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Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

Synaptic tagging and capture (STC) and cross-tagging are two important mechanisms at cellular level that explain how synapse-specificity and associativity is achieved in neurons within a specific time frame. These long-term plasticity-related processes are the leading candidate models to study the b...

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Autores principales: Shetty, Mahesh Shivarama, Sharma, Mahima, Hui, Neo Sin, Dasgupta, Ananya, Gopinadhan, Suma, Sajikumar, Sreedharan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692586/
https://www.ncbi.nlm.nih.gov/pubmed/26381286
http://dx.doi.org/10.3791/53008
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author Shetty, Mahesh Shivarama
Sharma, Mahima
Hui, Neo Sin
Dasgupta, Ananya
Gopinadhan, Suma
Sajikumar, Sreedharan
author_facet Shetty, Mahesh Shivarama
Sharma, Mahima
Hui, Neo Sin
Dasgupta, Ananya
Gopinadhan, Suma
Sajikumar, Sreedharan
author_sort Shetty, Mahesh Shivarama
collection PubMed
description Synaptic tagging and capture (STC) and cross-tagging are two important mechanisms at cellular level that explain how synapse-specificity and associativity is achieved in neurons within a specific time frame. These long-term plasticity-related processes are the leading candidate models to study the basis of memory formation and persistence at the cellular level. Both STC and cross-tagging involve two serial processes: (1) setting of the synaptic tag as triggered by a specific pattern of stimulation, and (2) synaptic capture, whereby the synaptic tag interacts with newly synthesized plasticity-related proteins (PRPs). Much of the understanding about the concepts of STC and cross-tagging arises from the studies done in CA1 region of the hippocampus and because of the technical complexity many of the laboratories are still unable to study these processes. Experimental conditions for the preparation of hippocampal slices and the recording of stable late-LTP/LTD are extremely important to study synaptic tagging/cross-tagging. This video article describes the experimental procedures to study long-term plasticity processes such as STC and cross-tagging in the CA1 pyramidal neurons using stable, long-term field-potential recordings from acute hippocampal slices of rats.
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spelling pubmed-46925862016-01-07 Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents Shetty, Mahesh Shivarama Sharma, Mahima Hui, Neo Sin Dasgupta, Ananya Gopinadhan, Suma Sajikumar, Sreedharan J Vis Exp Neuroscience Synaptic tagging and capture (STC) and cross-tagging are two important mechanisms at cellular level that explain how synapse-specificity and associativity is achieved in neurons within a specific time frame. These long-term plasticity-related processes are the leading candidate models to study the basis of memory formation and persistence at the cellular level. Both STC and cross-tagging involve two serial processes: (1) setting of the synaptic tag as triggered by a specific pattern of stimulation, and (2) synaptic capture, whereby the synaptic tag interacts with newly synthesized plasticity-related proteins (PRPs). Much of the understanding about the concepts of STC and cross-tagging arises from the studies done in CA1 region of the hippocampus and because of the technical complexity many of the laboratories are still unable to study these processes. Experimental conditions for the preparation of hippocampal slices and the recording of stable late-LTP/LTD are extremely important to study synaptic tagging/cross-tagging. This video article describes the experimental procedures to study long-term plasticity processes such as STC and cross-tagging in the CA1 pyramidal neurons using stable, long-term field-potential recordings from acute hippocampal slices of rats. MyJove Corporation 2015-09-04 /pmc/articles/PMC4692586/ /pubmed/26381286 http://dx.doi.org/10.3791/53008 Text en Copyright © 2015, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Neuroscience
Shetty, Mahesh Shivarama
Sharma, Mahima
Hui, Neo Sin
Dasgupta, Ananya
Gopinadhan, Suma
Sajikumar, Sreedharan
Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
title Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
title_full Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
title_fullStr Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
title_full_unstemmed Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
title_short Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
title_sort investigation of synaptic tagging/capture and cross-capture using acute hippocampal slices from rodents
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692586/
https://www.ncbi.nlm.nih.gov/pubmed/26381286
http://dx.doi.org/10.3791/53008
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