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miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensii

miR-29a is a conserved miRNA that participates in bone formation and immune response in vertebrates. miR-29a of Pinctada martensii (Pm-miR-29a) was identified in the previous research though deep sequencing. In this report, the precise sequence of mature Pm-miR-29a was validated using miRNA rapid am...

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Autores principales: Tian, Rongrong, Zheng, Zhe, Huang, Ronglian, Jiao, Yu, Du, Xiaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691125/
https://www.ncbi.nlm.nih.gov/pubmed/26690410
http://dx.doi.org/10.3390/ijms161226182
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author Tian, Rongrong
Zheng, Zhe
Huang, Ronglian
Jiao, Yu
Du, Xiaodong
author_facet Tian, Rongrong
Zheng, Zhe
Huang, Ronglian
Jiao, Yu
Du, Xiaodong
author_sort Tian, Rongrong
collection PubMed
description miR-29a is a conserved miRNA that participates in bone formation and immune response in vertebrates. miR-29a of Pinctada martensii (Pm-miR-29a) was identified in the previous research though deep sequencing. In this report, the precise sequence of mature Pm-miR-29a was validated using miRNA rapid amplification of cDNA ends (miR-RACE) technology. The precursor sequence of Pm-miR-29a was predicted to have 87 bp. Stem loop qRT-PCR analysis showed that Pm-miR-29a was easily detected in all the tissues, although expressions in the mantle and gill were low. The microstructure showed the disrupted growth of the nacre after Pm-miR-29a over-expression, which was induced by mimic injection into P. martensii. Results of the target analysis indicated that neuropeptide Y receptor type 2 (Y2R) was the potential target of Pm-miR-29a. Meanwhile, Pm-miR-29a mimics could obviously inhibit the relative luciferase activity of the reporter containing 3′ UTR (Untranslated Regions) of the Y2R gene. Furthermore, the expression of Y2R was downregulated whereas expressions of interleukin 17 (IL-17) and nuclear factor κB (NF-κB) were upregulated after Pm-miR-29a over-expression in the mantle and gill, thereby suggesting that Pm-miR-29a could activate the immune response of the pearl oyster. Results showed that Pm-miR-29a was involved in nacre formation and immune response by regulating Y2R in pearl oyster P. martensii.
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spelling pubmed-46911252016-01-06 miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensii Tian, Rongrong Zheng, Zhe Huang, Ronglian Jiao, Yu Du, Xiaodong Int J Mol Sci Article miR-29a is a conserved miRNA that participates in bone formation and immune response in vertebrates. miR-29a of Pinctada martensii (Pm-miR-29a) was identified in the previous research though deep sequencing. In this report, the precise sequence of mature Pm-miR-29a was validated using miRNA rapid amplification of cDNA ends (miR-RACE) technology. The precursor sequence of Pm-miR-29a was predicted to have 87 bp. Stem loop qRT-PCR analysis showed that Pm-miR-29a was easily detected in all the tissues, although expressions in the mantle and gill were low. The microstructure showed the disrupted growth of the nacre after Pm-miR-29a over-expression, which was induced by mimic injection into P. martensii. Results of the target analysis indicated that neuropeptide Y receptor type 2 (Y2R) was the potential target of Pm-miR-29a. Meanwhile, Pm-miR-29a mimics could obviously inhibit the relative luciferase activity of the reporter containing 3′ UTR (Untranslated Regions) of the Y2R gene. Furthermore, the expression of Y2R was downregulated whereas expressions of interleukin 17 (IL-17) and nuclear factor κB (NF-κB) were upregulated after Pm-miR-29a over-expression in the mantle and gill, thereby suggesting that Pm-miR-29a could activate the immune response of the pearl oyster. Results showed that Pm-miR-29a was involved in nacre formation and immune response by regulating Y2R in pearl oyster P. martensii. MDPI 2015-12-10 /pmc/articles/PMC4691125/ /pubmed/26690410 http://dx.doi.org/10.3390/ijms161226182 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tian, Rongrong
Zheng, Zhe
Huang, Ronglian
Jiao, Yu
Du, Xiaodong
miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensii
title miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensii
title_full miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensii
title_fullStr miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensii
title_full_unstemmed miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensii
title_short miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensii
title_sort mir-29a participated in nacre formation and immune response by targeting y2r in pinctada martensii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691125/
https://www.ncbi.nlm.nih.gov/pubmed/26690410
http://dx.doi.org/10.3390/ijms161226182
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