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RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator

The alternative sigma factor RpoN regulates many cell functions, such as motility, quorum sensing, and virulence in the opportunistic pathogen Pseudomonas aeruginosa (P. aeruginosa). P. aeruginosa often evolves rpoN-negative variants during the chronic infection in cystic fibrosis patients. It is un...

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Autores principales: Cai, Zhao, Liu, Yang, Chen, Yicai, Yam, Joey Kuok Hoong, Chew, Su Chuen, Chua, Song Lin, Wang, Ke, Givskov, Michael, Yang, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691050/
https://www.ncbi.nlm.nih.gov/pubmed/26633362
http://dx.doi.org/10.3390/ijms161226103
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author Cai, Zhao
Liu, Yang
Chen, Yicai
Yam, Joey Kuok Hoong
Chew, Su Chuen
Chua, Song Lin
Wang, Ke
Givskov, Michael
Yang, Liang
author_facet Cai, Zhao
Liu, Yang
Chen, Yicai
Yam, Joey Kuok Hoong
Chew, Su Chuen
Chua, Song Lin
Wang, Ke
Givskov, Michael
Yang, Liang
author_sort Cai, Zhao
collection PubMed
description The alternative sigma factor RpoN regulates many cell functions, such as motility, quorum sensing, and virulence in the opportunistic pathogen Pseudomonas aeruginosa (P. aeruginosa). P. aeruginosa often evolves rpoN-negative variants during the chronic infection in cystic fibrosis patients. It is unclear how RpoN interacts with other regulatory mechanisms to control virulence of P. aeruginosa. In this study, we show that RpoN modulates the function of PqsR, a quorum sensing receptor regulating production of virulence factors including the phenazine pyocyanin. The ∆rpoN mutant is able to synthesize 4-quinolone signal molecule HHQ but unable to activate PqsR and Pseudomonas quinolone signal (pqs) quorum sensing. The ∆rpoN mutant produces minimal level of pyocyanin and is unable to produce the anti-staphylococcal agents. Providing pqsR in trans in the ∆rpoN mutant restores its pqs quorum sensing and virulence factor production to the wild-type level. Our study provides evidence that RpoN has a regulatory effect on P. aeruginosa virulence through modulating the function of the PqsR quorum sensing regulator.
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spelling pubmed-46910502016-01-06 RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator Cai, Zhao Liu, Yang Chen, Yicai Yam, Joey Kuok Hoong Chew, Su Chuen Chua, Song Lin Wang, Ke Givskov, Michael Yang, Liang Int J Mol Sci Article The alternative sigma factor RpoN regulates many cell functions, such as motility, quorum sensing, and virulence in the opportunistic pathogen Pseudomonas aeruginosa (P. aeruginosa). P. aeruginosa often evolves rpoN-negative variants during the chronic infection in cystic fibrosis patients. It is unclear how RpoN interacts with other regulatory mechanisms to control virulence of P. aeruginosa. In this study, we show that RpoN modulates the function of PqsR, a quorum sensing receptor regulating production of virulence factors including the phenazine pyocyanin. The ∆rpoN mutant is able to synthesize 4-quinolone signal molecule HHQ but unable to activate PqsR and Pseudomonas quinolone signal (pqs) quorum sensing. The ∆rpoN mutant produces minimal level of pyocyanin and is unable to produce the anti-staphylococcal agents. Providing pqsR in trans in the ∆rpoN mutant restores its pqs quorum sensing and virulence factor production to the wild-type level. Our study provides evidence that RpoN has a regulatory effect on P. aeruginosa virulence through modulating the function of the PqsR quorum sensing regulator. MDPI 2015-11-30 /pmc/articles/PMC4691050/ /pubmed/26633362 http://dx.doi.org/10.3390/ijms161226103 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cai, Zhao
Liu, Yang
Chen, Yicai
Yam, Joey Kuok Hoong
Chew, Su Chuen
Chua, Song Lin
Wang, Ke
Givskov, Michael
Yang, Liang
RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator
title RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator
title_full RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator
title_fullStr RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator
title_full_unstemmed RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator
title_short RpoN Regulates Virulence Factors of Pseudomonas aeruginosa via Modulating the PqsR Quorum Sensing Regulator
title_sort rpon regulates virulence factors of pseudomonas aeruginosa via modulating the pqsr quorum sensing regulator
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691050/
https://www.ncbi.nlm.nih.gov/pubmed/26633362
http://dx.doi.org/10.3390/ijms161226103
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