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Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis

Cnidarians, the extant sister group to bilateria, are well known for their impressive regenerative capacity. The sea anemone Nematostella vectensis is a well-established system for the study of development and evolution that is receiving increased attention for its regenerative capacity. Nematostell...

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Autores principales: Amiel, Aldine R., Johnston, Hereroa T., Nedoncelle, Karine, Warner, Jacob F., Ferreira, Solène, Röttinger, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691047/
https://www.ncbi.nlm.nih.gov/pubmed/26633371
http://dx.doi.org/10.3390/ijms161226100
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author Amiel, Aldine R.
Johnston, Hereroa T.
Nedoncelle, Karine
Warner, Jacob F.
Ferreira, Solène
Röttinger, Eric
author_facet Amiel, Aldine R.
Johnston, Hereroa T.
Nedoncelle, Karine
Warner, Jacob F.
Ferreira, Solène
Röttinger, Eric
author_sort Amiel, Aldine R.
collection PubMed
description Cnidarians, the extant sister group to bilateria, are well known for their impressive regenerative capacity. The sea anemone Nematostella vectensis is a well-established system for the study of development and evolution that is receiving increased attention for its regenerative capacity. Nematostella is able to regrow missing body parts within five to six days after its bisection, yet studies describing the morphological, cellular, and molecular events underlying this process are sparse and very heterogeneous in their experimental approaches. In this study, we lay down the basic framework to study oral regeneration in Nematostella vectensis. Using various imaging and staining techniques we characterize in detail the morphological, cellular, and global molecular events that define specific landmarks of this process. Furthermore, we describe in vivo assays to evaluate wound healing success and the initiation of pharynx reformation. Using our described landmarks for regeneration and in vivo assays, we analyze the effects of perturbing either transcription or cellular proliferation on the regenerative process. Interestingly, neither one of these experimental perturbations has major effects on wound closure, although they slightly delay or partially block it. We further show that while the inhibition of transcription blocks regeneration in a very early step, inhibiting cellular proliferation only affects later events such as pharynx reformation and tentacle elongation.
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spelling pubmed-46910472016-01-06 Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis Amiel, Aldine R. Johnston, Hereroa T. Nedoncelle, Karine Warner, Jacob F. Ferreira, Solène Röttinger, Eric Int J Mol Sci Article Cnidarians, the extant sister group to bilateria, are well known for their impressive regenerative capacity. The sea anemone Nematostella vectensis is a well-established system for the study of development and evolution that is receiving increased attention for its regenerative capacity. Nematostella is able to regrow missing body parts within five to six days after its bisection, yet studies describing the morphological, cellular, and molecular events underlying this process are sparse and very heterogeneous in their experimental approaches. In this study, we lay down the basic framework to study oral regeneration in Nematostella vectensis. Using various imaging and staining techniques we characterize in detail the morphological, cellular, and global molecular events that define specific landmarks of this process. Furthermore, we describe in vivo assays to evaluate wound healing success and the initiation of pharynx reformation. Using our described landmarks for regeneration and in vivo assays, we analyze the effects of perturbing either transcription or cellular proliferation on the regenerative process. Interestingly, neither one of these experimental perturbations has major effects on wound closure, although they slightly delay or partially block it. We further show that while the inhibition of transcription blocks regeneration in a very early step, inhibiting cellular proliferation only affects later events such as pharynx reformation and tentacle elongation. MDPI 2015-12-01 /pmc/articles/PMC4691047/ /pubmed/26633371 http://dx.doi.org/10.3390/ijms161226100 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Amiel, Aldine R.
Johnston, Hereroa T.
Nedoncelle, Karine
Warner, Jacob F.
Ferreira, Solène
Röttinger, Eric
Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis
title Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis
title_full Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis
title_fullStr Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis
title_full_unstemmed Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis
title_short Characterization of Morphological and Cellular Events Underlying Oral Regeneration in the Sea Anemone, Nematostella vectensis
title_sort characterization of morphological and cellular events underlying oral regeneration in the sea anemone, nematostella vectensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691047/
https://www.ncbi.nlm.nih.gov/pubmed/26633371
http://dx.doi.org/10.3390/ijms161226100
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