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Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers

Alpha-lipoic acid (α-LA) was evaluated in this study for its molecular mechanisms against liver oxidative damage and inflammatory responses induced by aflatoxin B(1) (AFB(1)). Birds were randomly allocated into four groups with different diets for three weeks: a basal diet, a 300 mg/kg α-LA suppleme...

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Autores principales: Ma, Qiugang, Li, Yan, Fan, Yu, Zhao, Lihong, Wei, Hua, Ji, Cheng, Zhang, Jianyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690129/
https://www.ncbi.nlm.nih.gov/pubmed/26694462
http://dx.doi.org/10.3390/toxins7124879
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author Ma, Qiugang
Li, Yan
Fan, Yu
Zhao, Lihong
Wei, Hua
Ji, Cheng
Zhang, Jianyun
author_facet Ma, Qiugang
Li, Yan
Fan, Yu
Zhao, Lihong
Wei, Hua
Ji, Cheng
Zhang, Jianyun
author_sort Ma, Qiugang
collection PubMed
description Alpha-lipoic acid (α-LA) was evaluated in this study for its molecular mechanisms against liver oxidative damage and inflammatory responses induced by aflatoxin B(1) (AFB(1)). Birds were randomly allocated into four groups with different diets for three weeks: a basal diet, a 300 mg/kg α-LA supplementation in a basal diet, a diet containing 74 μg/kg AFB(1), and 300 mg/kg α-LA supplementation in a diet containing 74 μg/kg AFB(1). In the AFB(1) group, the expression of GSH-P(X) mRNA was down-regulated (p < 0.05), and the levels of lipid peroxide and nitric oxide were increased (p < 0.05) in the chicken livers compared to those of the control group. Additionally, the mRNA level of the pro-inflammatory factor interleukin-6 was up-regulated significantly (p < 0.05), the protein expressions of both the nuclear factor kappa B (NF-κB) p65 and the inducible nitric oxide synthase were enhanced significantly (p < 0.05) in the AFB(1) group. All of these negative effects were inhibited by α-LA. These results indicate that α-LA may be effective in preventing hepatic oxidative stress, down-regulating the expression of hepatic pro-inflammatory cytokines, as well as inhibiting NF-κB expression.
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spelling pubmed-46901292015-12-30 Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers Ma, Qiugang Li, Yan Fan, Yu Zhao, Lihong Wei, Hua Ji, Cheng Zhang, Jianyun Toxins (Basel) Article Alpha-lipoic acid (α-LA) was evaluated in this study for its molecular mechanisms against liver oxidative damage and inflammatory responses induced by aflatoxin B(1) (AFB(1)). Birds were randomly allocated into four groups with different diets for three weeks: a basal diet, a 300 mg/kg α-LA supplementation in a basal diet, a diet containing 74 μg/kg AFB(1), and 300 mg/kg α-LA supplementation in a diet containing 74 μg/kg AFB(1). In the AFB(1) group, the expression of GSH-P(X) mRNA was down-regulated (p < 0.05), and the levels of lipid peroxide and nitric oxide were increased (p < 0.05) in the chicken livers compared to those of the control group. Additionally, the mRNA level of the pro-inflammatory factor interleukin-6 was up-regulated significantly (p < 0.05), the protein expressions of both the nuclear factor kappa B (NF-κB) p65 and the inducible nitric oxide synthase were enhanced significantly (p < 0.05) in the AFB(1) group. All of these negative effects were inhibited by α-LA. These results indicate that α-LA may be effective in preventing hepatic oxidative stress, down-regulating the expression of hepatic pro-inflammatory cytokines, as well as inhibiting NF-κB expression. MDPI 2015-12-14 /pmc/articles/PMC4690129/ /pubmed/26694462 http://dx.doi.org/10.3390/toxins7124879 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Qiugang
Li, Yan
Fan, Yu
Zhao, Lihong
Wei, Hua
Ji, Cheng
Zhang, Jianyun
Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers
title Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers
title_full Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers
title_fullStr Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers
title_full_unstemmed Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers
title_short Molecular Mechanisms of Lipoic Acid Protection against Aflatoxin B(1)-Induced Liver Oxidative Damage and Inflammatory Responses in Broilers
title_sort molecular mechanisms of lipoic acid protection against aflatoxin b(1)-induced liver oxidative damage and inflammatory responses in broilers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690129/
https://www.ncbi.nlm.nih.gov/pubmed/26694462
http://dx.doi.org/10.3390/toxins7124879
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