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Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate
The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691151/ https://www.ncbi.nlm.nih.gov/pubmed/26694357 http://dx.doi.org/10.3390/ijms161226208 |
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author | Chaudhary, Spandan Chikara, Surendra K. Sharma, Mahesh C. Chaudhary, Abhinav Alam Syed, Bakhtiyar Chaudhary, Pooja S. Mehta, Aditya Patel, Maulik Ghosh, Arpita Iriti, Marcello |
author_facet | Chaudhary, Spandan Chikara, Surendra K. Sharma, Mahesh C. Chaudhary, Abhinav Alam Syed, Bakhtiyar Chaudhary, Pooja S. Mehta, Aditya Patel, Maulik Ghosh, Arpita Iriti, Marcello |
author_sort | Chaudhary, Spandan |
collection | PubMed |
description | The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%–0.9% to 1.1%–1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants. |
format | Online Article Text |
id | pubmed-4691151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-46911512016-01-06 Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate Chaudhary, Spandan Chikara, Surendra K. Sharma, Mahesh C. Chaudhary, Abhinav Alam Syed, Bakhtiyar Chaudhary, Pooja S. Mehta, Aditya Patel, Maulik Ghosh, Arpita Iriti, Marcello Int J Mol Sci Article The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%–0.9% to 1.1%–1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants. MDPI 2015-12-15 /pmc/articles/PMC4691151/ /pubmed/26694357 http://dx.doi.org/10.3390/ijms161226208 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chaudhary, Spandan Chikara, Surendra K. Sharma, Mahesh C. Chaudhary, Abhinav Alam Syed, Bakhtiyar Chaudhary, Pooja S. Mehta, Aditya Patel, Maulik Ghosh, Arpita Iriti, Marcello Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate |
title | Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate |
title_full | Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate |
title_fullStr | Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate |
title_full_unstemmed | Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate |
title_short | Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate |
title_sort | elicitation of diosgenin production in trigonella foenum-graecum (fenugreek) seedlings by methyl jasmonate |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691151/ https://www.ncbi.nlm.nih.gov/pubmed/26694357 http://dx.doi.org/10.3390/ijms161226208 |
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