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Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate

The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra...

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Autores principales: Chaudhary, Spandan, Chikara, Surendra K., Sharma, Mahesh C., Chaudhary, Abhinav, Alam Syed, Bakhtiyar, Chaudhary, Pooja S., Mehta, Aditya, Patel, Maulik, Ghosh, Arpita, Iriti, Marcello
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691151/
https://www.ncbi.nlm.nih.gov/pubmed/26694357
http://dx.doi.org/10.3390/ijms161226208
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author Chaudhary, Spandan
Chikara, Surendra K.
Sharma, Mahesh C.
Chaudhary, Abhinav
Alam Syed, Bakhtiyar
Chaudhary, Pooja S.
Mehta, Aditya
Patel, Maulik
Ghosh, Arpita
Iriti, Marcello
author_facet Chaudhary, Spandan
Chikara, Surendra K.
Sharma, Mahesh C.
Chaudhary, Abhinav
Alam Syed, Bakhtiyar
Chaudhary, Pooja S.
Mehta, Aditya
Patel, Maulik
Ghosh, Arpita
Iriti, Marcello
author_sort Chaudhary, Spandan
collection PubMed
description The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%–0.9% to 1.1%–1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants.
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spelling pubmed-46911512016-01-06 Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate Chaudhary, Spandan Chikara, Surendra K. Sharma, Mahesh C. Chaudhary, Abhinav Alam Syed, Bakhtiyar Chaudhary, Pooja S. Mehta, Aditya Patel, Maulik Ghosh, Arpita Iriti, Marcello Int J Mol Sci Article The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%–0.9% to 1.1%–1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants. MDPI 2015-12-15 /pmc/articles/PMC4691151/ /pubmed/26694357 http://dx.doi.org/10.3390/ijms161226208 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chaudhary, Spandan
Chikara, Surendra K.
Sharma, Mahesh C.
Chaudhary, Abhinav
Alam Syed, Bakhtiyar
Chaudhary, Pooja S.
Mehta, Aditya
Patel, Maulik
Ghosh, Arpita
Iriti, Marcello
Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate
title Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate
title_full Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate
title_fullStr Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate
title_full_unstemmed Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate
title_short Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate
title_sort elicitation of diosgenin production in trigonella foenum-graecum (fenugreek) seedlings by methyl jasmonate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691151/
https://www.ncbi.nlm.nih.gov/pubmed/26694357
http://dx.doi.org/10.3390/ijms161226208
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