Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles

A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by t...

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Detalles Bibliográficos
Autores principales: Luan, Yunxia, Chen, Jiayi, Li, Cheng, Xie, Gang, Fu, Hailong, Ma, Zhihong, Lu, Anxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690133/
https://www.ncbi.nlm.nih.gov/pubmed/26690477
http://dx.doi.org/10.3390/toxins7124883
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author Luan, Yunxia
Chen, Jiayi
Li, Cheng
Xie, Gang
Fu, Hailong
Ma, Zhihong
Lu, Anxiang
author_facet Luan, Yunxia
Chen, Jiayi
Li, Cheng
Xie, Gang
Fu, Hailong
Ma, Zhihong
Lu, Anxiang
author_sort Luan, Yunxia
collection PubMed
description A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection.
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spelling pubmed-46901332015-12-30 Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles Luan, Yunxia Chen, Jiayi Li, Cheng Xie, Gang Fu, Hailong Ma, Zhihong Lu, Anxiang Toxins (Basel) Article A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection. MDPI 2015-12-10 /pmc/articles/PMC4690133/ /pubmed/26690477 http://dx.doi.org/10.3390/toxins7124883 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luan, Yunxia
Chen, Jiayi
Li, Cheng
Xie, Gang
Fu, Hailong
Ma, Zhihong
Lu, Anxiang
Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles
title Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles
title_full Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles
title_fullStr Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles
title_full_unstemmed Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles
title_short Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles
title_sort highly sensitive colorimetric detection of ochratoxin a by a label-free aptamer and gold nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690133/
https://www.ncbi.nlm.nih.gov/pubmed/26690477
http://dx.doi.org/10.3390/toxins7124883
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