Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy

This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative fluorescence microscopy and environmental scanning electron microscopy (ESEM) of whole cells in hydrated state. Fluorescent quantum dots (QDs) were coupled to EGFR via...

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Detalles Bibliográficos
Autores principales: Peckys, Diana B., de Jonge, Niels
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692600/
https://www.ncbi.nlm.nih.gov/pubmed/26383083
http://dx.doi.org/10.3791/53186

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692600/
https://www.ncbi.nlm.nih.gov/pubmed/26383083
http://dx.doi.org/10.3791/53186

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