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High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases

Next-generation RNA-sequencing (RNA-seq) has revolutionized transcriptome profiling, gene expression analysis, and RNA-based diagnostics. Here, we developed a new RNA-seq method that exploits thermostable group II intron reverse transcriptases (TGIRTs) and used it to profile human plasma RNAs. TGIRT...

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Detalles Bibliográficos
Autores principales: Qin, Yidan, Yao, Jun, Wu, Douglas C., Nottingham, Ryan M., Mohr, Sabine, Hunicke-Smith, Scott, Lambowitz, Alan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691826/
https://www.ncbi.nlm.nih.gov/pubmed/26554030
http://dx.doi.org/10.1261/rna.054809.115

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