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Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges

Bone tissue engineering may provide an alternative to autograft, however scaffold optimisation is required to maximize bone ingrowth. In designing scaffolds, pore architecture is important and there is evidence that cells prefer a degree of non-uniformity. The aim of this study was to compare scaffo...

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Autores principales: Clarke, S. A., Choi, S. Y., McKechnie, Melanie, Burke, G., Dunne, N., Walker, G., Cunningham, E., Buchanan, F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690835/
https://www.ncbi.nlm.nih.gov/pubmed/26704539
http://dx.doi.org/10.1007/s10856-015-5630-0
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author Clarke, S. A.
Choi, S. Y.
McKechnie, Melanie
Burke, G.
Dunne, N.
Walker, G.
Cunningham, E.
Buchanan, F.
author_facet Clarke, S. A.
Choi, S. Y.
McKechnie, Melanie
Burke, G.
Dunne, N.
Walker, G.
Cunningham, E.
Buchanan, F.
author_sort Clarke, S. A.
collection PubMed
description Bone tissue engineering may provide an alternative to autograft, however scaffold optimisation is required to maximize bone ingrowth. In designing scaffolds, pore architecture is important and there is evidence that cells prefer a degree of non-uniformity. The aim of this study was to compare scaffolds derived from a natural porous marine sponge (Spongia agaricina) with unique architecture to those derived from a synthetic polyurethane foam. Hydroxyapatite scaffolds of 1 cm(3) were prepared via ceramic infiltration of a marine sponge and a polyurethane (PU) foam. Human foetal osteoblasts (hFOB) were seeded at 1 × 10(5) cells/scaffold for up to 14 days. Cytotoxicity, cell number, morphology and differentiation were investigated. PU-derived scaffolds had 84–91 % porosity and 99.99 % pore interconnectivity. In comparison marine sponge-derived scaffolds had 56–61 % porosity and 99.9 % pore interconnectivity. hFOB studies showed that a greater number of cells were found on marine sponge-derived scaffolds at than on the PU scaffold but there was no significant difference in cell differentiation. X-ray diffraction and inductively coupled plasma mass spectrometry showed that Si ions were released from the marine-derived scaffold. In summary, three dimensional porous constructs have been manufactured that support cell attachment, proliferation and differentiation but significantly more cells were seen on marine-derived scaffolds. This could be due both to the chemistry and pore architecture of the scaffolds with an additional biological stimulus from presence of Si ions. Further in vivo tests in orthotopic models are required but this marine-derived scaffold shows promise for applications in bone tissue engineering.
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spelling pubmed-46908352015-12-31 Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges Clarke, S. A. Choi, S. Y. McKechnie, Melanie Burke, G. Dunne, N. Walker, G. Cunningham, E. Buchanan, F. J Mater Sci Mater Med Biomaterials Synthesis and Characterization Bone tissue engineering may provide an alternative to autograft, however scaffold optimisation is required to maximize bone ingrowth. In designing scaffolds, pore architecture is important and there is evidence that cells prefer a degree of non-uniformity. The aim of this study was to compare scaffolds derived from a natural porous marine sponge (Spongia agaricina) with unique architecture to those derived from a synthetic polyurethane foam. Hydroxyapatite scaffolds of 1 cm(3) were prepared via ceramic infiltration of a marine sponge and a polyurethane (PU) foam. Human foetal osteoblasts (hFOB) were seeded at 1 × 10(5) cells/scaffold for up to 14 days. Cytotoxicity, cell number, morphology and differentiation were investigated. PU-derived scaffolds had 84–91 % porosity and 99.99 % pore interconnectivity. In comparison marine sponge-derived scaffolds had 56–61 % porosity and 99.9 % pore interconnectivity. hFOB studies showed that a greater number of cells were found on marine sponge-derived scaffolds at than on the PU scaffold but there was no significant difference in cell differentiation. X-ray diffraction and inductively coupled plasma mass spectrometry showed that Si ions were released from the marine-derived scaffold. In summary, three dimensional porous constructs have been manufactured that support cell attachment, proliferation and differentiation but significantly more cells were seen on marine-derived scaffolds. This could be due both to the chemistry and pore architecture of the scaffolds with an additional biological stimulus from presence of Si ions. Further in vivo tests in orthotopic models are required but this marine-derived scaffold shows promise for applications in bone tissue engineering. Springer US 2015-12-24 2016 /pmc/articles/PMC4690835/ /pubmed/26704539 http://dx.doi.org/10.1007/s10856-015-5630-0 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Biomaterials Synthesis and Characterization
Clarke, S. A.
Choi, S. Y.
McKechnie, Melanie
Burke, G.
Dunne, N.
Walker, G.
Cunningham, E.
Buchanan, F.
Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges
title Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges
title_full Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges
title_fullStr Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges
title_full_unstemmed Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges
title_short Osteogenic cell response to 3-D hydroxyapatite scaffolds developed via replication of natural marine sponges
title_sort osteogenic cell response to 3-d hydroxyapatite scaffolds developed via replication of natural marine sponges
topic Biomaterials Synthesis and Characterization
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4690835/
https://www.ncbi.nlm.nih.gov/pubmed/26704539
http://dx.doi.org/10.1007/s10856-015-5630-0
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