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Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers
Alpha-lipoic acid (α-LA) was evaluated in this study for its molecular mechanisms against liver oxidative damage and inflammatory responses induced by aflatoxin B(1) (AFB(1)). Birds were randomly allocated into four groups with different diets for three weeks: a basal diet, a 300 mg/kg α-LA suppleme...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690129/ https://www.ncbi.nlm.nih.gov/pubmed/26694462 http://dx.doi.org/10.3390/toxins7124879 |
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author | Ma, Qiugang Li, Yan Fan, Yu Zhao, Lihong Wei, Hua Ji, Cheng Zhang, Jianyun |
author_facet | Ma, Qiugang Li, Yan Fan, Yu Zhao, Lihong Wei, Hua Ji, Cheng Zhang, Jianyun |
author_sort | Ma, Qiugang |
collection | PubMed |
description | Alpha-lipoic acid (α-LA) was evaluated in this study for its molecular mechanisms against liver oxidative damage and inflammatory responses induced by aflatoxin B(1) (AFB(1)). Birds were randomly allocated into four groups with different diets for three weeks: a basal diet, a 300 mg/kg α-LA supplementation in a basal diet, a diet containing 74 μg/kg AFB(1), and 300 mg/kg α-LA supplementation in a diet containing 74 μg/kg AFB(1). In the AFB(1) group, the expression of GSH-P(X) mRNA was down-regulated (p < 0.05), and the levels of lipid peroxide and nitric oxide were increased (p < 0.05) in the chicken livers compared to those of the control group. Additionally, the mRNA level of the pro-inflammatory factor interleukin-6 was up-regulated significantly (p < 0.05), the protein expressions of both the nuclear factor kappa B (NF-κB) p65 and the inducible nitric oxide synthase were enhanced significantly (p < 0.05) in the AFB(1) group. All of these negative effects were inhibited by α-LA. These results indicate that α-LA may be effective in preventing hepatic oxidative stress, down-regulating the expression of hepatic pro-inflammatory cytokines, as well as inhibiting NF-κB expression. |
format | Online Article Text |
id | pubmed-4690129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-46901292015-12-30 Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers Ma, Qiugang Li, Yan Fan, Yu Zhao, Lihong Wei, Hua Ji, Cheng Zhang, Jianyun Toxins (Basel) Article Alpha-lipoic acid (α-LA) was evaluated in this study for its molecular mechanisms against liver oxidative damage and inflammatory responses induced by aflatoxin B(1) (AFB(1)). Birds were randomly allocated into four groups with different diets for three weeks: a basal diet, a 300 mg/kg α-LA supplementation in a basal diet, a diet containing 74 μg/kg AFB(1), and 300 mg/kg α-LA supplementation in a diet containing 74 μg/kg AFB(1). In the AFB(1) group, the expression of GSH-P(X) mRNA was down-regulated (p < 0.05), and the levels of lipid peroxide and nitric oxide were increased (p < 0.05) in the chicken livers compared to those of the control group. Additionally, the mRNA level of the pro-inflammatory factor interleukin-6 was up-regulated significantly (p < 0.05), the protein expressions of both the nuclear factor kappa B (NF-κB) p65 and the inducible nitric oxide synthase were enhanced significantly (p < 0.05) in the AFB(1) group. All of these negative effects were inhibited by α-LA. These results indicate that α-LA may be effective in preventing hepatic oxidative stress, down-regulating the expression of hepatic pro-inflammatory cytokines, as well as inhibiting NF-κB expression. MDPI 2015-12-14 /pmc/articles/PMC4690129/ /pubmed/26694462 http://dx.doi.org/10.3390/toxins7124879 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ma, Qiugang Li, Yan Fan, Yu Zhao, Lihong Wei, Hua Ji, Cheng Zhang, Jianyun Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers |
title | Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers |
title_full | Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers |
title_fullStr | Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers |
title_full_unstemmed | Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers |
title_short | Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers |
title_sort | molecular mechanisms of lipoic acid protection against aflatoxin b(1)-induced liver oxidative damage and inflammatory responses in broilers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690129/ https://www.ncbi.nlm.nih.gov/pubmed/26694462 http://dx.doi.org/10.3390/toxins7124879 |
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