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Assessing the performance of the Oxford Nanopore Technologies MinION

The Oxford Nanopore Technologies (ONT) MinION is a new sequencing technology that potentially offers read lengths of tens of kilobases (kb) limited only by the length of DNA molecules presented to it. The device has a low capital cost, is by far the most portable DNA sequencer available, and can pro...

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Detalles Bibliográficos
Autores principales: Laver, T., Harrison, J., O’Neill, P.A., Moore, K., Farbos, A., Paszkiewicz, K., Studholme, D.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691839/
https://www.ncbi.nlm.nih.gov/pubmed/26753127
http://dx.doi.org/10.1016/j.bdq.2015.02.001
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author Laver, T.
Harrison, J.
O’Neill, P.A.
Moore, K.
Farbos, A.
Paszkiewicz, K.
Studholme, D.J.
author_facet Laver, T.
Harrison, J.
O’Neill, P.A.
Moore, K.
Farbos, A.
Paszkiewicz, K.
Studholme, D.J.
author_sort Laver, T.
collection PubMed
description The Oxford Nanopore Technologies (ONT) MinION is a new sequencing technology that potentially offers read lengths of tens of kilobases (kb) limited only by the length of DNA molecules presented to it. The device has a low capital cost, is by far the most portable DNA sequencer available, and can produce data in real-time. It has numerous prospective applications including improving genome sequence assemblies and resolution of repeat-rich regions. Before such a technology is widely adopted, it is important to assess its performance and limitations in respect of throughput and accuracy. In this study we assessed the performance of the MinION by re-sequencing three bacterial genomes, with very different nucleotide compositions ranging from 28.6% to 70.7%; the high G + C strain was underrepresented in the sequencing reads. We estimate the error rate of the MinION (after base calling) to be 38.2%. Mean and median read lengths were 2 kb and 1 kb respectively, while the longest single read was 98 kb. The whole length of a 5 kb rRNA operon was covered by a single read. As the first nanopore-based single molecule sequencer available to researchers, the MinION is an exciting prospect; however, the current error rate limits its ability to compete with existing sequencing technologies, though we do show that MinION sequence reads can enhance contiguity of de novo assembly when used in conjunction with Illumina MiSeq data.
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spelling pubmed-46918392016-01-08 Assessing the performance of the Oxford Nanopore Technologies MinION Laver, T. Harrison, J. O’Neill, P.A. Moore, K. Farbos, A. Paszkiewicz, K. Studholme, D.J. Biomol Detect Quantif Original Article The Oxford Nanopore Technologies (ONT) MinION is a new sequencing technology that potentially offers read lengths of tens of kilobases (kb) limited only by the length of DNA molecules presented to it. The device has a low capital cost, is by far the most portable DNA sequencer available, and can produce data in real-time. It has numerous prospective applications including improving genome sequence assemblies and resolution of repeat-rich regions. Before such a technology is widely adopted, it is important to assess its performance and limitations in respect of throughput and accuracy. In this study we assessed the performance of the MinION by re-sequencing three bacterial genomes, with very different nucleotide compositions ranging from 28.6% to 70.7%; the high G + C strain was underrepresented in the sequencing reads. We estimate the error rate of the MinION (after base calling) to be 38.2%. Mean and median read lengths were 2 kb and 1 kb respectively, while the longest single read was 98 kb. The whole length of a 5 kb rRNA operon was covered by a single read. As the first nanopore-based single molecule sequencer available to researchers, the MinION is an exciting prospect; however, the current error rate limits its ability to compete with existing sequencing technologies, though we do show that MinION sequence reads can enhance contiguity of de novo assembly when used in conjunction with Illumina MiSeq data. Elsevier 2015-03-24 /pmc/articles/PMC4691839/ /pubmed/26753127 http://dx.doi.org/10.1016/j.bdq.2015.02.001 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Laver, T.
Harrison, J.
O’Neill, P.A.
Moore, K.
Farbos, A.
Paszkiewicz, K.
Studholme, D.J.
Assessing the performance of the Oxford Nanopore Technologies MinION
title Assessing the performance of the Oxford Nanopore Technologies MinION
title_full Assessing the performance of the Oxford Nanopore Technologies MinION
title_fullStr Assessing the performance of the Oxford Nanopore Technologies MinION
title_full_unstemmed Assessing the performance of the Oxford Nanopore Technologies MinION
title_short Assessing the performance of the Oxford Nanopore Technologies MinION
title_sort assessing the performance of the oxford nanopore technologies minion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691839/
https://www.ncbi.nlm.nih.gov/pubmed/26753127
http://dx.doi.org/10.1016/j.bdq.2015.02.001
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