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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Measuring levels of mRNAs in the process of translation in individual cells provides information on the proteins involved in cellular functions at a given point in time. The protocol dubbed Translating Ribosome Affinity Purification (TRAP) is able to capture this mRNA translation process in a cell-t...

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Detalles Bibliográficos
Autores principales: Bertin, Benjamin, Renaud, Yoan, Aradhya, Rajaguru, Jagla, Krzysztof, Junion, Guillaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692598/
https://www.ncbi.nlm.nih.gov/pubmed/26381166
http://dx.doi.org/10.3791/52985