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Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation

In pigs, successful embryo implantation is an important guarantee for producing litter size, and early embryonic loss occurring on day 12–30 of gestation critically affects the potential litter size. The implantation process is regulated by the expression of numerous genes, so comprehensive analysis...

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Autores principales: Lin, Haichao, Wang, Huaizhong, Wang, Yanping, Liu, Chang, Wang, Cheng, Guo, Jianfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690044/
https://www.ncbi.nlm.nih.gov/pubmed/26703736
http://dx.doi.org/10.3390/genes6041330
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author Lin, Haichao
Wang, Huaizhong
Wang, Yanping
Liu, Chang
Wang, Cheng
Guo, Jianfeng
author_facet Lin, Haichao
Wang, Huaizhong
Wang, Yanping
Liu, Chang
Wang, Cheng
Guo, Jianfeng
author_sort Lin, Haichao
collection PubMed
description In pigs, successful embryo implantation is an important guarantee for producing litter size, and early embryonic loss occurring on day 12–30 of gestation critically affects the potential litter size. The implantation process is regulated by the expression of numerous genes, so comprehensive analysis of the endometrium is necessary. In this study, RNA sequencing (RNA-Seq) technology is used to analyze endometrial tissues during early pregnancy. We investigated the changes of gene expression between three stages (day 12, 18, and 25) by multiple comparisons. There were 1557, 8951, and 2345 differentially expressed genes (DEGs) revealed between the different periods of implantation. We selected several genes for validation by the use of quantitative real-time RT-PCR. Bioinformatic analysis of differentially expressed genes in the endometrium revealed a number of biological processes and pathways potentially involved in embryo implantation in the pig, most noticeably cell proliferation, regulation of immune response, interaction of cytokine-cytokine receptors, and cell adhesion. These results showed that specific gene expression patterns reflect the different functions of the endometrium in three stages (maternal recognition, conceptus attachment, and embryo implantation). This study identified comprehensive transcriptomic profile in the porcine endometrium and thus could be a foundation for targeted studies of genes and pathways potentially involved in abnormal endometrial receptivity and embryo loss in early pregnancy.
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spelling pubmed-46900442015-12-30 Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation Lin, Haichao Wang, Huaizhong Wang, Yanping Liu, Chang Wang, Cheng Guo, Jianfeng Genes (Basel) Article In pigs, successful embryo implantation is an important guarantee for producing litter size, and early embryonic loss occurring on day 12–30 of gestation critically affects the potential litter size. The implantation process is regulated by the expression of numerous genes, so comprehensive analysis of the endometrium is necessary. In this study, RNA sequencing (RNA-Seq) technology is used to analyze endometrial tissues during early pregnancy. We investigated the changes of gene expression between three stages (day 12, 18, and 25) by multiple comparisons. There were 1557, 8951, and 2345 differentially expressed genes (DEGs) revealed between the different periods of implantation. We selected several genes for validation by the use of quantitative real-time RT-PCR. Bioinformatic analysis of differentially expressed genes in the endometrium revealed a number of biological processes and pathways potentially involved in embryo implantation in the pig, most noticeably cell proliferation, regulation of immune response, interaction of cytokine-cytokine receptors, and cell adhesion. These results showed that specific gene expression patterns reflect the different functions of the endometrium in three stages (maternal recognition, conceptus attachment, and embryo implantation). This study identified comprehensive transcriptomic profile in the porcine endometrium and thus could be a foundation for targeted studies of genes and pathways potentially involved in abnormal endometrial receptivity and embryo loss in early pregnancy. MDPI 2015-12-21 /pmc/articles/PMC4690044/ /pubmed/26703736 http://dx.doi.org/10.3390/genes6041330 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Haichao
Wang, Huaizhong
Wang, Yanping
Liu, Chang
Wang, Cheng
Guo, Jianfeng
Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
title Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
title_full Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
title_fullStr Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
title_full_unstemmed Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
title_short Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation
title_sort transcriptomic analysis of the porcine endometrium during embryo implantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690044/
https://www.ncbi.nlm.nih.gov/pubmed/26703736
http://dx.doi.org/10.3390/genes6041330
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