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Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction
Epidemiological evidence indicates that assisted reproductive technologies (ART) may be associated with several epigenetic diseases such as Beckwith-Wiedemann syndrome (BWS) or Silver-Russell syndrome (SRS). Selection of sperm by density-gradients in ART has improved DNA integrity and sperm quality;...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692407/ https://www.ncbi.nlm.nih.gov/pubmed/26709917 http://dx.doi.org/10.1371/journal.pone.0145585 |
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author | Yu, Bolan Zhou, Hua Liu, Min Zheng, Ting Jiang, Lu Zhao, Mei Xu, Xiaoxie Huang, Zhaofeng |
author_facet | Yu, Bolan Zhou, Hua Liu, Min Zheng, Ting Jiang, Lu Zhao, Mei Xu, Xiaoxie Huang, Zhaofeng |
author_sort | Yu, Bolan |
collection | PubMed |
description | Epidemiological evidence indicates that assisted reproductive technologies (ART) may be associated with several epigenetic diseases such as Beckwith-Wiedemann syndrome (BWS) or Silver-Russell syndrome (SRS). Selection of sperm by density-gradients in ART has improved DNA integrity and sperm quality; however, epigenetic alterations associated with this approach are largely unknown. In the present study, we investigated DNA methylation and histone retention profiles in raw sperm and selected sperm derived from the same individual and separated by using density-gradients. Results from a study group consisting of 93 males demonstrated that both global DNA methylation and histone retention levels decreased in density selected sperm. Compared to unselected raw sperm, histone transition rates decreased by an average of 27.2% in selected sperm, and the global methylation rate was 3.8% in unselected sperm and 3.3% in the selected sperm. DNA methylation and histone retention location profiling analyses suggested that these alterations displayed specific location patterns in the human genome. Changes in the pattern of hypomethylation largely occurred in transcriptional factor gene families such as HOX, FOX, and GATA. Histone retention increased in 67 genes, whereas it was significantly clustered in neural development-related gene families, particularly the olfactory sensor gene family. Although a causative relationship could not be established, the results of the present study suggest the possibility that sperm with good density also possess unique epigenetic profiles, particularly for genes involved in neural and olfactory development. As increasing evidence demonstrates that epigenetics plays a key role in embryonic development and offspring growth characteristics, the specific epigenetic alterations we observed in selected sperm may influence the transcriptional process and neural development in embryos. |
format | Online Article Text |
id | pubmed-4692407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46924072016-01-12 Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction Yu, Bolan Zhou, Hua Liu, Min Zheng, Ting Jiang, Lu Zhao, Mei Xu, Xiaoxie Huang, Zhaofeng PLoS One Research Article Epidemiological evidence indicates that assisted reproductive technologies (ART) may be associated with several epigenetic diseases such as Beckwith-Wiedemann syndrome (BWS) or Silver-Russell syndrome (SRS). Selection of sperm by density-gradients in ART has improved DNA integrity and sperm quality; however, epigenetic alterations associated with this approach are largely unknown. In the present study, we investigated DNA methylation and histone retention profiles in raw sperm and selected sperm derived from the same individual and separated by using density-gradients. Results from a study group consisting of 93 males demonstrated that both global DNA methylation and histone retention levels decreased in density selected sperm. Compared to unselected raw sperm, histone transition rates decreased by an average of 27.2% in selected sperm, and the global methylation rate was 3.8% in unselected sperm and 3.3% in the selected sperm. DNA methylation and histone retention location profiling analyses suggested that these alterations displayed specific location patterns in the human genome. Changes in the pattern of hypomethylation largely occurred in transcriptional factor gene families such as HOX, FOX, and GATA. Histone retention increased in 67 genes, whereas it was significantly clustered in neural development-related gene families, particularly the olfactory sensor gene family. Although a causative relationship could not be established, the results of the present study suggest the possibility that sperm with good density also possess unique epigenetic profiles, particularly for genes involved in neural and olfactory development. As increasing evidence demonstrates that epigenetics plays a key role in embryonic development and offspring growth characteristics, the specific epigenetic alterations we observed in selected sperm may influence the transcriptional process and neural development in embryos. Public Library of Science 2015-12-28 /pmc/articles/PMC4692407/ /pubmed/26709917 http://dx.doi.org/10.1371/journal.pone.0145585 Text en © 2015 Yu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yu, Bolan Zhou, Hua Liu, Min Zheng, Ting Jiang, Lu Zhao, Mei Xu, Xiaoxie Huang, Zhaofeng Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction |
title | Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction |
title_full | Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction |
title_fullStr | Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction |
title_full_unstemmed | Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction |
title_short | Epigenetic Alterations in Density Selected Human Spermatozoa for Assisted Reproduction |
title_sort | epigenetic alterations in density selected human spermatozoa for assisted reproduction |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692407/ https://www.ncbi.nlm.nih.gov/pubmed/26709917 http://dx.doi.org/10.1371/journal.pone.0145585 |
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