Cargando…

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

Measuring levels of mRNAs in the process of translation in individual cells provides information on the proteins involved in cellular functions at a given point in time. The protocol dubbed Translating Ribosome Affinity Purification (TRAP) is able to capture this mRNA translation process in a cell-t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Bertin, Benjamin, Renaud, Yoan, Aradhya, Rajaguru, Jagla, Krzysztof, Junion, Guillaume
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2015
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692598/ https://www.ncbi.nlm.nih.gov/pubmed/26381166 http://dx.doi.org/10.3791/52985

Ejemplares similares

Gelsolin and dCryAB act downstream of muscle identity genes and contribute to preventing muscle splitting and branching in Drosophila
por: Bertin, Benjamin, et al.
Publicado: (2021)

Dual-Color Live Imaging of Adult Muscle Stem Cells in the Embryonic Tissues of Drosophila melanogaster
por: Zmojdzian, Monika, et al.
Publicado: (2023)

Muscle niche-driven Insulin-Notch-Myc cascade reactivates dormant Adult Muscle Precursors in Drosophila
por: Aradhya, Rajaguru, et al.
Publicado: (2015)

The versatile interactome of chloroplast ribosomes revealed by affinity purification mass spectrometry
por: Westrich, Lisa Désirée, et al.
Publicado: (2020)

Quantification of mRNA ribosomal engagement in human neurons using parallel translating ribosome affinity purification (TRAP) and RNA sequencing
por: Rodrigues, Deivid Carvalho, et al.
Publicado: (2020)

Straightjacket/α2δ3 deregulation is associated with cardiac conduction defects in myotonic dystrophy type 1
por: Auxerre-Plantié, Emilie, et al.
Publicado: (2019)

Identification of Protein Interacting Partners Using Tandem Affinity Purification
por: Bailey, Dalan, et al.
Publicado: (2012)

A split green fluorescent protein system to enhance spatial and temporal sensitivity of translating ribosome affinity purification
por: Dinkeloo, Kasia, et al.
Publicado: (2022)

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
por: Nilvebrant, Johan, et al.
Publicado: (2012)

Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification
por: Han, Xingfa, et al.
Publicado: (2021)

Deregulations of miR‐1 and its target Multiplexin promote dilated cardiomyopathy associated with myotonic dystrophy type 1
por: Souidi, Anissa, et al.
Publicado: (2023)

Rapid identification of regulatory microRNAs by miTRAP (miRNA trapping by RNA in vitro affinity purification)
por: Braun, Juliane, et al.
Publicado: (2014)

Combining affinity purification and mass spectrometry to define the network of the nuclear proteins interacting with the N-terminal region of FMRP
por: Kieffer, Félicie, et al.
Publicado: (2022)

A protein expression system for tandem affinity purification in Xanthomonas citri subsp. citri
por: Dantas, Giordanni C., et al.
Publicado: (2016)

Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification
por: Imai, Hirotatsu, et al.
Publicado: (2023)

Commentary: History of the ribosome and the origin of translation
por: Caetano-Anollés, Derek, et al.
Publicado: (2017)

Identification of Drosophila centromere associated proteins by quantitative affinity purification-mass spectrometry
por: Barth, Teresa K., et al.
Publicado: (2015)

Optimization of Ribosome Footprinting Conditions for Ribo-Seq in Human and Drosophila melanogaster Tissue Culture Cells
por: Douka, Katerina, et al.
Publicado: (2022)

Transcriptomic and Genetic Analyses Identify the Krüppel-Like Factor Dar1 as a New Regulator of Tube-Shaped Long Tendon Development
por: Laurichesse, Quentin, et al.
Publicado: (2021)

Drosophila Heart as a Model for Cardiac Development and Diseases
por: Souidi, Anissa, et al.
Publicado: (2021)

The eukaryote-specific N-terminal extension of ribosomal protein S31 contributes to the assembly and function of 40S ribosomal subunits
por: Fernández-Pevida, Antonio, et al.
Publicado: (2016)

Monosomes buffer translational stress to allow for active ribosome elongation
por: Schieweck, Rico, et al.
Publicado: (2023)

Genetic Control of Muscle Diversification and Homeostasis: Insights from Drosophila
por: Poovathumkadavil, Preethi, et al.
Publicado: (2020)

Developmental Expression and Functions of the Small Heat Shock Proteins in Drosophila
por: Jagla, Teresa, et al.
Publicado: (2018)

Functional conservation between structurally diverse ribosomal proteins from Drosophila melanogaster and Saccharomyces cerevisiae: fly L23a can substitute for yeast L25 in ribosome assembly and function
por: Ross, Carrie L. N., et al.
Publicado: (2007)

Non-Affinity Purification of Antibodies
por: Arakawa, Tsutomu, et al.
Publicado: (2023)

Bruno-3 regulates sarcomere component expression and contributes to muscle phenotypes of myotonic dystrophy type 1
por: Picchio, Lucie, et al.
Publicado: (2018)

The extended loops of ribosomal proteins uL4 and uL22 of Escherichia coli contribute to ribosome assembly and protein translation
por: Lawrence, Marlon G., et al.
Publicado: (2016)

Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast
por: Rubio, Angela, et al.
Publicado: (2020)

Drosophila Hsp67Bc hot-spot variants alter muscle structure and function
por: Jabłońska, Jadwiga, et al.
Publicado: (2018)

Mutational analysis of the ribosome assembly GTPase RbgA provides insight into ribosome interaction and ribosome-stimulated GTPase activation
por: Gulati, Megha, et al.
Publicado: (2013)

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
por: Shirole, Nitin, et al.
Publicado: (2011)

A minimal bacterial RNase J-based degradosome is associated with translating ribosomes
por: Redko, Yulia, et al.
Publicado: (2013)

Distinct types of translation termination generate substrates for ribosome-associated quality control
por: Shcherbik, Natalia, et al.
Publicado: (2016)

miRNA suppression of a Notch repressor directs non-neuronal fate in Drosophila mechanosensory organs
por: Kavaler, Joshua, et al.
Publicado: (2018)

Structures of translationally inactive mammalian ribosomes
por: Brown, Alan, et al.
Publicado: (2018)

An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome
por: Florin, Tanja, et al.
Publicado: (2017)

Heparin affinity purification of extracellular vesicles
por: Balaj, Leonora, et al.
Publicado: (2015)

Affinity Membranes and Monoliths for Protein Purification
por: Lalli, Eleonora, et al.
Publicado: (2019)

Developmental origin of tendon diversity in Drosophila melanogaster
por: Moucaud, Blandine, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_ql3lrk09ptdtesklmulbugfru5