Cargando…

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Extracellular measurement of oxygen consumption and acid production is a simple and powerful way to monitor rates of respiration and glycolysis(1). Both mitochondrial (respiration) and non-mitochondrial (other redox) reactions consume oxygen, but these reactions can be easily distinguished by chemic...

Descripción completa

Detalles Bibliográficos
Autores principales:	Mookerjee, Shona A., Brand, Martin D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2015
Materias:	Biochemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4692795/ https://www.ncbi.nlm.nih.gov/pubmed/26709455 http://dx.doi.org/10.3791/53464

Ejemplares similares

Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements
por: Mookerjee, Shona A., et al.
Publicado: (2016)

Advances in the study of aerobic glycolytic effects in resistance to radiotherapy in malignant tumors
por: Liu, Yuyi, et al.
Publicado: (2023)

Altered expression profile of glycolytic enzymes during testicular ischemia reperfusion injury is associated with the p53/TIGAR pathway: effect of fructose 1,6-diphosphate
por: Al-Maghrebi, May, et al.
Publicado: (2016)

Measurement of average decoding rates of the 61 sense codons in vivo
por: Gardin, Justin, et al.
Publicado: (2014)

Ligand determinants of fatty acid activation of the pronociceptive ion channel TRPA1
por: Redmond, William John, et al.
Publicado: (2014)

High Resolution Measurement of the Glycolytic Rate
por: Bittner, Carla X., et al.
Publicado: (2010)

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
por: Naba, Alexandra, et al.
Publicado: (2015)

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
por: Barallobre-Barreiro, Javier, et al.
Publicado: (2017)

Measurement of the Absolute Magnitude and Time Courses of Mitochondrial Membrane Potential in Primary and Clonal Pancreatic Beta-Cells
por: Gerencser, Akos A., et al.
Publicado: (2016)

Butyrate and other short-chain fatty acids increase the rate of lipolysis in 3T3-L1 adipocytes
por: Rumberger, John M., et al.
Publicado: (2014)

Amino acids and acylcarnitine production by Chlorella vulgaris and Chlorella sorokiniana microalgae from wastewater culture
por: Ballesteros-Torres, Juan M., et al.
Publicado: (2019)

A secretory kinase complex regulates extracellular protein phosphorylation
por: Cui, Jixin, et al.
Publicado: (2015)

Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling
por: Nachtergaele, Sigrid, et al.
Publicado: (2013)

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
por: Caffrey, Martin, et al.
Publicado: (2010)

Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation
por: Plate, Lars, et al.
Publicado: (2016)

An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina
por: Visser, Jasper J, et al.
Publicado: (2015)

Recombinant expression in E. coli of human FGFR2 with its transmembrane and extracellular domains
por: Bajinting, Adam, et al.
Publicado: (2017)

The effect of cartilage decellularized extracellular matrix-chitosan compound on treating knee osteoarthritis in rats
por: Chen, Deng, et al.
Publicado: (2021)

Simple approach for ranking structure determining residues
por: Luna-Martínez, Oscar D., et al.
Publicado: (2016)

Data on the relation between renal biomarkers and measured glomerular filtration rate
por: Pottel, Hans, et al.
Publicado: (2017)

High fructose exposure modifies the amount of adipocyte-secreted microRNAs into extracellular vesicles in supernatants and plasma
por: Hernández-Díazcouder, Adrián, et al.
Publicado: (2021)

Structure of catalase determined by MicroED
por: Nannenga, Brent L, et al.
Publicado: (2014)

A general method for determining secondary active transporter substrate stoichiometry
por: Fitzgerald, Gabriel A, et al.
Publicado: (2017)

Biochemical parameters, dynamic tensiometry and circulating nucleic acids for cattle blood analysis: a review
por: Zaitsev, Sergei Yu., et al.
Publicado: (2020)

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)
por: Qi, Xiaoying, et al.
Publicado: (2018)

Structural determinants of nuclear export signal orientation in binding to exportin CRM1
por: Fung, Ho Yee Joyce, et al.
Publicado: (2015)

Hepatic bile acids and bile acid-related gene expression in pregnant and lactating rats
por: Zhu, Qiong N., et al.
Publicado: (2013)

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
por: Gunning, Yvonne, et al.
Publicado: (2016)

Determination of the affinity constants for phage display albumin-binding peptides
por: Shi, Yi-Feng
Publicado: (2023)

Influence of long term nitrogen limitation on lipid, protein and pigment production of Euglena gracilis in photoheterotrophic cultures
por: Tossavainen, Marika, et al.
Publicado: (2019)

LuxS-dependent AI-2 production is not involved in global regulation of natural product biosynthesis in Photorhabdus and Xenorhabdus
por: Heinrich, Antje K., et al.
Publicado: (2017)

Extracellular pH, osmolarity, temperature and humidity could discourage SARS-CoV-2 cell docking and propagation via intercellular signaling pathways
por: Cicconetti, Franco, et al.
Publicado: (2021)

A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences
por: Conlan, Jessica A., et al.
Publicado: (2017)

Measuring Protein Binding to F-actin by Co-sedimentation
por: Heier, Jonathon A., et al.
Publicado: (2017)

Quantitative determinants of aerobic glycolysis identify flux through the enzyme GAPDH as a limiting step
por: Shestov, Alexander A, et al.
Publicado: (2014)

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
por: Shoemaker, James D.
Publicado: (2010)

Luciferin production and luciferase transcription in the bioluminescent copepod Metridia lucens
por: Tessler, Michael, et al.
Publicado: (2018)

Proximate composition determination in goat cheese whey by near infrared spectroscopy (NIRS)
por: Galdino, Isadora Kaline Camelo Pires de Oliveira, et al.
Publicado: (2020)

Frequent, quantitative bone planar scintigraphy for determination of bone anabolism in growing mice
por: Zaloszyc, Ariane, et al.
Publicado: (2021)

Mitochondria and chloroplasts function in microalgae energy production
por: Gomez-Casati, Diego F., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_6bqmjhr4l2b844jq0v2b6cfmsc