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High-speed centrifugation induces aggregation of extracellular vesicles
Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high-speed centrifugation is often used. We show here, using electron microsc...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Co-Action Publishing
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689953/ https://www.ncbi.nlm.nih.gov/pubmed/26700615 http://dx.doi.org/10.3402/jev.v4.29509 |
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author | Linares, Romain Tan, Sisareuth Gounou, Céline Arraud, Nicolas Brisson, Alain R. |
author_facet | Linares, Romain Tan, Sisareuth Gounou, Céline Arraud, Nicolas Brisson, Alain R. |
author_sort | Linares, Romain |
collection | PubMed |
description | Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high-speed centrifugation is often used. We show here, using electron microscopy, receptor-specific gold labelling and flow cytometry, that high-speed centrifugation induces the formation of EV aggregates composed of a mixture of EVs of various phenotypes and morphologies. The presence of aggregates made of EVs of different phenotypes may lead to erroneous interpretation concerning the existence of EVs harbouring surface antigens from different cell origins. |
format | Online Article Text |
id | pubmed-4689953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Co-Action Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-46899532016-01-06 High-speed centrifugation induces aggregation of extracellular vesicles Linares, Romain Tan, Sisareuth Gounou, Céline Arraud, Nicolas Brisson, Alain R. J Extracell Vesicles Short Communication Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high-speed centrifugation is often used. We show here, using electron microscopy, receptor-specific gold labelling and flow cytometry, that high-speed centrifugation induces the formation of EV aggregates composed of a mixture of EVs of various phenotypes and morphologies. The presence of aggregates made of EVs of different phenotypes may lead to erroneous interpretation concerning the existence of EVs harbouring surface antigens from different cell origins. Co-Action Publishing 2015-12-23 /pmc/articles/PMC4689953/ /pubmed/26700615 http://dx.doi.org/10.3402/jev.v4.29509 Text en © 2015 Romain Linares et al. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Linares, Romain Tan, Sisareuth Gounou, Céline Arraud, Nicolas Brisson, Alain R. High-speed centrifugation induces aggregation of extracellular vesicles |
title | High-speed centrifugation induces aggregation of extracellular vesicles |
title_full | High-speed centrifugation induces aggregation of extracellular vesicles |
title_fullStr | High-speed centrifugation induces aggregation of extracellular vesicles |
title_full_unstemmed | High-speed centrifugation induces aggregation of extracellular vesicles |
title_short | High-speed centrifugation induces aggregation of extracellular vesicles |
title_sort | high-speed centrifugation induces aggregation of extracellular vesicles |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4689953/ https://www.ncbi.nlm.nih.gov/pubmed/26700615 http://dx.doi.org/10.3402/jev.v4.29509 |
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