gayral, Philippe PLoS One Research Article Termites are eusocial insects related to cockroaches that feed on lignocellulose. These insects are key species in ecosystems since they recycle a large amount of nutrients but also are pests, exerting major economic impacts. Knowledge on the molecular pathways underlying reproduction, caste differentiation or lignocellulose digestion would largely benefit from additional transcriptomic data. This study focused on transcriptomes of secondary reproductive females (nymphoid neotenics). Thirteen transcriptomes were used: 10 of Reticulitermes flavipes and R. grassei sequenced from a previous study, and two transcriptomes of R. lucifugus sequenced for the present study. After transcriptome assembly and read mapping, we examined interspecific variations of genes expressed by termites or gut microorganisms. A total of 18,323 orthologous gene clusters were detected. Functional annotation and taxonomic assignment were performed on a total of 41,287 predicted contigs in the three termite species. Between the termite species studied, functional categories of genes were comparable. Gene ontology (GO) terms analysis allowed the discovery of 9 cellulases and a total of 79 contigs potentially involved in 11 enzymatic activities used in wood metabolism. Altogether, results of this study illustrate the strong potential for the use of comparative interspecific transcriptomes, representing a complete resource for future studies including differentially expressed genes between castes or SNP analysis for population genetics. Public Library of Science 2015-12-23 /pmc/articles/PMC4689415/ /pubmed/26698123 http://dx.doi.org/10.1371/journal.pone.0145596 Text en © 2015 Dedeine et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dedeine, Franck
Weinert, Lucy A.
Bigot, Diane
Josse, Thibaut
Ballenghien, Marion
Cahais, Vincent
Galtier, Nicolas
Gayral, Philippe
Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species
title Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species
title_full Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species
title_fullStr Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species
title_full_unstemmed Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species
title_short Comparative Analysis of Transcriptomes from Secondary Reproductives of Three Reticulitermes Termite Species
title_sort comparative analysis of transcriptomes from secondary reproductives of three reticulitermes termite species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689415/
https://www.ncbi.nlm.nih.gov/pubmed/26698123
http://dx.doi.org/10.1371/journal.pone.0145596
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