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RNA structure replaces the need for U2AF2 in splicing
RNA secondary structure plays an integral role in catalytic, ribosomal, small nuclear, micro, and transfer RNAs. Discovering a prevalent role for secondary structure in pre-mRNAs has proven more elusive. By utilizing a variety of computational and biochemical approaches, we present evidence for a cl...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691745/ https://www.ncbi.nlm.nih.gov/pubmed/26566657 http://dx.doi.org/10.1101/gr.181008.114 |
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author | Lin, Chien-Ling Taggart, Allison J. Lim, Kian Huat Cygan, Kamil J. Ferraris, Luciana Creton, Robbert Huang, Yen-Tsung Fairbrother, William G. |
author_facet | Lin, Chien-Ling Taggart, Allison J. Lim, Kian Huat Cygan, Kamil J. Ferraris, Luciana Creton, Robbert Huang, Yen-Tsung Fairbrother, William G. |
author_sort | Lin, Chien-Ling |
collection | PubMed |
description | RNA secondary structure plays an integral role in catalytic, ribosomal, small nuclear, micro, and transfer RNAs. Discovering a prevalent role for secondary structure in pre-mRNAs has proven more elusive. By utilizing a variety of computational and biochemical approaches, we present evidence for a class of nuclear introns that relies upon secondary structure for correct splicing. These introns are defined by simple repeat expansions of complementary AC and GT dimers that co-occur at opposite boundaries of an intron to form a bridging structure that enforces correct splice site pairing. Remarkably, this class of introns does not require U2AF2, a core component of the spliceosome, for its processing. Phylogenetic analysis suggests that this mechanism was present in the ancestral vertebrate lineage prior to the divergence of tetrapods from teleosts. While largely lost from land dwelling vertebrates, this class of introns is found in 10% of all zebrafish genes. |
format | Online Article Text |
id | pubmed-4691745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46917452016-07-01 RNA structure replaces the need for U2AF2 in splicing Lin, Chien-Ling Taggart, Allison J. Lim, Kian Huat Cygan, Kamil J. Ferraris, Luciana Creton, Robbert Huang, Yen-Tsung Fairbrother, William G. Genome Res Research RNA secondary structure plays an integral role in catalytic, ribosomal, small nuclear, micro, and transfer RNAs. Discovering a prevalent role for secondary structure in pre-mRNAs has proven more elusive. By utilizing a variety of computational and biochemical approaches, we present evidence for a class of nuclear introns that relies upon secondary structure for correct splicing. These introns are defined by simple repeat expansions of complementary AC and GT dimers that co-occur at opposite boundaries of an intron to form a bridging structure that enforces correct splice site pairing. Remarkably, this class of introns does not require U2AF2, a core component of the spliceosome, for its processing. Phylogenetic analysis suggests that this mechanism was present in the ancestral vertebrate lineage prior to the divergence of tetrapods from teleosts. While largely lost from land dwelling vertebrates, this class of introns is found in 10% of all zebrafish genes. Cold Spring Harbor Laboratory Press 2016-01 /pmc/articles/PMC4691745/ /pubmed/26566657 http://dx.doi.org/10.1101/gr.181008.114 Text en © 2016 Lin et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Lin, Chien-Ling Taggart, Allison J. Lim, Kian Huat Cygan, Kamil J. Ferraris, Luciana Creton, Robbert Huang, Yen-Tsung Fairbrother, William G. RNA structure replaces the need for U2AF2 in splicing |
title | RNA structure replaces the need for U2AF2 in splicing |
title_full | RNA structure replaces the need for U2AF2 in splicing |
title_fullStr | RNA structure replaces the need for U2AF2 in splicing |
title_full_unstemmed | RNA structure replaces the need for U2AF2 in splicing |
title_short | RNA structure replaces the need for U2AF2 in splicing |
title_sort | rna structure replaces the need for u2af2 in splicing |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691745/ https://www.ncbi.nlm.nih.gov/pubmed/26566657 http://dx.doi.org/10.1101/gr.181008.114 |
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