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Evaluation of Pulmonary Reperfusion Injury in Rats Undergoing Mesenteric Ischemia and Reperfusion and Protective Effect of Postconditioning on this Process

INTRODUCTION: Some publications have demonstrated the presence of lung reperfusion injury in mesenteric ischemia and reperfusion (I/R), but under to diverse methods. Postconditioning has been recognized as effective in preventing reperfusion injury in various organs and tissues. However, its effecti...

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Autores principales: dos Santos, Carlos Henrique Marques, Aydos, Ricardo Dutra, Nogueira Neto, Ed, Miiji, Luciana Nakao Odashiro, Cassino, Pedro Carvalho, Alves, Isadora Ishaq, Calheiros, Nádia Meneguesso, Garcia, Milena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Cirurgia Cardiovascular 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690657/
https://www.ncbi.nlm.nih.gov/pubmed/26735599
http://dx.doi.org/10.5935/1678-9741.20150067
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author dos Santos, Carlos Henrique Marques
Aydos, Ricardo Dutra
Nogueira Neto, Ed
Miiji, Luciana Nakao Odashiro
Cassino, Pedro Carvalho
Alves, Isadora Ishaq
Calheiros, Nádia Meneguesso
Garcia, Milena
author_facet dos Santos, Carlos Henrique Marques
Aydos, Ricardo Dutra
Nogueira Neto, Ed
Miiji, Luciana Nakao Odashiro
Cassino, Pedro Carvalho
Alves, Isadora Ishaq
Calheiros, Nádia Meneguesso
Garcia, Milena
author_sort dos Santos, Carlos Henrique Marques
collection PubMed
description INTRODUCTION: Some publications have demonstrated the presence of lung reperfusion injury in mesenteric ischemia and reperfusion (I/R), but under to diverse methods. Postconditioning has been recognized as effective in preventing reperfusion injury in various organs and tissues. However, its effectiveness has not been evaluated in the prevention of lung reperfusion injury after mesenteric ischemia and reperfusion. OBJECTIVE: To evaluate the presence of pulmonary reperfusion injury and the protective effect of ischemic postconditioning on lung parenchyma in rats submitted to mesenteric ischemia and reperfusion. METHODS: Thirty Wistar rats were distributed into three groups: group A (10 rats), which was held mesenteric ischemia (30 minutes) and reperfusion (60 minutes); group B (10 rats), ischemia and reperfusion, interspersed by postconditioning with two alternating cycles of reperfusion and reocclusion, for two minutes each; and group C (10 rats), ischemia and reperfusion interleaved by postconditioning with four alternating cycles of reperfusion and reocclusion of 30 seconds each. Finally, it was resected the upper lung lobe for histological analysis. RESULTS: There were mild lung lesions (grade 1) in all samples. There was no statistical difference between groups 1 and 2 (P>0.05). CONCLUSION: The mesenteric ischemia and reperfusion in rats for thirty and sixty minutes, respectively, caused mild reperfusion injury in lung. Postconditioning was not able to minimize the remote reperfusion injury and there was no difference comparing two cycles of two minutes with four cycles of 30 seconds.
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spelling pubmed-46906572015-12-28 Evaluation of Pulmonary Reperfusion Injury in Rats Undergoing Mesenteric Ischemia and Reperfusion and Protective Effect of Postconditioning on this Process dos Santos, Carlos Henrique Marques Aydos, Ricardo Dutra Nogueira Neto, Ed Miiji, Luciana Nakao Odashiro Cassino, Pedro Carvalho Alves, Isadora Ishaq Calheiros, Nádia Meneguesso Garcia, Milena Braz J Cardiovasc Surg Original Article INTRODUCTION: Some publications have demonstrated the presence of lung reperfusion injury in mesenteric ischemia and reperfusion (I/R), but under to diverse methods. Postconditioning has been recognized as effective in preventing reperfusion injury in various organs and tissues. However, its effectiveness has not been evaluated in the prevention of lung reperfusion injury after mesenteric ischemia and reperfusion. OBJECTIVE: To evaluate the presence of pulmonary reperfusion injury and the protective effect of ischemic postconditioning on lung parenchyma in rats submitted to mesenteric ischemia and reperfusion. METHODS: Thirty Wistar rats were distributed into three groups: group A (10 rats), which was held mesenteric ischemia (30 minutes) and reperfusion (60 minutes); group B (10 rats), ischemia and reperfusion, interspersed by postconditioning with two alternating cycles of reperfusion and reocclusion, for two minutes each; and group C (10 rats), ischemia and reperfusion interleaved by postconditioning with four alternating cycles of reperfusion and reocclusion of 30 seconds each. Finally, it was resected the upper lung lobe for histological analysis. RESULTS: There were mild lung lesions (grade 1) in all samples. There was no statistical difference between groups 1 and 2 (P>0.05). CONCLUSION: The mesenteric ischemia and reperfusion in rats for thirty and sixty minutes, respectively, caused mild reperfusion injury in lung. Postconditioning was not able to minimize the remote reperfusion injury and there was no difference comparing two cycles of two minutes with four cycles of 30 seconds. Sociedade Brasileira de Cirurgia Cardiovascular 2015 /pmc/articles/PMC4690657/ /pubmed/26735599 http://dx.doi.org/10.5935/1678-9741.20150067 Text en http://creativecommons.org/licenses/by/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Original Article
dos Santos, Carlos Henrique Marques
Aydos, Ricardo Dutra
Nogueira Neto, Ed
Miiji, Luciana Nakao Odashiro
Cassino, Pedro Carvalho
Alves, Isadora Ishaq
Calheiros, Nádia Meneguesso
Garcia, Milena
Evaluation of Pulmonary Reperfusion Injury in Rats Undergoing Mesenteric Ischemia and Reperfusion and Protective Effect of Postconditioning on this Process
title Evaluation of Pulmonary Reperfusion Injury in Rats Undergoing Mesenteric Ischemia and Reperfusion and Protective Effect of Postconditioning on this Process
title_full Evaluation of Pulmonary Reperfusion Injury in Rats Undergoing Mesenteric Ischemia and Reperfusion and Protective Effect of Postconditioning on this Process
title_fullStr Evaluation of Pulmonary Reperfusion Injury in Rats Undergoing Mesenteric Ischemia and Reperfusion and Protective Effect of Postconditioning on this Process
title_full_unstemmed Evaluation of Pulmonary Reperfusion Injury in Rats Undergoing Mesenteric Ischemia and Reperfusion and Protective Effect of Postconditioning on this Process
title_short Evaluation of Pulmonary Reperfusion Injury in Rats Undergoing Mesenteric Ischemia and Reperfusion and Protective Effect of Postconditioning on this Process
title_sort evaluation of pulmonary reperfusion injury in rats undergoing mesenteric ischemia and reperfusion and protective effect of postconditioning on this process
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690657/
https://www.ncbi.nlm.nih.gov/pubmed/26735599
http://dx.doi.org/10.5935/1678-9741.20150067
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